...if you’re anything other than a white male. “The Nuts and Bolts” is the title of the chapter that examines...

...Ravi SS (2017) Optimizing antibody expression: The nuts and bolts. Methods 116:51–62 . https://doi.org/10.1016...

...recommend no more than 40 minutes for the panel discussion leaving 20 minutes for audience questions (30...audience questions and an audience member asks a 5 minute question that is really a speech? You have all ...(possibly with an organizer) should set up a 30 minute call with each panelist to get to know their style... the panelists introduce themselves. I do a 1-2 minute introduction. If you chose good panelists, they...be good at talking, so they will easily take 5 minutes to introduce themselves and, voila, 1/2 of your...

...400 kDa MES for proteins <40 kDa 180 V, 30 minutes Good shelf life; runs at neutral pH (reduced ...400 kDa MOPS for proteins > 30 kDA 200 V, 30 minutes Good shelf life; runs at neutral pH (reduced ...your membrane. It requires anywhere from sixty minutes to overnight to run, and is recommended for very...proteins. A semi-dry transfer is faster — 3–30 minutes — and uses less buffer, but it is not as efficient... These proprietary systems are fast (often <10 minutes) and simple, utilizing individually packaged membrane... It requires long exposure times (up to thirty minutes!) to reach the limit of detection. Film also needs...images, reach their limit of detection quickly (~1 minute), and have a linear response over a broad dynamic...

...your incubation time on ice to 5 minutes (incubating the full 30 minutes will not significantly improve ...the PCR product and TOPO Vector.  3. Incubate 5 Minutes at Room Temperature: You can place your reaction...temperature, it is not recommended that you exceed the 5 minute time limit (lower transformation efficiencies have...); however, you may need to incubate for 20-30 minutes if your PCR product is at a low concentration or...

...an additional 5–10 minutes of incubation at 37 °C. Wash the membrane for 5–10 minutes 2–3x in fresh wash...solution and agitate for 30–45 minutes at 50 °C. Wash the membrane for 5–10 minutes 2–6x in fresh wash buffer...membrane in stripping solution and agitate for 5–30 minutes at room temperature. The incubation time will depend...

... roughly 30 minute chalk talk. You will not necessarily get to present the whole 30 minute version because...session lasts up to an hour. You will get 5-10 minutes to present your research vision uninterrupted and...begin working through your aims over the next 45 minutes. The faculty will ask you lots of questions. Your...members. As mentioned above, plan to give your 10 minute overview and address the aims in more depth in ...

...spend 25 minutes focusing on a task, like writing, and then take a 5-minute break. Each 30-minute interval...After four Pomodoros, you can take a longer 15-30 minute break. The method is named for the tomato or “Pomodoro...

...dye and exposed to light stained dead within 30 minutes of exposure. 90 seconds of illumination were sufficient... fish with the dye. Embryos exposed to just 12 minutes of illumination had fewer mCer3-labeled cells in...that KillerRed required longer exposure times (5 minutes vs 10 seconds) to achieve the same level of killing...reaching greater than 75% photobleaching after just 1 minute of exposure. MG-2I-dL5** experienced minor bleaching...

...out their own novel experiments in semester two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates...capabilities and laboratory and critical thinking skills (Knutson et al., 2010a,b). Classroom-based research projects...planarians. Developmental Biology. 457:119-127. Knutson, K., Smith, J., Wallert, M., Provost, J. 2010a....and Molecular Biology Education. 38(5):317-323. Knutson, K., Smith, J., Nichols, P., Wallert, M., Provost...

..., easy goal. For example, set a timer for five minutes and come up with as many solutions as you can in... is simply to throw out ideas.  When your five minutes are up, if you haven’t come up with any ideas obviously... or even very frequent, but establishing a few minutes every month dedicated to thinking about the future...time crunch, your break can be as simple as a few minutes of meditation or a walk to the break room for a...

...tracked at 5 minute intervals via Western blot. Phosphorylation of MEK1/2 peaks at just 5 minutes post-Raf1.... They did this by exposing psRaf1 to a short 2 minute pulse of 500 nm light (turn on psRaf1) followed...

...outline: Instead of getting bogged down in the minutia or coming off as a broken record, remember the ...sometimes, attendees can get sucked in to thirty-minute-long presentations, which constrains the number...pressed for time, limit yourself to five to ten minutes per poster. Ask the presenter to give the “elevator...

...recovery period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the.... As a result, a shorter recovery period of 30 minutes is possible with AmpR. So why even look at another...transformation recovery period than amp or carb (60 minutes). Pros: Widely available, cost-effective, confers...

...than 30 minutes and only requires incubating the columns with 1M phosphoric acid for 3 minutes followed...syringe-based method can be completed in 20 to 30 minutes. Additionally, while commercial plasmid prep kits...

...presented in a series of quick (10 minute) or very quick (2 minute) talks so we got to hear from everyone...Virginia Weis) Sea anemone Aiptasia - algae Breviolum minutum symbionts are important models for solutions to...

...Proteins; Symposium: Nutrition, Biochemistry and Molecular Biology of Biotin” J. Nutr, 129 (2009a): 477–...forgotten vitamin.” American Journal of Clinical Nutrition 75.2 (2002): 179–180. PubMed PMID: 11815306. 4...

...microliter of RNA targets in a 40-minute incubation  Time: ~90 minutes Equipment needed: 37°C heat block...:10-100 copies of RNA per microliter Time: ~40 minutes Equipment required: 37°C heat block and 62°C heat...

...enhance the growth of mycoplasmas. The serum is nutrient-rich and also the best source for mycoplasma proliferation... longer time. When they come in contact with a nutrient source, they start proliferating shortly. Once...to the liquid or semi-solid medium (containing nutrients essential for mycoplasma growth). Infected cell...

... incubation (10–20 minutes) at 70 °C, or a truly lengthy incubation (30–60 minutes) at 37 °C, if you suspect...your timer to ding. If you’re shaving off a few minutes here and there, and seeing high background in your...