...recovery period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the.... As a result, a shorter recovery period of 30 minutes is possible with AmpR. So why even look at another...transformation recovery period than amp or carb (60 minutes). Pros: Widely available, cost-effective, confers...

...than 30 minutes and only requires incubating the columns with 1M phosphoric acid for 3 minutes followed...syringe-based method can be completed in 20 to 30 minutes. Additionally, while commercial plasmid prep kits...

...presented in a series of quick (10 minute) or very quick (2 minute) talks so we got to hear from everyone...Virginia Weis) Sea anemone Aiptasia - algae Breviolum minutum symbionts are important models for solutions to...

...microliter of RNA targets in a 40-minute incubation  Time: ~90 minutes Equipment needed: 37°C heat block...:10-100 copies of RNA per microliter Time: ~40 minutes Equipment required: 37°C heat block and 62°C heat...

...Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology of Biotin” J. Nutr, 129 (2009a): 477–...forgotten vitamin.” American Journal of Clinical Nutrition 75.2 (2002): 179–180. PubMed PMID: 11815306. 4...

...enhance the growth of mycoplasmas. The serum is nutrient-rich and also the best source for mycoplasma proliferation... longer time. When they come in contact with a nutrient source, they start proliferating shortly. Once...to the liquid or semi-solid medium (containing nutrients essential for mycoplasma growth). Infected cell...

... incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect...your timer to ding. If you’re shaving off a few minutes here and there, and seeing high background in your...

...showed a rise in transcription in as little as 30 minutes after light stimulation. Under those optogenetic...Second, induction of transcription occurs within minutes of light exposure. Third, the response can be graded...

...profile. To see how to create ReFigures in a few minutes, visit our YouTube Channel. Currently we can only...ReFigure by creating ReFigures and answering this 2 minute survey! You can also find ReFigure on Twitter (...

...obsession with the combination of chocolate and peanut butter), but it’s an undeniably difficult connection...sucrose…..sounds like the gateway to chocolate and peanut butter to me. What’s on the fiber horizon? The...

...frankenbody is that it remains bound to HA epitopes for minutes in living cells, meaning that scientists can track...nuclei in a developing zebrafish embryo over an 80 minute time course.  By stitching together a stable scaffold...

...during Zoom lab meetings to chip in ideas and take minutes that can be accessed by anyone at any time. This...simply moves away from the desk to switch off a few minutes. The host could also set aside Breakout Rooms to...

...“leaf” with similar permeability of water and nutrients and colonization opportunities for bacteria. To...the control of promoters responsive to water or nutrient levels can help him determine whether the artificial...

...the selected card on the board game. They had 35 minutes to develop a strategy and build a transfer plasmid... manipulate human stem cells. Then they had 10 minutes to expose their strategy, justifying each single...

... regular check-ins with their employees. 15-30 minutes weekly or monthly will usually suffice to keep ...scope” above) or if you can cut it down to thirty minutes. Keep it moving: If you tend to get stuck diving...

... meeting on the calendar even if it is just 15 minutes a week. Clearly identify constraints and boundaries...Update frequently with progress reports, meeting minutes and plan changes. Written documentation helps make...

...experiment (labeling tubes never takes longer than 5 minutes, right?) As a result, I’d always end up stressed... forever grateful, I started setting aside 3-5 minutes to sit quietly at my bench before the start of ...

...maintained when grown on media containing the missing nutrient. Scientists can exploit these host mutations by...auxotrophy. When grown on media NOT containing the nutrient, the host cells will die unless they have incorporated...

...Excuse me, I seem to have lost my place. I need a minute.” I then pulled out my notebook, opened it, found...presenting. The whole process took just under a minute. I can't say the journal club was a rousing success–the...

...activation, exhibits stabilized activity for over 30 minutes and can be deactivated using yellow light (590nm...-mcherry-C1-iC++_CA - A slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin...