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We narrowed to 221 results for: Rras

Showing: 21 - 40 of 221 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... develop an array using 3 distinct spacer-repeats, you could easily create 7 unique arrays (e.g. for spacers... system allows you to assemble type II-A CRISPR arrays for dCas9-based transcriptional repression. The... spacers A, B, and C, you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab E. coli CRMAGE...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” Nature 516(7531...Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression...

  2. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...useful anywhere I work in the future; they are transferrable across most, if not all, industries and work...add an extra shine to your work. Knowing your transferrable skills allows you to build on them even more... an article identifying gaps in scientists' transferrable skills. In describing the limitations of the...there is also value in knowing and owning your transferrable skills yourself so that you can grow them even...mind working, take a look at the short list of transferrable skills here and consider which of these skills...

  3. CRISPR Guide

    Type
    Collection
    ...expressing Cas9 and a gRNA will result in a diverse array of mutations (see: Validate genetic modification... a given genome. Some more recent libraries use arrays of multiple gRNAs to target paralogs or lncRNAs...R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome ...Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G., & Zhang, F. (2013). DNA targeting...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature , 516 (... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors...efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI...Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter vector...cloning DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast, or FLASH ...

  5. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...Estamos orgullosos de anunciar que CRISPR 101: Una herramienta de edición ahora es parte oficial de nuestra ...libros electrónicos. Descargar CRISPR 101: Una herramienta de edición Addgene tiene una gran audiencia internacional...are pleased to announce that CRISPR 101: Una herramienta de edición is now an official part of our eBook...eBook collection.  Download CRISPR 101: Una herramienta de edición  Addgene has a large international audience...

  6. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...programmed cell death" plays a pivotal role in an array of biological processes including development, the...Upon cleavage of the sequence, β10 and β11 can rearrange to their normal antiparallel position and fluoresce...applied to other fluorescent proteins to create an array of multicolor reporters that will enable imaging...dying at the wrong time and/or place can lead to an array of diseases and pathologies such as neurodegenerative...

  7. Zinc Finger Consortium Reagents

    Type
    Collection
    ...finger arrays via OPEN method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular... Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc...

  8. TALEN Expression Vectors

    Type
    Collection
    ...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH methods...which DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH methods...three of these methods yield TAL effector repeat arrays with identical DNA and amino acid sequences (i....

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying the...working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition of the viral...viral vector with bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain...

  10. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Institute Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets...DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is ... can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with...

  11. TALEN Engineering

    Type
    Collection
    ...three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme...of these platforms assemble TAL effector repeat arrays on a single architectural framework and the DNAs... are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs which...

  12. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... slides or notes. Listen to their stories and narratives (see below “Tell a story”) and note how they ...the point.   What makes a story? A story is a narrative connecting cause and effect. Stories can and should...obstacles. Take time to consider the order of your narrative. Scientists do this all the time, you just need...Industry Job Talk What’s Your Story? By Herminia Ibarra and Kent Lineback Resources on the Addgene Blog...

  13. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... to replicate at a higher frequency (Summers & Sherratt, 1984; Summers et al., 1993). These larger plasmids...the monomeric version of the plasmid (Summers & Sherratt, 1984; Summers et al., 1993; Summers, 1998). Multimeric...monomers and various forms of multimers (Summers & Sharratt, 1984; Summers et al., 1993). In these cases, ...2958.1993.tb01648.x. PMID: 8361350. Summers DK, Sherratt DJ. Multimerization of high copy number plasmids...

  14. Finding Your Science Policy Path

    Type
    Blog Post
    ...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans...SCI. Many thanks to our guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at...at the Veterans Health Administration. Steph Guerra, PhD, is currently a AAAS Science and Technology Policy...equitable for all. You can follow her on Twitter at @Steph_Guerra Additional resource on the Addgene blog: Browse...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular... 2017 Nov 10;8(1):1412. Justin Taraska , David Perrais pH Sensor for intracellular chloride and pH (ClopHensor...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...

  16. CRISPR References and Information

    Type
    Collection
    ...pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF, 129 KB Marraffini Bacteria: pCRISPR...pSL1180polyUBdsRED PDF, 597 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9...

  17. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...http://en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712...CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan; 339 (6121): 819...

  18. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...to explore this role, how to seek out such an arrangement, and how to operate once in that position. What... plan as well. Making logistical and funding arrangements Visiting scientist positions take commitment...structured visiting program, you will have to make arrangements on your own. This will include, but is not limited...

  19. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...produce functional R-mAbs. It was recognized that an aberrant IgG light chain transcript was expressed by some...the restriction enzyme BciVI was present in the aberrant light chain sequence, but was predicted to occur..., Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. A toolbox of IgG ...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ... test many variables all at once. CRISPR CRISPR arrays and libraries depend on many gRNAs delivered with... MoClo kit, is a prime choice for assembling an array of targeting constructs and delivering them to these...start from scratch! As you can imagine, there is an array of distinct goals within metabolic engineering, ...
Showing: 21 - 40 of 221 results