Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 130 results for: NAP

Showing: 1 - 20 of 130 results

  1. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...powered by GSL Biotech's SnapGene Server Software. With the backing of SnapGene’s sequence viewer software... Overview of SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid...the features you need quickly. SnapGene’s Feature Library - SnapGene’s software seamlessly identifies ...example below. Our old map is on the left while the SnapGene powered map is on the right (click here to see...t detected by our in-house software. In fact, SnapGene’s feature library includes nearly 3x as many features...identify. Improved Restriction Enzyme Handling - SnapGene’s software positions restriction enzyme annotations...you can download an annotated GenBank File or a SnapGene file for the sequences listed. You can then analyze...

  3. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ... who uses SnapGene or the free SnapGene Viewer companion product. If you don’t use SnapGene yet, you can...guest bloggers Aline and Benjamin Glick from SnapGene. SnapGene was created to meet a need. While there were...approach is SnapGene’s algorithm for detecting common features. This algorithm enables one of SnapGene’s most... us at snapgene.com. Many thanks to our guest bloggers Aline and Benjamin Glick from SnapGene!  Ben Glick...Addgene Blog Read about our SnapGene powered plasmid maps Learn how we use SnapGene during the plasmid quality...properties of the DNA molecules they were using. SnapGene was created to alleviate these problems through...make specialized controls available when needed. SnapGene has been engineered to be easy and enjoyable to...

  4. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    Published
    Feb. 1, 2018, 3:07 p.m.
    ...through our Snapgene powered maps and sequence analysis tools.     The main ways we use SnapGene for plasmid...version of Snapgene to visualize the sequence and look for features. The first thing that the Snapgene software...proteins that are already included in Snapgene’s extensive database. Snapgene also provides easy visualization...software as the desktop version of Snapgene. When you download a Snapgene file directly from our website,...website, you can open it with either the Snapgene viewer or Snapgene Desktop programs to analyze the sequence... plasmids. We’ve also talked about how our new Snapgene generated maps provide improved feature detection...with an easy to use interface. We regularly use Snapgene for our quality control process because of its...

  5. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ... Keep your kids on a schedule so you can rely on nap times, after bedtimes, or before they wake up for...

  6. Rabies and Neuronal Tracing

    Type
    Blog Post
    Published
    May 29, 2018, 1:51 p.m.
    ...transmitted to adjacent neurons via synapses (i.e., transmitted transsynaptically). This is because the recombinant...Rabies Virus: Converting a transsynaptic neuronal tracer into a monosynaptic (first order) neuronal tracer... able to replicate, and for a virus to cross a synapse from one cell to another, it must replicate. Modified...replicates within that cell, and nascent RABV cross synapses to retrogradely infect neurons that project to...higher order neural connections. While this transsynaptic spread is useful for neuronal tracing, it has...starter cell types, as well as to only spread monosynaptically from these cells. These modifications take...express EnvA unless it is supplied in trans. Monosynaptic RABV spread can be achieved by modifying the...

  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...retrograde labelling, synaptic restriction as well as controlled monosynaptic and polysynaptic spreading. AAV... AAV9 are capable of synaptic spread. The underlying mechanisms of trans-synaptic trafficking are not ...., and Callaway, E.M. (2007). Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically...sending information onwards to other neuronal cells. Synapses at the terminals of axons form connections to ...the prefrontal cortex, but also indirectly via synapses to neurons in other brain regions. Further, the...tracing compounds is that their diffusion across the synaptic cleft is usually severely impaired, and only few... are transported from one neuron to another at synapses resulting in faint signals (Schwab et al., 1979...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR: The Virginia...fluorescein) or ReAsH (red, based on resorufin). SNAP-tag: In 2003, Kai Johnsson’s lab developed a self-labeling...2008). Although based on the same original enzyme, SNAP-tag and CLIP-tag show good specificity for their...Biarsenical dyes (FlAsH, ReAsH) Few Easy SNAP-tag 182 Benzylguanine (BG) Many Easy ...often up to 10-fold more). So, labeling a HaloTag or SNAP-tag construct can help you better detect dim structures...magenta) and outer mitochondrial membrane labeled with SNAP-OMP25 with Atto590-BG (green), imaged with stimulated...yellow) at the tip. 649-mDia1 and 549-CP are both SNAP-tag fusions, purified and labeled separately with...

  9. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...found at excitatory synapses (Rivera & Huang, et al., 2025). AF was fused to a presynaptic vesicle protein...remain on the presynaptic cell membrane while AF, fused to a payload, is released into the synapse. Once AF...and AAV11 packaging plasmids Mapping anterograde synaptic connections with ATLAS Bright and photostable...nucleus, endosomes, lysosome, golgi, ER, inhibitory synapses, and cytoskeleton components) and in a FRET-based...packaging plasmids here!   An ATLAS for anterograde synaptic connections By Emily P. Bentley While several ...lack of genetic control. ATLAS (anterograde transsynaptic label based on antibody-like sensors) changes...AF binds to the postsynaptic AMPA receptor and is endocytosed, the Cre recombinase payload activates a...

  10. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...neurons and their synapses.   How can you label a single neuron and its synapses in the living brain...cytoplasmic markers (e.g. eYFP) and synaptic proteins (e.g. postsynaptic PSD95-mcherry) are used in high ... a single cell with all its synapses and how it rearranges these synapses in the living brain over time...mix to label neuronal morphology (eYFP) and the synaptic protein PSD95 (PSD95-mcherry) under the control... if the cellular structure of a neuron and its synapse should be visualized at the same time from two ...Expression of a morphological marker (eYFP) and synaptic marker (PSD95-mcherry), under the control of Flp...CreERT2), visualizes individual neurons and their synapses in a conditional knockout mouse. Tamoxifen injection...

  11. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...Cre-expressing cells hSynap: Human Synapsin-1 promoter. Good for neuronal expression. hSynap-FLEX: Synapsin-1 promoter...and accurately differentiating between synaptic vs. extrasynaptic glutamate release. Building on the previous... for expression in Cre-expressing cells. hSynap-FLP: Synapsin-1 promoter, FLP-dependent expression GFAP...in vivo. Glutamate plays a variety of roles in synaptic communication and can trigger other forms of neuronal.... iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations of ...that are more sensitive and better localized for synaptic glutamate response than its predecessors. The ...activation kinetics and localiation for imaging synaptic transmission” bioRxiv (2022)...

  12. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... the synaptic scaffolding proteins PSD95 and gephyrin to label excitatory and inhibitory synapses, respectively...bind to the endogenous synaptic proteins allowing them to localize to the synapse and minimize extraneous...inhibitory or excitatory synapses or that are Cre-inducible, researchers can explore synaptic integration and ...post: Tools for labeling excitatory inhibitory synaptic proteins Fluorescent protein system for C. elegans... service Labeling excitatory and inhibitory synaptic proteins with FingRs By Jason Nasse Since the ...billion neurons in the human brain. But studying synaptic connections and physiology is particularly challenging...selectively visualize excitatory and inhibitory synapses in the brain.  Figure 1:Schematic diagram...

  13. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...Figure 2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein...doi.org/10.1101/2024.02.11.579803).  CLIP, CLAP, SNAP! Self-labeling tags for single-molecule biophysics...Twelvetrees Lab took advantage of the self-labeling tags SNAPf and CLIPf. These tags rely on synthetic dye molecules...combining an N-terminal CLIPf tag and a C-terminal SNAPf tag; see Figure 2), the authors developed a cost-effective...KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-tagged proteins for mammalian...other proteins of interest too! Find CLIP, CLAP, and SNAP plasmids here! Smith, E.R., Turner, E.D., Abdelhamid...indicator with enhanced sensitivity for unitary synaptic events By Brian O’Neill The Lin Lab and collaborators...

  14. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...our guest bloggers Erik L. Snapp and Lindsey M. Costantini! Erik Lee Snapp received his PhD in Molecular... post was contributed by guest bloggers Erik L. Snapp and Lindsey M. Costantini. "You underestimate the...have reported that FP cysteine residues form inappropriate disulfide bonds in the secretory pathway (Figure...localization. For example, the formation of inappropriate inter-chain disulfide bonds between FPs blocks...research was conducted in the laboratory of Erik Lee Snapp and focused on optimizing fluorescent proteins for...sarcoma-associated herpesvirus.       References 1. Snapp, Erik. "Design and use of fluorescent fusion proteins... 18228466. Pubmed Central PMCID: PMC2875081. 2. Snapp, Erik Lee. "Fluorescent proteins: a cell biologist's...

  15. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...and putative pre-synaptic terminals with one vector. AAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby from Liqun...enhance axonal labeling and synaptophysin-tagged mRuby to enrich pre-synaptic labeling. Now available in...cytoplasmic targeted tdTomato and pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE...ready-to-use AAV5 viral vector. Synaptophysin-tagged GCaMP6s is enriched in the pre-synaptic terminal providing better...as a ready-to-use viral prep in serotype AAV1. Synapse targeting GCaMP6s from the Rylan Larsen lab are...

  16. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...DE3)   Basic IPTG-inducible strain containing T7 RNAP (DE3) F- ompT lon hsdSB (rB- mB-) gal dcm (DE3)...Tetracycline Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression F- ompT... ompT lon hsdSB (rB- mB-) gal dcm araB::T7RNAP-tetA General protein expression  BLR (DE3) Tetracycline...DE3)* Chloramphenicol (pLemo) Rhamnose-tunable T7 RNAP expression alleviates inclusion body formation. ...  T7 Express   IPTG-inducible expression of T7 RNAP from the genome; does not restrict methylated DNA...system. The DE3 lysogen expresses T7 RNA polymerase (RNAP) from the bacterial genome under control of the ..., which is inducible by the addition of IPTG. T7 RNAP is then available to transcribe the gene of interest...

  17. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...promoters for general RNAP II and RNAP III transcription; however, using viral LTRs as RNAP II promoters is ...to use an RNAP II promoter, whereas small RNAs (such as shRNA) are transcribed from the RNAP III promoters...is transcribed from DNA using an RNA polymerase (RNAP). In bacteria this is done by a single enzyme; however...of RNAs. To gain this specificity, the eukaryotic RNAP can recognize and bind to specific promoter elements...

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...A122D WPRE (AAV1) pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9...signal to noise ratio and is better localized for synaptic glutamate response. They generated variants optimized...activation kinetics and localization for imaging synaptic transmission. bioRxiv 2022.02.13.480251. doi: ...AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes...the most recent jGCaMP8 calcium sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1) pGP-AAV-syn-jGCaMP8f-WPRE...

  19. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...are released in each synaptic event and they are rapidly taken up by postsynaptic receptors and transporters...sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory cortex, while...ideal for video recordings of large populations of synapses and deep-brain fiber photometry.  Figure...promise to be a popular choice for a variety of synaptic imaging applications. Find iGluSnFR4 plasmids ...with tools like mass spectrometry, providing a snapshot of interacting proteins at a certain time and ...

  20. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    .../B), which cleaves the synaptic protein VAMP2, a protein essential for synaptic transmission. Cleavage... Hot Plasmids podcast Light-mediated block of synaptic transmission Article contributed by Klaus Wanisch...optogenetic tool that allows light-mediated silencing of synapses was developed by Matthew J. Kennedy’s and Chandra...demonstrated the utility of light-mediated suppression of synaptic transmission in brain slices from mouse, and showed...
Showing: 1 - 20 of 130 results