...Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For...Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding protein,...Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding protein ...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2-OR1...Arabinose, Anhydrotetracycline, lactose, IPTG Richard Murray Plasmids based off of pBESTluc from Promega. Contain...

... develop an array using 3 distinct spacer-repeats, you could easily create 7 unique arrays (e.g. for spacers... system allows you to assemble type II-A CRISPR arrays for dCas9-based transcriptional repression. The... spacers A, B, and C, you could obtain arrays A, B, C, AB, AC, BC, and ABC). Nielsen Lab E. coli CRMAGE...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system.” Nature 516(7531...Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9-Mediated Multiplex Transcriptional Repression...

...estrogen receptor 2 (ER beta) ESRRA Plasmids ESRRA, ERR1, ERRa, ERRalpha, ESRL1, NR3B1 estrogen-related...

...useful anywhere I work in the future; they are transferrable across most, if not all, industries and work...add an extra shine to your work. Knowing your transferrable skills allows you to build on them even more... an article identifying gaps in scientists' transferrable skills. In describing the limitations of the...there is also value in knowing and owning your transferrable skills yourself so that you can grow them even...mind working, take a look at the short list of transferrable skills here and consider which of these skills...

...to harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic functions...that can be used to engineer viruses for a wide array of research purposes. Ready-to-use viral preparations...that can be used to engineer viruses for a wide array of research purposes. Which Type of Virus Should...

...Estamos orgullosos de anunciar que CRISPR 101: Una herramienta de edición ahora es parte oficial de nuestra ...libros electrónicos. Descargar CRISPR 101: Una herramienta de edición Addgene tiene una gran audiencia internacional...are pleased to announce that CRISPR 101: Una herramienta de edición is now an official part of our eBook...eBook collection.  Download CRISPR 101: Una herramienta de edición  Addgene has a large international audience...

...Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors...efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI...Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter vector...cloning DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast, or FLASH ...

...expressing Cas9 and a gRNA will result in a diverse array of mutations (see: Validate genetic modification... a given genome. Some more recent libraries use arrays of multiple gRNAs to target paralogs or lncRNAs...R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome ...Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G., & Zhang, F. (2013). DNA targeting...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature , 516 (... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

...programmed cell death" plays a pivotal role in an array of biological processes including development, the...Upon cleavage of the sequence, β10 and β11 can rearrange to their normal antiparallel position and fluoresce...applied to other fluorescent proteins to create an array of multicolor reporters that will enable imaging...dying at the wrong time and/or place can lead to an array of diseases and pathologies such as neurodegenerative...

...may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying the...working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition of the viral...viral vector with bacteria designed to minimize rearrangements such as NEB Stable or use a standard strain...

...finger arrays via OPEN method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular... Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc...

...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH methods...which DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH methods...three of these methods yield TAL effector repeat arrays with identical DNA and amino acid sequences (i....

...Institute Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets...DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is ... can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with...

...three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme...of these platforms assemble TAL effector repeat arrays on a single architectural framework and the DNAs... are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs which...

... slides or notes. Listen to their stories and narratives (see below “Tell a story”) and note how they ...the point.   What makes a story? A story is a narrative connecting cause and effect. Stories can and should...obstacles. Take time to consider the order of your narrative. Scientists do this all the time, you just need...Industry Job Talk What’s Your Story? By Herminia Ibarra and Kent Lineback Resources on the Addgene Blog...

... to replicate at a higher frequency (Summers & Sherratt, 1984; Summers et al., 1993). These larger plasmids...the monomeric version of the plasmid (Summers & Sherratt, 1984; Summers et al., 1993; Summers, 1998). Multimeric...monomers and various forms of multimers (Summers & Sharratt, 1984; Summers et al., 1993). In these cases, ...2958.1993.tb01648.x. PMID: 8361350. Summers DK, Sherratt DJ. Multimerization of high copy number plasmids...

...This post was contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans...SCI. Many thanks to our guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at...at the Veterans Health Administration. Steph Guerra, PhD, is currently a AAAS Science and Technology Policy...equitable for all. You can follow her on Twitter at @Steph_Guerra Additional resource on the Addgene blog: Browse...

...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular... 2017 Nov 10;8(1):1412. Justin Taraska , David Perrais pH Sensor for intracellular chloride and pH (ClopHensor...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...

...PDF 165.1 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF 125.6 KB Marraffini Bacteria: pCRISPR...pSL1180polyUBdsRED PDF 583.3 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9...

...http://en.wikipedia.org/wiki/File:Crispr.png Barrangou R, Horvath P. Science. 315, 2007 Jan; 1709–1712...CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier E, Horvath P, Moineu..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan; 339 (6121): 819...