...plasmids   Cytosine deaminase for RNA editing Feng Zhang’s lab previously developed a programmable adenosine-to-inosine...

...s41587-023-02050-w Yi, Z., Zhang, X., Wei, X., Li, J., Ren, J., Zhang, X., Zhang, Y., Tang, H., Chang, X....Hong, M., Luan, C., Gao, H., Ru, G., Guo, X., Zhang, D., Zhang, S., Li, C., Wu, J., Randolph, P. B., Sousa...., Qu, C., Zhu, Y., Guan, Y., Wang, L., Liu, M., Feng, B., Song, G., … Li, D. (2024). Adenine transversion...., Cui, S., Li, H., Li, G., Zhou, J., Yuan, Y., Zhang, H., Shi, L., Yao, X., & Yang, H. (2023). Programmable... H., Wei, Z., Liu, X., Li, Y., Li, S., Liu, Y., Zhang, X., & Bi, C. (2024). Glycosylase-based base editors...

...the MIT Sloan School of Management. Professor Feng Zhang’s original 2013 gene editing paper on CRISPR/Cas...In addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. ...

...Note: Cpf1 is also called Cas12a. In 2015, Feng Zhang’s lab characterized two Cpf1 nucleases, distant...regulatory elements. To solve this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) ...SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cpf1 Is a Single RNA-Guided Endonuclease of a...

...blast is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., ...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

...of our first blog post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung...potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018...

... Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About The Krüppel-like factors (KLFs) are a family...

...website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9...of a sunny day in Edinburgh.   References 1. Gao, Feng, et al. "DNA-guided genome editing using the Natronobacterium...

...players in the CRISPR field. Just a year after Feng Zhang’s lab identified Cas13a (C2c2) (Abudayyeh et al...end joining (NHEJ). Designing an RNA editor The Zhang lab envisioned a two-component RNA editor: a Cas13...Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA editing toolkit to allow C ...

...CRISPR-Cpf1 genome editing!  A recent paper from Feng Zhang's lab describes how to use Cpf1 for multiplex ...Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease...

...opens in a new window) . Zhang, J., Chen, B., Gan, C., Sun, H., Zhang, J., & Feng, L. (2023). A comprehensive...

... derived from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis...

...field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang’s group at the Broad Institute, will be available...available from Addgene before the end of the year. Dr. Zhang ‘s lab published a paper on TAL effectors in Nature...targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ,...

...) Cas13a targeting with a single crRNA The Feng Zhang lab used Cas13a from Leptotrichia shahii (LshCas13a...Ladha et al., 2020). Cas13a in mammalian cells The Zhang lab made the exciting discovery that some Cas13a...

...Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang...mutations in Bombyx mori and Drosophila melanogaster . Zhang Lab TALE Toolbox 49622 - 49647 24 plasmids Thoru...

...Addgene Zhang Lab CRISPR Resources Tips for and FAQs about the Zhang Lab CRISPRs Browse All Zhang Lab Plasmids... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang Lab CRISPR Plasmids...sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome Editing The Zhang Lab ...S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell . 2013 Sep 12;154(6):1380-9. doi: 10.1016...Langer 9, Anderson DG, Hacohen N, Regev A, Feng G, Sharp PA, Zhang F. Cell . 2014 Oct 9;159(2):440-55. doi...plasmids from the Zhang lab Genome...Plasmids Zhang Lab Website You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR...

...facilitate its packaging into virus-like particles. Feng Zhang’s lab adapted the native PEG10 RNA sequences to...

... window) A Google groups forum started by the Feng Zhang Lab . Protocols Lab(s) Description Plasmids in...pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF, 597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9...: Cas9 (pX330) or Cas9 D10A (pX335) PDF, 255 KB Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids:...psPAX2 ; positive control: CMV-EGFP PDF, 2.4 MB Zhang GeCKO pooled library amplification 1 vector system...

...SOD1) SOD1 hSyn1 ALS Feng Zhang 109322 pU6-crRNA(TBK1) TBK1 hSyn1 ALS Feng Zhang 109323 pU6-crRNA(TARDBP...FXN EF1a Friedreich ataxia Feng Zhang 142079 TFORF2441 FUS E1Fa ALS Feng Zhang 142463 TFORF0142 FXN E1Fa...E1Fa Friedreich ataxia Feng Zhang 142874 TFORF1856 NR4A2 E1Fa Parkinson's Feng Zhang 142875 TFORF1857 NR4A2...NR4A2 E1Fa Parkinson's Feng Zhang 142944 TFORF2440 FUS E1Fa ALS Feng Zhang 143027 TFORF0449 DNMT1 E1Fa ...sensory neuropathy type IE Feng Zhang 143542 TFORF1771 TBP E1Fa Parkinson's Feng Zhang 143543 TFORF1772 TBP ...Parkinson's Feng Zhang 143702 TFORF0450 DNMT1 E1Fa Hereditary sensory neuropathy type IE Feng Zhang 144906 ...Dong-Er Zhang 12421 pMCSF-R(mA)-luc CSF1R CSF1R Hereditary diffuse leukoencephalopathy Dong-Er Zhang 12422...

...played in these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden...