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We narrowed to 319 results for: INO

Showing: 21 - 40 of 319 results

  1. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...1350 amino acids in size, which includes SpCas9, and shortern orthologs approximately 1000 amino acids..., only Staphylococcus aureus Cas9 (SaCas9, 1053 amino acids) displayed cleavage activity in mammalian ...that’s small enough to package into AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni ... Neisseria meningitidis Cas9 (NmeCas9) at 1,082 amino acids, can also be packaged into AAV. NmeCas9 has...these Cas proteins with AAV, at just 1,108 and 986 amino acids, respectively, the size of both is within ...

  2. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...component of the nucleoside inosine. Created with BioRender.com. Inosine excision leads to the first...it to act on newly-deaminated inosines. Pro tip! The nucleoside inosine consists of the nucleobase hypoxanthine...repair. But mammals also have an enzyme to remove inosine from DNA: N-methylpurine DNA glycosylase (MPG),...extends to thymine Since an enzyme that excises inosine can be modified to target guanosine instead, perhaps...

  3. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...for longer. Cultures induced with arabinose and colonies on arabinose-containing plates will be visibly... inducible expression systems in E. coli. The arabinose-inducible promoter pBAD and a low-copy number ...wide range of inducer concentrations (0.1-1% L-arabinose) without signs of toxicity or burden relative ...or on LB-agar plates containing glucose and L-arabinose at 37oC for at least 12 hours, or at lower temperatures...after the E. coli have colonized tumors, inject L-arabinose intraperitoneally and wait 24 hours before imaging...

  4. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ... Balla. "Live cell imaging of phosphoinositides with expressed inositide binding protein domains." Methods...be downsized by deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about... but the development of non-natural fluorescent amino acids and their incorporation into proteins are ...homology domains can be used to detect specific phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production...10. Hilaire, Mary Rose, et al. "Blue fluorescent amino acid for biological spectroscopy and microscopy....

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...Marketing Manager at sciNote. Tea Pavlek is a Product Marketing Manager at SciNote, interested in combining...research with tech advances. You an follow SciNote on Twitter @scinoteELN Additional Resources on the Addgene...blogger, Tea Pavlek, Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches...priorities for future ELN development and developed the SciNote Electronic Lab Notebook. Implementing an electronic...

  6. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ...critical amino acid). Many common E. coli strains used for cloning are auxotrophic for specific amino acids...bacteria (one that doesn’t synthesize an essential amino acid), and a plasmid that complements the auxotrophy...acids. When plated on media lacking the essential amino acid, the bacteria will only grow if it acquired...containing the gene necessary to synthesize the missing amino acid. Auxotrophic selection is often used for cloning...

  7. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...mis-translation Bactericidal 50-100 ug/mL Spectinomycin aminoglycoside Binds 30S ribosomal subunit; interrupts...Action*   Working Concentration** Kanamycin aminoglycoside Binds 30S ribosomal subunit; causes mis-translation... Bactericidal 7.5-50 ug/mL Streptomycin aminoglycoside Inhibits initiation of protein synthesis ...macrolide Blocks 50S ribosomal subunit; inhibits aminoacyl translocation Bacteriostatic 50-100 ug/mL in...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...result in a spectinomycin resistant phenotype in bacteria. While there are many known spectinomycin resistance...increase the mutation rate of EvolvR, three additional amino acid changes were introduced to nCas9. These changes...five dispersed regions of the target gene of spectinomycin, rpsE. These results not only uncovered novel...used to generate bacteria resistant to both spectinomycin and streptomycin, but could also be used for...

  9. Video Library

    Type
    Protocol
    ...efficiency Bacterial Transformation Protocol Inoculating a Liquid Bacterial Culture How to prepare and...and grow bacteria in a liquid medium Inoculating a Liquid Bacterial Culture Protocol Getting Started with...

  10. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...Acinetobacter calcoaceticus, Actinobacillus pleuropneumoniae, Actinomyces naeslundii, A. viscosus, Aerobacter...Providencia spp., Pseudomonas spp., Rhizobium leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus...Providencia stuartii, Pseudomonas spp., Rhizobium leguminosarum, R. trifolii, Salmonella enteritidis, S. typhimurium...fluorescens, P. putida, Rhizobium meliloti, R. leguminosarum by. viciae, Rhodobacter sphaeroides, Salmonella...

