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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 103 results for: GRI;

Showing: 21 - 40 of 103 results

  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...However, two channels can be used to examine the integrity of the viral genome (Furuta-Hanawa et al., 2019...droplet digital PCR as a tool for titration and integrity evaluation of recombinant adeno-associated viral...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...over time, inactivating the protein binding it (Grimwade et al., 2018). Rather than serving as an energy...https://doi.org/10.1128/MMBR.62.2.434-464.1998 Grimwade, J. E., Rozgaja, T. A., Gupta, R., Dyson, K., ...

  4. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...full, compared to 50% of wild-type AAV2 stocks (Grimm et al., 1999). The percentage of genome-containing... 22428975. PubMed Central PMCID: PMC3640491. 2. Grimm, D., et al. "Titration of AAV-2 particles via a ...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection of an RNA...Nilaratanakul, Voraphoj, Debra A. Hauer, and Diane E. Griffin. "Development and characterization of sindbis virus...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...used these Opto-RhoGEFs to study cell junction integrity in the vascular barrier of the endothelium, but....org/10.7554/eLife.84364.  Meiring, J. C. M., Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...development of disease-resistant crops to increase agricultural yield (Wang et al., 2016; Wang et al., 2014)..., Gao C (2020) Applications of CRISPR–Cas in agriculture and plant biotechnology. Nat Rev Mol Cell Biol...

  8. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ...Niemi, Ph.D., is a postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin. ...

  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1 integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin...receptor2 Laminin receptor (LR)3 Putative integrin3 1Wu, Miller, et al., 20062Pillay et al.,...

  10. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under Creative Commons...https://doi.org/10.1128/JVI.76.22.11605-11611.2002 Griffin, J. M., Fackelmeier, B., Fong, D. M., Mouravlev...

  11. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... labeling tag, from Roger Tsien’s lab in 1998 (Griffin et al., 1998), this 15 amino acid peptide contains...https://doi.org/10.1016/j.chembiol.2008.01.007 Griffin, B. A., Adams, S. R., & Tsien, R. Y. (1998). Specific...

  12. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...cell surface and secreted proteins (glypicans, integrins, and more coming soon) as well as epitope tags...Antibodies 101: Producing Recombinant Antibodies An Integrin Antibody Toolkit from IPI Additional resources...

  14. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...heterodimerizing cryptochrome 2 (CRY2) and calcium and integrin-binding protein 1 (CIB1) proteins. The goal of...Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D (2018) Engineered...

  16. Rett Syndrome

    Type
    Collection
    ...Link opens in a new window) PMID: 35148843 Joost Gribnau Xist 2lox/2lox Conditional Xist, Lox sites flanking...conditional deletion of Xist Unpublished Joost Gribnau Human Cell Line Models Induced pluripotent stem...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids Oliver Griesbeck Calcium erGAP3 (GFP-Aequorin... Cell Biol. 2010 Feb 22;188(4):547-63. Sergio Grinstein pH Superfolder pHluorin pH sensor A superfolder...

  19. A Scientist's Guide to Ebola

    Type
    Blog Post
    ..., primarily in West African countries. Even that grim estimate might prove to be an underestimate. Given...
Showing: 21 - 40 of 103 results