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We narrowed to 42 results for: HRE-Luc reporter

Showing: 21 - 40 of 42 results

  1. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology... et al’s directed mutagenesis also produced the three separate timers shown in the table: Fast-FT, Medium-FT...lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells." Nature...

  2. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...in competition with other similar integrins, as reported by IPI and the Springer lab (Hao et al., 2024)...In this study, we, along with the Springer lab, reported the binding specificity, kinetics, and affinity...environment and the cytoskeleton. Most integrins have three conformational states: bent-closed, extended-closed...processes.   Figure 1: Integrins can shape into three conformations: (A) bent-closed, (B) extended-closed...cells to spread into symmetrical circles. While elucidating the role of tension, however, the researchers...individually and in combination, showing that among the three αV integrins expressed on the BJ-5ta fibroblast ...

  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014). This kit is a great starter...into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs...Multi-Kingdom Golden Gate Cloning Platform.” Scientific Reports, vol. 9, no. 1, Springer Science and Business Media...plant biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both ... to share it with the scientific community! Good luck and happy cloning! This article was written by Susanna...

  4. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...binding. Suzuki et al. also envision eNLs as good reporter genes for endogenous protein fusions created using...Renilla luciferase variant fused to an FP; when supplied with a luciferase substrate, the luciferase transfers...Nano-lantern, a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent technology is...Instead, a luciferase substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce...constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine. In addition...problems of low luminescence By itself, Renilla luciferase has a low quantum yield, and when transiently...transfer (BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...Arabidopsis, and corn. For both β-glucuronidase (GUS) and GFP reporters, as well as multiple transient and...to be removed and replaced if needed. In total, three MCS regions permit multiple regulatory elements ...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ...efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet, miniCMV Howard Gu...M2) with greater sensitivity to doxycycline and three copies of a minimal activation domain from VP16 ...pSBtet-GP Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al.,...

  7. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...-ome using a quick, affordable, and ubiquitous reporter assay. In their recent publication, Kroeze et ...endogenous proteins. As described in their recent Cell Reports paper, the Kanemaki lab overcame this hurdle using...TRIBE is the low input required - McMahon et al., report using as few as 150 fly neurons as their starting...delivery of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey...searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston...Drosophila cells, targets 13,501 fly genes with at least three independent gRNAs, each chosen strategically to ...

  8. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ... which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that ...G-protein-coupled receptors (GPCRs)! Aside from these three senses, GPCRs play roles in initiating signaling...transduction pathways and consist of a trimer of three subunits: alpha (Gα), beta (Gβ), and gamma (Gγ)....lab also assembled a collection of plasmids to elucidate the role of 14 of the 16 non-visual heterotrimeric...

  9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...buffered against the effects of copy number on reporter gene expression. On the other hand, using a constitutive...number in a cell. One can attempt to eliminate fluctuations due to copy number by introducing genes of interest...stabilized promoters perform during these types of fluctuations. When cells are dividing rapidly, multiple rounds..., the synthesis of deoxychromoviridans requires three genes whose proteins are needed sequentially. When...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...suitable for spatially limited studies and can fluctuate with changing culture conditions. The use of low...far-red/red light-responsive system is spread across three plasmids, with the response regulator encoded in...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds...two-photon microscopy to detect action potentials and subthreshold events, including miniature excitatory postsynaptic...deliver ASAP5 to the cortex and imaged voltage fluctuations in awake behaving mice through two-photon microscopy...

  12. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...Associate Professor at Boston College. National reports stress the importance of providing authentic research...complementation should only occur when galactose replaces glucose as the carbon source. Student results have shown...College. Students in the course work in teams of three in sections of 15 students. Within each section,...section, teams are assigned different groups of three genes selected from different parts of the Met pathway...

  13. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...Chris Humphrey from Jobs on Toast is based in the United Kingdom and scientists there would be lucky to ...

  14. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ..., as well as the Editorial Board of Scientific Reports and PLoS One. Additionally, you are involved in...as transcription and epigenetic regulation. To elucidate molecular mechanisms of regulation of genome functions...that in the US for me. In addition, I've been very lucky to work with excellent colleagues in my lab. On ...tendency is one of the reasons why we have only three biology-related Nobel prizes, out of which only ...

  15. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...transduced rat neurons in vivo with beta-gal and other reporters, and they showed that expression persisted for...The first lentiviral vector system consisted of three plasmids: the packaging, envelope, and transfer ...Fischer A, Cornetta K, Galacteros F, Beuzard Y, Gluckman E, Bushman F, Hacein-Bey-Abina S, Leboulch P. ...

  16. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...concept, they tested a lux-mediated bioluminescence reporter in vivo in the absence of selective pressure and... just described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin...Cara E., and James B. Kaper. "Use of stabilized luciferase-expressing plasmids to examine in vivo-induced...

  17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...colors (see table to the right); in various reports, Sanes and Lichtman have observed from 90-160 distinct...individual cells. The Brainbow-1.0 construct contains three fluorescent proteins: RFP (red), YFP (yellow) and...proteins, with recombination events specified by three loxP variants, but the principles remain the same...absent; stochastic Cre excision results in one of three recombinations, and subsequent recombinations cannot...recombination events leads to a rainbow of colors. With three copies of the construct, one would expect ten colors...) From the seven resulting proteins, they chose three with low levels of both fluorescence and sequence...Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835...

  18. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ..., et al. "One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated...Easi-CRISPR works best with insertions of 2 kb or less. Luckily, most of the commonly used cassettes, such as fluorescent...Quadros et al. saw success with Easi-CRISPR across three independent core facilities for 13 projects, with...

  19. Chemogenetics Guide

    Type
    Guide
    ...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific...light-emitting luciferase and a light-sensing optogenetic element. When the luciferase substrate (luciferin) is ...efficiencies. Depending on the type of luciferase used, the luciferin substrate may be the native coelenterazine...in neurons LMO Luciferase Opsin Ligand Effect Outcome (in neurons) Reference LMO3 sbGLuc VChR1 CTZ Na +... activation Slaviero et al., 2024 iLMO: RLuc-NpHR Venus-Rluc8 NpHR hCTZ Cl - influx Neuronal inhibition...intracellular calcium stores. There are currently three Gq DREADDs based on human muscarinic receptors: ...is added, the luciferase enzyme generates light that activates the opsin. The opsin may excite or inhibit...

  20. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...β-like-cells [37, 38]. In 1987, Davis et al. reported one of the earliest examples of transdifferentiation...from fibroblasts, human keratinocytes from hair pluck, peripheral blood cells, and renal epithelial cells... damage results in the transdifferentiation of glucagon-producing α-cells into insulin-producing β-like-cells...Dedifferentiation, transdifferentiation and reprogramming: three routes to regeneration. Nat Rev Mol Cell Biol, 2011...
Showing: 21 - 40 of 42 results