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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 298 results for: IRES

Showing: 21 - 40 of 298 results

  1. Viral Production at Addgene

    Type
    Blog Post
    ...cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral genomes (...expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral...

  2. New Viral Vectors - March 2024

    Type
    Blog Post
    ...Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik New viral vector...

  3. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...doi.org/10.1128/jvi.71.8.6174-6178.1997 Wickersham IR, Finke S, Conzelmann K-K, Callaway EM (2006) Retrograde...–49 . https://doi.org/10.1038/nmeth999 Wickersham IR, Lyon DC, Barnard RJO, Mori T, Finke S, Conzelmann...Infection of these cells with VSV-G pseudotyped virus requires the upregulation of LDLR expression using other... system (Wickersham et al., 2007). This system requires first supplying rabies glycoprotein along with...

  4. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 5 Yizhar 109048 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST CAG eGtACR1 (soma-targeted) mRuby3 Cre...

  5. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...be separated by an internal ribosome entry site (IRES) and expressed under the same promoter. Alternatively...cells - one containing the gene of interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA...in terms of RCL generation because this vector requires only three HIV-1 genes (gag, pol, and rev) for...that there is often  background activity and it requires continuous administration of Dox to repress transgene...One drawback of the Tet system is that it often requires the delivery of two distinct expression vectors...

  8. Tetracycline Inducible Expression

    Type
    Collection
    ... or Off) None Tight TRE Markus Ralser 64238 pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression...

  13. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...74(5):858-73. Pubmed PMID 22681690.  8, Wickersham IR, Finke S, Conzelmann KK, Callaway EM. Retrograde ...

  14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...interest, but after an internal ribosome entry site (IRES). This is oftentimes used in conjuction with FACS...co-factors, substrates, or enzymatic activity – it only requires the presence of oxygen during maturation. This...

  17. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... vector, contains a promoterless splice acceptor-IRES-neoR gene, which enhances the efficiency of homologous...experiment. For this reason, the AAV-based approach requires G418 selection of infected clones, making it unsuitable...

  18. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ... fluorescent protein downstream, separated by an IRES element. You did a lot of work! But let’s take a...-thi-1 or thi1 Mutation in thiamine metabolism Requires exogenous thiamine for growth ara disruption...leuB β-isopropyl malate dehydrogenase inactivated Requires exogenous leucine source for growth proAB mutation...mutation in proline biosynthesis pathway Requires exogenous proline source for growth rpsL Mutation ...

  19. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian U6 yes, cut S. pyogenes Kuehn pMCS-rybozyme-IRES-CAS9 64668 Mammalian yes, cut S. pyogenes W Fujii...Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires that you have to express both a Cas protein and...expression of multipe gRNAs. Csy4 cleavable cassettes; requires expression of Csy4 (eg Plasmid 53369 ). Joung ...
Showing: 21 - 40 of 298 results