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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 163 results for: NAP

Showing: 21 - 40 of 163 results

  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...promoters for general RNAP II and RNAP III transcription; however, using viral LTRs as RNAP II promoters is ...to use an RNAP II promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP III promoters...is transcribed from DNA using an RNA polymerase (RNAP). In bacteria this is done by a single enzyme; however...of RNAs. To gain this specificity, the eukaryotic RNAP can recognize and bind to specific promoter elements...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...A122D WPRE (AAV1) pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9...signal to noise ratio and is better localized for synaptic glutamate response. They generated variants optimized...activation kinetics and localization for imaging synaptic transmission. bioRxiv 2022.02.13.480251. doi: ...AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes...the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1) pGP-AAV-syn-jGCaMP8f-WPRE...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex, while...ideal for video recordings of large populations of synapses and deep-brain fiber photometry.  Figure...promise to be a popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids ...with tools like mass spectrometry, providing a snapshot of interacting proteins at a certain time and ...

  4. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    .../B), which cleaves the synaptic protein VAMP2, a protein essential for synaptic transmission. Cleavage... Hot Plasmids podcast Light-mediated block of synaptic transmission Article contributed by Klaus Wanisch...optogenetic tool that allows light-mediated silencing of synapses was developed by Matthew J. Kennedy’s and Chandra...demonstrated the utility of light-mediated suppression of synaptic transmission in brain slices from mouse, and showed...

  5. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ...Many thanks to our guest blogger Erik L. Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology...This post was contributed by guest blogger Erik L. Snapp. Stop using EGFP/GFP for fusion proteins! Despite...distance runner, gardner, and cook.   References 1. Snapp, E.L., et al., Formation of stacked ER cisternae... Resources on the Addgene Blog Read Professor Snapp's Post on mOX FPs Find the Best Fluorescent Protein...

  6. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...individual presynaptic terminals, which are jam packed with everything needed for synaptic transmission...it difficult to study the inner workings of the synapse by conventional light or fluorescence microscopy...researchers gain a deeper understanding of neuronal synapses, centrosomes, parasites, and more (Bertiaux, et... al (2010) Superresolution Imaging of Chemical Synapses in the Brain. Neuron 68:843–856. https://doi.org...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...fragments, expressed in the presynaptic region of one neuron and postsynaptic region of a different neuron...Currently available from the Kim lab are 2 presynaptic and 2 postsynaptic targeting mGRASP plasmids. Additionally...efforts to map the location and distribution of synapses in the mouse brain. Kim et al is utilizing a mammalian... mammalian GRASP (GFP reconstitution across synaptic partners) technique based on functional complementation...neuron, come into proximity in the synaptic cleft, functional fluorescent GFP is reconstituted in vivo. ...

  8. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...  Erik Snapp just released version 3.4 of his eBook, How to Get a Faculty Position. This edition has ...along with new thoughts on research statements. Snapp, who is always looking for ways to improve his eBook...suggestions, please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog... Sides," are summarized below. Check Out Erik Snapp's Full "Applying for a Faculty Position" eBook Here... Skye). Good luck with your search!   Erik Lee Snapp received his PhD in Molecular Microbiology and Immunology...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...axonal transport  (Murlidharan et al., 2014), and postsynaptic transmission (Oh et al., 2020). For example,...Others, like AAV1 and AAV9, can also transduce postsynaptic cells when used at very high titers (Zingg et...Selective promoters Selective promoters, such as synapsin, glial fibrillary associated protein (GFAP), or...Rah, J. C., & Park, H. (2020). Labeling dual presynaptic inputs using cFork anterograde tracing system...Zhang, L. I. (2017). AAV-Mediated Anterograde Transsynaptic Tagging: Mapping Corticocollicular Input-Defined...

  10. New Viral Vectors - March 2024

    Type
    Blog Post
    ... pAAV-hSynapsin1-RdLight1 AAV9 Biosensor Tian New viral vector pAAV_hSynapsin_psychLight2 AAV9 Biosensor...lab. Also of interest is the Tian lab's pAAV_hSynapsin_psychLight2 (AAV9), which contains a biosensor that...Biosensor Tian New viral vector pAAV.hSynapsin.SF-iGluSnFR.A184S AAV9 Biosensor Looger New serotype ...

  11. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...WPRE 111262  AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3... Plasmid Serotype Name 106176  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid...axon-GCaMP6s-P2A-mRuby3) 112010  AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name ...

  13. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...that are intricately wired into circuits through synaptic connections (Shepherd, 2004). Understanding the...something researchers often want to do at neuronal synapses for instance. Also these tags need to be fused...proteins. For example, N-cadherin (cadherin-2) is a postsynaptic cell adhesion protein that plays a critical ... PMC4573404. 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University ...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...Young JAT, Callaway EM (2007) Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically... can travel in a retrograde direction from the synapse to the cell body, making it a great tool to study...virus envelope, travel retrogradely, and jump the synapse to the previous neuron (Wichersham et al., 2007...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...to mCherry, all under the control of the human synapsin promoter. Purpose of this AAV pAAV-hSyn-DIO-hM4D...restricted to a particular cell type. The human synapsin promoter further specifies neuron-specific expression...PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers highly neuron-specific...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    ...deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature” library...

  18. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...involved in neurogenesis, neuronal survival, and synaptic plasticity. Several studies have found reduced...stimulation to emulate optogenetic treatment of synaptic pathology." Science 347.6222 (2015): 659-664. ... Van Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic system in ...

  19. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs... Neuron-specific expression is mediated by the synapsin 1 (SYN) promoter, which was superior in its ability...physiology by promoting the development of neurons and synapses as well as long-term memory formation. The Bdnf...

  20. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ... Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...
Showing: 21 - 40 of 163 results