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We narrowed to 1,039 results for: LAS

Showing: 21 - 40 of 1039 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...articles provide a synopsis of a plasmid or group of plasmids' functions and applications. We hope that... you to find and use the plasmids you need. You can find all the hot plasmids from 2017 below. With over...over 50,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...the growing list of popular plasmids in Addgene’s FP collection. Just last month, Robert Campbell’s lab...editing for precise genome modification. Plasmid ID Plasmid Name Cas9 Variant Speacial Features 85169...

  2. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...conjugative mobilization of broad host-range IncQ plasmids." Plasmid 62.2 (2009): 57-70. PubMed PMID: 19465049...page Deposit microbiology plasmids with Addgene Find synthetic biology plasmids ...family-RP4 conjugation system. This system can deliver plasmids to both Gram-positive and Gram-negative cells.... Wang lab further developed a modular suite of plasmids (pGT) with various replicative origins that can...genera across four phyla that have taken up the plasmid through conjugation in vivo studies. However, they... tools for microbiome studies.  Find the MAGIC plasmids here Paper-based synthetic biology approach to...conjugation. The labs screened for the host-range of the plasmids and suitability of various antibiotic resistance...

  3. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...depositors. Below are the 15 top requested plasmids over the last 15 years - the majority of these being ...times have these plasmids been shared in total? Just over 49,000 times! Their long-lasting popularity illustrates...and an envelope expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find...wide range of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB... top requested plasmid from 2016. You may notice the “V2.0” at the end of this plasmid which indicates...prior version of this plasmid. Indeed the first version is the 15th most popular plasmid of all time, but ...envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini...

  4. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...consider your plasmid's compatibility with one or more other plasmids. Generally speaking, plasmids with the...enabling a plasmid to reproduce itself as it must to survive within cells. The replicons of plasmids are generally...cell. Figure 1: A plasmid map showing the standard features of a plasmid. So, how do you choose...have only one plasmid type in your cells at a time? Do you want to make a lot of your plasmid? Is the gene...avoid plasmid incompatibility In other words, the best choice of ori depends on how many plasmid copies...a rule, plasmids from the same group should not be co-transformed, so if you need two plasmids for an ...purify your plasmid DNA: The insert Bacteria tend to maintain fewer copies of plasmids if they contain...

  5. Hot Plasmids - October 2020

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...long lasting and was shown to have a half life of 84 hours in primary mouse embryonic fibroblasts. This...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...head over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively...offers advantages for cellular delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered...

  6. Hot Plasmids - August 2020

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... cells were transfected with plasmids encoding FPs tagged with the plasma membrane targetin sequence derived...They generated plasmids tagged with EB3 to mark the growing tips of microtubuli and plasmids tagged with ...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...and dCas9-VPR targeting plasmids can be found at Addgene. Check out these plasmids for piggyBac gRNA delivery...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...

  7. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here! References...antibiotic resistance markers (indicated here by plasmid color), allowing you to easily select for the expression...desired, recombined construct. Read our recent Plasmids 101 Post on CcdB for more information. How to ...Method A: recombination of an attB-PCR product or plasmid with an attP donor vector. In this case, we would... to use the Snapgene program to design Gateway plasmids.    Figure 3: Method A to create an entry...entry clones can be used with a large variety of plasmids. Speed The Gateway system enables the generation.... Take a look at some of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...apicomplexan organism Toxoplasma gondii to the infection of human fibroblasts. Toxoplasma mutants were generated...Neurosci 2016. PubMed PMID: 26950006 Plasmid ID Plasmid Name Plasmid Type 74309 pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief articles...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ... you to find and use the plasmids you need. You can find all the hot plasmids from 2016 below. With over...over 45,000 plasmids, we can't write posts for every great plasmid that comes into the repository, but...write about your plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the...

  9. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Tet-Off system.  For more information on Addgene Tet plasmids, see our Tetracycline resource page. Cumate Switch...20697123. PubMed Central PMCID: PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez...20434990. PubMed Central PMCID: PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, ...22406855. PubMed Central PMCID: PMC3846601. Find plasmids from this publication at Addgene. Riabinina, ...25581800. PubMed Central PMCID: PMC4344399. Find plasmids from this publication at Addgene. Riabinina O...Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible Drosophila...

