We narrowed to 64 results for: Gateway
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TypeBlog Post...methods (restriction enzyme, ligation independent, Gateway, Gibson, etc). The cloning method is ultimately...
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Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...inactivates or completely removes (in the case of Gateway cloning) one cassette. Counterselection allows ... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...chemical stimulation was sucrose…..sounds like the gateway to chocolate and peanut butter to me. What’s on... -
Resolute Plasmid Collection
TypeCollection...RESOLUTE Plasmid Collection You may also like... Gateway Cloning Brain Initiative Collection NeuroMab Collection...codon-optimized ORF sequences for human SLCs in Gateway-compatible pENTR vectors (pDONR221). These vectors...and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post...repression), and Fok1-dCas9 (dimeric nuclease). Gateway assembly method Frew Lab Multiple Lentiviral Expression...should be compatible with type IIS enzyme BfuAI. Gateway cloning is then used to combine the multiple gRNAs... -
Lentivirus Plasmids
TypeCollection... plasmid. Cheng 17452 pLenti CMV Puro DEST 3rd Gateway destination plasmid with constitutive or inducible... Tet-based inducible shRNA or cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene...of pSLIK. Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression Ramalho-Santos...PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for CMV driven expression of cDNA. See ...pCW57.1 3rd Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions... -
Pushing the Limits of DNA Assembly
TypeBlog Post...Resources on the Addgene Blog Learn More about Gateway Cloning Gibson Assembly and Other Long-Homology... -
Plasmids 101: Blue-white Screening
TypeBlog Post...example is the ccdB gene system commonly used in Gateway® Cloning. There are also methods to select for ... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...part of our Genetic Pathway Reference Set. These Gateway-compatible entry clones are the product of many... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...amounts of cloning. Assembly is scarless, unlike Gateway cloning, and the method’s flexibility allows it... -
Microbiology Resources
TypeCollection... - Borodina Lab Golden GATEway cloning system - Wittbrodt Lab MultiSite Gateway Kit : Creating three-part...oleaginous yeast Y. lipolytica - Rossignol Lab Yeast Gateway kit : 285 vectors for expression, markers, fusion... -
27 Hot Plasmids from 2016
TypeBlog Post...74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0...The collection is compatible with both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are... -
Plasmids 101: Protein Expression
TypeBlog Post...of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein expression... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...collection is a set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, ... -
Anatomy of a Plasmid Page at Addgene
TypeBlog Post...may require strains such as ccdB survival (for Gateway plasmids). We also like to store plasmids that... -
Tetracycline Inducible Expression
TypeCollection...Lentiviral vector for inducible expression for Gateway cloning; selection cassette in format: PGK-rtTA...Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system for conditional cell line generation... Off Verma 26803 pEnt L1L3 EF1a-tTA-2 Contains Gateway L1L3 sites and EF1α promoter driving tTA; contains... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...Lahav G. Mol Syst Biol. 2014 Galit Lahav EYFP Gateway vectors with EYFP split at 175 Arabidopsis homolog... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog Post...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202 from Yu-Zhu... -
Tips for Using FRET in Your Experiments
TypeBlog Post...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest... -
Bacterial Expression Systems
TypeCollection...required. pET-HIS-Sangamo 40786 TALEN Gang Bao Gateway cloning and expression of an N-terminally His-tagged...endonuclease domain. pET-Sangamo-His 40787 TALEN Gang Bao Gateway cloning and expression of a C-terminally His-tagged...N-terminal 6x His tag and an MBP tag. Compatible with Gateway cloning. pDEST-periHisMBP 11086 Tac (lactose/IPTG...formation or prevent toxicity. Compatible with Gateway cloning. pGTvL1-SGC 39188 pTac (lactose/IPTG inducible...