We narrowed to 82 results for: LucY

1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   ...number of functions, including: tagging genes for elucidating their expression or localization profiles, acting...application Almost as soon as its sequence was elucidated, scientists began engineering new versions of...

2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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   ...copies per microliter) viral load in the saliva (Lucia et al., 2020). Detecting coronavirus using CRISPR-based...3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico, P.-B., & Alejandra, G. C. (2020)....

3. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   ...proteins is hard to imagine yet alone study. To elucidate these interactions scientists have long used antibody...Research Techniques Made Simple: Emerging Methods to Elucidate Protein Interactions through Spatial Proximity...

4. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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   ...overcome the limitations posed by autofluorescence (Lucidi et al., 2023). The field of fluorescent ligands...64–72. https://doi.org/10.1007/s41048-017-0037-8 Lucidi, M., Capecchi, G., Visaggio, D., Gasperi, T., Parisi...

5. 15 Hot Plasmids from 2017

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   ...subtilis libraries Transcription factor fusions elucidate iPSC reprogramming mechanism The Ptashne lab ... al., they used this now classic technique to elucidate the mechanism by which SOX2 acts to reprogram ...

6. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

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   ...Bengyella Dr. Bengyella’s work has focused on elucidating the molecular mechanisms behind interactions ...

7. Fluorescent Proteins 101: History of Fluorescent Proteins

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   ...light include chemiluminescent proteins, like luciferases, as well as fluorescent ones, like Green Fluorescent...

8. SunTag and Fluorescent Imaging

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   ...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

9. Plasmids 101: What is a plasmid?

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   ...Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of a chemical in a particular...

10. Understanding GPCR Signaling Using TRUPATH

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    ...biosensor system was developed by constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ...

11. Advice for Choosing a Research Project

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    ...selective, and talking to peers and mentors will help elucidate which project is right for you.   Acknowledgments...

12. History of CRISPR Cas - A tale of survival and evolution

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    ...nucleases, and helicases, were also disrupted to elucidate their role in the process. The results of these...

13. Antibodies 101: Epitope Availability

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    ...troubleshooting immunoimaging assays. It can also elucidate information about what a given protein is doing...

14. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    .... In their assay, the pseudoviruses express a luciferase reporter which is used as a readout for infection...

15. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    ...Addgene, we’ll be able to make big progress in elucidating association between SOX10 and WS4 and also several...

16. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    ...ApoB-NanoLuc reporter by inserting the NanoLuc luciferase downstream of the apoB gene. Using this reporter...

17. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    ...mechanisms of miRNA regulation have yet to be fully elucidated. Ron Weiss’s lab at MIT pushed the field further...

18. Working with Nuclear Receptors

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    ...evolved from chloramphenicol acetyltransferase to luciferase, the basic assay remains widely used today.  ...

19. 5 Steps to Writing Grant Proposals for Grad Students

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    ...technical writing and communication skills, Elucidates the big picture and future directions of the ...

20. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post

    ...especially true in the case of protein tagging to elucidate cellular structures. Overexpression methods flood...