  11. Hot Plasmids - October 2022

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...post: Improved voltage indicator: JEDI-2P Novel class of light-gated potassium channelrhodopsins Myc-tag... you can also find several other constructs in plasmid form. Fig. 1: Summary of the approach and...JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue.../doi.org/10.1016/j.cell.2022.07.013.   Novel class of light-gated potassium channelrhodopsins by: Mike...Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins, named...

  12. Hot Plasmids - May 2022

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here. Here's what you'...easily be applied to other systems.    Find BLITZ Plasmids here! Xiong, et al. eLife. 2021. https://doi.org...from Goertsen D, et al., 2022.  Find M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience....have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and microbial...Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol. 2022. https:/...

  13. Hot Plasmids - February 2022

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'...ability to knock out multiple genes in parallel. Lastly, this Cas12a-based screening method was used to...Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective alternative... ladders. Image from Santilli, et al. 2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60...kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also available...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited strain carrying... or want to swap out the constant domains, the plasmids for the heavy chain and light chain are also available...ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova, A. et al. (2024). Design of ...minimal background.   Find these C. elegans mScarlet plasmids here!   Cao W.X., Merritt D.M., Pe K., Cesar ...disruption in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system By Aryana Khosravani Deborah...Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium...

  15. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. ...be used for applications beyond cryo-EM.   Find plasmids for MagIC-Cryo-EM here!   Arimura, Y., Konishi...disease phenotype in an animal model. Find PE-eVLP plasmids here! The lab’s other recent deposit builds on...over the wild-type enzyme. Find optimized PASSIGE plasmids here!     Figure 3: Schematic of the PASSIGE...recombinase integrates a sequence from a donor plasmid containing a complementary site into the genome...fused to a DNA methyltransferase — that can drive lasting transcriptional silencing (Nuñez et al., 2021)....expression off. Plus, DNA methylation is a long-lasting repressive marker, allowing the team to develop...

  16. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...factors impacting prime editing and found the protein La (a small RNA-binding exonuclease protection factor...They developed a new prime editor that incorporates La’s N-terminal domain, PE7 (Figure 2).  PE7 shows enhanced...the improved efficiency is at least in part due to La binding and stabilizing the 3’ end of the pegRNA,...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime...

  17. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...future improvements. Find PinkyCaMP plasmids and FRCaMPi plasmids! Fink, R., Imai, S., et al. (2024).... fat cells fight cancer Frugal CRISPR kit for classrooms Cell-surface proteomics for neurons and astrocytes...isolated, identified by mass spectrometry, and classified by source cell based on single-cell RNA-seq data...cell types, and tissues. Find cell-surface HRP plasmids here! Wu, L., et al. (2025) The cell-surface ...systems, both in vitro and in vivo. Find MitoTRACER plasmids here! Borcherding, N., & Brestoff, J. R. (2023... al. (2025) under CC BY license. With 143 plasmids deposited from this paper, a large menu of tool...

  18. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...applications. Find ATLAS plasmids here! Rivera, J. F., Huang, H., et al. (2025). ATLAS: a rationally designed...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles... and AAV11 packaging plasmids Mapping anterograde synaptic connections with ATLAS Bright and photostable...Find Addgene’s AAV6 and AAV11 packaging plasmids here!   An ATLAS for anterograde synaptic connections ...fluorescent protein mGold2 By Mike Lacy Get your sunglasses — these proteins are bright! Most current yellow...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright... to the broad host range of TRV. Find TRV-TnpB plasmids here! Weiss, T., et al. (2025). Viral delivery...

  19. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins...and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! ... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything... swap sequences effortlessly.  We built these plasmids for flexibility and speed, so you can design, ...previous integrase-based approaches. Find STITCHR plasmids here! Fell, C. W., Villiger, L., Lim, J., Hiraizumi...

  20. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... Resistance Gene Plasmids 101: Yeast Vectors Plasmids 101: Mammalian Vectors Plasmids 101: The Promoter...other plasmids? If we all use the same molecular code, can I transfect any old bacterial plasmid into ...blog Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection Plasmids 101:..., where DNA replication begins, so a bacterial plasmid needs a compatible ori that will be recognized ...prokaryotic growth. Since there are no natural mammalian plasmids, mammalian vectors are even less similar to bacterial...transfection. In stable transfection, DNA from the plasmid is incorporated into the host cell’s genome, so...duration of the experiment Selection Once a plasmid is delivered to your target cells, you still need...
Showing: 21 - 40 of 1039 results