  11. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...One of the limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have...multiple cloning site and therefore consist of random amino acid sequences or (ii) designed to form an inert...glycines, serines and other small, non-aliphatic amino acids. However, long linkers increase the chance.... To indicate the sequence, people often use terminology like “C-terminal fusion,” but I find this very...protein fusions, our experience is that a linker of 4 amino acids (PVAT, when we use the AgeI site in the multiple...

  12. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...associated with phosphorylation. However, these amino acids fail to recapitulate the true steric and charge-based... version of GFP will contain a phosphoserine at amino acid 17 instead of a glutamate which allows the ... of these expression vectors, the codon for the amino acid to be replaced with phosphoserine must be recoded... a phosphoserine) to control for effects of the amino acid substitution. Alternatively, you can express..."Cysteinyl‐tRNA formation: the last puzzle of aminoacyl‐tRNA synthesis." FEBS letters 462.3 (1999): 302...

  13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...IMP organizers Rosario F. Godino, Rocío López-Diego, & Zafira Castano Corsino. When someone says “internationalization... developed by its Director, Dr. Zafira Castano Corsino, a junior faculty member at Harvard Medical School...International Mentorship Program Zafira Castaño Corsino is a Junior Faculty at Harvard Medical School/BWH...zcastan or on linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical...

  14. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ...listed as the amino acid change and not a nucleotide change. The proper way to denote an amino acid mutation...list the one letter abbreviation of the wild type amino acid immediately followed by its position (number...followed by the one letter abbreviation of the mutated amino acid currently at that position. In contrast, unmutated...

  15. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...letters of the DNA get translated into the twenty amino acids. Once scientists established that DNA encoded...meganucleases. The storyline involves the resurrection of dinosaurs by extracting the DNA from fossilized amber and...the insatiable appetite of the moviegoers for dinosaurs wreaking havoc in a theme park.  Released in 1997...ideal. For the early generation gene editors, new amino acid recognition sites had to be designed each time...the movie involves The Rock and his super giant albino gorilla fighting off a monstrous wolf and crocodile...

  16. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Tsien’s lab in 1998 (Griffin et al., 1998), this 15 amino acid peptide contains a cluster of four cysteines...cysteines (CCXXCC, where X is any non-cysteine amino acid) that reacts with biarsenical dyes such as FlAsH (...on your protein of interest or using non-natural amino acids to directly incorporate a fluorophore in your...popular self-labeling tag systems Tag Size (amino acids) Ligand Commercial ligands available ...varies Various Few Complicated Non-natural amino acids 1 Various Many Complicated   ...

  17. NETRF

    Type
    Collection
    ...discover cures and more effective treatments for carcinoid, pancreatic, and related neuroendocrine cancers... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University Epigenetic... Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute Identifying...Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  18. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Lercher, Spicer, & Davis, 2015). By using a novel aminoacyl tRNA synthetase/tRNA pair, a researcher can lure...the ribosome into inserting a fluorophore-tagged amino acid at a stop codon (often amber i.e. UAG) engineered...site. During translation, the novel tRNA/unnatural amino acid finds the UAG codon in the ribosome, resulting...Synthetase Expression Host pMAH-POLY Polyspecific aminoacyl-tRNA Synthetase Mammalian Cells pDULE-ABK pyrrolysyl-tRNA...pyrrolysyl-tRNA synthase for aliphatic diazirine amino acids E. coli and Mammalian Cells pEVOL-pAzF synthetase...

  19. Zhang Lab CRISPR Page

    Type
    Collection
    ... Lin CY, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F. Cell ....top three significantly mutated genes in lung adenocarcinoma. Delivery of a single AAV vector ( AAV-KPL ...mutations, leading to macroscopic tumors of adenocarcinoma pathology. These plasmids as well as a backbone...CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...are problematic because they require radiolabeled amino acids. The use of radioactive materials is highly...applies metabolic labeling using a noncanonical amino acid, azidohomoalanine (AHA). In the technique, ... labeling is known as fluorescence noncanonical amino acid tagging (FUNCAT, Dieterich et al. Nature Neuroscience...B., and L. Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings of...
Showing: 21 - 40 of 319 results