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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 164 results for: Nut

Showing: 21 - 40 of 164 results

  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...microliter of RNA targets in a 40-minute incubation  Time: ~90 minutes Equipment needed: 37°C heat block...:10-100 copies of RNA per microliter Time: ~40 minutes Equipment required: 37°C heat block and 62°C heat...

  3. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology of Biotin” J. Nutr, 129 (2009a): 477–...forgotten vitamin.” American Journal of Clinical Nutrition 75.2 (2002): 179–180. PubMed PMID: 11815306. 4...

  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...enhance the growth of mycoplasmas. The serum is nutrient-rich and also the best source for mycoplasma proliferation... longer time. When they come in contact with a nutrient source, they start proliferating shortly. Once...to the liquid or semi-solid medium (containing nutrients essential for mycoplasma growth). Infected cell...

  5. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ... incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect...your timer to ding. If you’re shaving off a few minutes here and there, and seeing high background in your...

  6. Let There Be LITE Plasmids

    Type
    Blog Post
    ...showed a rise in transcription in as little as 30 minutes after light stimulation. Under those optogenetic...Second, induction of transcription occurs within minutes of light exposure. Third, the response can be graded...

  9. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...obsession with the combination of chocolate and peanut butter), but it’s an undeniably difficult connection...sucrose…..sounds like the gateway to chocolate and peanut butter to me. What’s on the fiber horizon? The...

  11. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...the selected card on the board game. They had 35 minutes to develop a strategy and build a transfer plasmid... manipulate human stem cells. Then they had 10 minutes to expose their strategy, justifying each single...

  12. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...during Zoom lab meetings to chip in ideas and take minutes that can be accessed by anyone at any time. This...simply moves away from the desk to switch off a few minutes. The host could also set aside Breakout Rooms to...

  13. How to Lead a Great Meeting

    Type
    Blog Post
    ... regular check-ins with their employees. 15-30 minutes weekly or monthly will usually suffice to keep ...scope” above) or if you can cut it down to thirty minutes. Keep it moving: If you tend to get stuck diving...

  14. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... meeting on the calendar even if it is just 15 minutes a week. Clearly identify constraints and boundaries...Update frequently with progress reports, meeting minutes and plan changes. Written documentation helps make...

  15. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...experiment (labeling tubes never takes longer than 5 minutes, right?) As a result, I’d always end up stressed... forever grateful, I started setting aside 3-5 minutes to sit quietly at my bench before the start of ...

  16. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...maintained when grown on media containing the missing nutrient. Scientists can exploit these host mutations by...auxotrophy. When grown on media NOT containing the nutrient, the host cells will die unless they have incorporated...

  18. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ... RNA-protein interaction.  In as little was one minute BASU generated a strong streptavidin signal (~250...tested at its optimum 18 hour time point, but one minute of labeling with BASU still generated a >30-fold...

  20. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...Excuse me, I seem to have lost my place. I need a minute.” I then pulled out my notebook, opened it, found...presenting. The whole process took just under a minute. I can't say the journal club was a rousing success–the...
Showing: 21 - 40 of 164 results