...Cibelli George Daley Jin-Tang Dong Steven Dowdy Scott Friedman Konrad Hochedlinger Rudolf Jaenisch Keisuke...

...content for this page was generated with the help of Scott Lindner . Cut Fully functional CRISPR/Cas enzymes...

...detect proteins within a 96- or 384-well, flat-bottomed plate. The basic principle of an ELISA is that...or by binding to an antibody that is coating the bottom of the well), and then its concentration is detected...technique works by first coating the wells of a flat bottomed plate with a capture antibody that is specific...amount of an antibody that recognizes it, while the bottom of the wells are coated with another protein — ...light absorbed by a solution - of these wells is plotted against the known protein concentration. Importantly... Figure 5: A standard curve is generated by plotting OD against known concentrations of your target...protein. The OD of experimental samples can then be plotted along this line to calculate the protein concentration...

...control can be accurately quantified In western blotting, the relationship between protein abundance and...detection range of the assay is between the two dotted lines. Created with BioRender.com. To see why...“the most common error associated with Western blotting quantification” (Gilda & Gomes, 2013). To address...covered in our previous blog posts (1, 2) on western blotting. If the abundance of your POI and loading control...measurement will take place either before or after blotting for your POI. Figure 6: Total protein measurements...single-protein controls in semi-quantitative immunoblotting. Journal of Neuroscience Methods, 172(2), 250...Addgene Blog Antibodies 101: The Basics of Western Blotting Technical Design of a Western Blot Troubleshooting...

...Tissue Atlas (Link opens in a new window) . Western Blotting (WB) Proteins are extracted from cells, separated...

...engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) Toolkit includes...

...transduced with lentiCas9-blast. Anti-Cas9 Western blotting was performed on whole-cell extracts from stable...multiplicity of infection (MOI). Anti-vinculin Western blotting is a loading control. Pro-Tips: 1. It’s all...under blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts from the ... Checking Cas9 expression Overview: By Western blotting (Figure 2, pool), I knew the cells expressed Cas9...good image. You could probably also do Western blotting against GFP expression, but I didn’t. 2. In the... I could even also see the 85 bp product at the bottom of the gel in the BRAF gRNA-transduced samples ...

...gRNA Shuttle Mt U6.6 inFusion none S. pyogenes Parrott 50591 pCBC-DT2T3 see paper BsaI none S. pyogenes...

... 2, 5, 8, 9, rg*, PHP.eB, PHP.S Boyden 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a NLS-mCherry...

... each serial dilution is plotted on the x-axis and its absorbance is plotted on the y-axis. With the help...spectrophotometer and concentration versus absorbance is plotted for the known concentrations (standard curve), ...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline...this kind of software, don’t give up! Rather than plotting the entire data set, plot only the linear portion...

...or siRNA expression from the other. The Q system Potter et al. from Liqun Luo’s lab developed the Q system...functional in C. elegans. Subsequent work by the Luo and Potter labs has refined the Q system and introduced the...Neurosci 9(5) (2006): 703-9. PubMed PMID: 16582903. Potter, Christopher J., et al. “The Q system: a repressible...publication at Addgene. Wei, Xing, Christopher J. Potter, Liqin Luo & Kang Shen. “Controlling gene expression...from this publication at Addgene. Riabinina O, Potter CJ. The Q-System: A Versatile Expression System...

...following two articles: Kunzelmann, et al. G3 2016 Bottcher, et al. Nucleic Acids Res. 2014 The Förstemann...

...specifically how dry ice can affect virus from Addgene. Bottom line: there’s good news and there’s bad news. The...by a short 2-hour exposure to pH as low as 2.5 (Potter et al., 2014). They also showed that even 24 hours...infectivity after 3 weeks of exposure to pH 5.1 (Potter et al., 2014). Based on our results and the results...2013 Apr;10(4):278-9. PubMed PMID: 23538862. 2. Potter M, Lins B, Mietzsch M, Heilbronn R, Van Vliet K...

...Antiviral Strategy to Combat SARS-CoV-2 and Influenza. Abbott, et al. Cell. 2020 May 14;181(4):865-876.e12. (...

...-Linked Immunosorbent Assay) Southern/Northern blotting Cell surface labelling Attaching polypeptides ...including but not limited to in situ hybridization, blotting (Northern and Southern) and affinity binding. ...PubMed PMID: 10470036. 3. Said, H. M. “Biotin: The forgotten vitamin.” American Journal of Clinical Nutrition...non-radioactive method for small RNA detection by northern blotting.” Rice 7.1 (2014): 1–7. PubMed PMID: 26224555....

...top strand first followed by the bottom strand versus nicking the bottom strand first followed by the top...PCR-generation of a mutant version of the top and bottom strands of the backbone plasmid. Unlike SDM, which... Does it matter what order I nick the top and bottom strands of the plasmid? The authors’ compared nicking...

...standard confocal microscopy. Image adapted from (Bottanelli et al., 2016) under CC-BY license. Combining...experiments! References and resources References Bottanelli, F., Kromann, E. B., Allgeyer, E. S., Erdmann...Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins, A., Klaubert...-39655-3 Wilhelm, J., Kühn, S., Tarnawski, M., Gotthard, G., Tünnermann, J., Tänzer, T., Karpenko, J.,...

...pAAV-CAG-eYFP CAG EYFP Control Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-...

...version of the AAV decision tree can be found at the bottom of this page. For more in depth descriptions of...

...      *If you submitted the names Abi, Lin, or Blottie (our top 3 choices), we'll be in touch with you... three choices.   Fig. 1: Lin, Abi, or Blottie? Which one suits me best?     But between these... options, we simply can't decide! Abi, Lin, or Blottie? Which name best fits our new immunoglobulin friend...