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We narrowed to 91 results for: SHR;

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Showing: 21 - 40 of 91 results

  1. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...deleted. shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs... excise the shRNA to return gene expression to physiological levels. In lox-STOP-lox shRNA constructs,...constructs, Cre expression promotes shRNA expression. Gene Switch: These constructs contain two genes of interest...

  2. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris) luciferase and engineered...cruciata) Natural 562 64 Luciferin ATP No Luminous shrimp(Oplophorus gracilirostris) Natural 455 19 Coelenterazine...

  4. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ... for: a wide range of DNA edits.   Cas3 – The shredder What sets Cas3 apart from Cas9 and almost all .... This system could be used in lieu of siRNA or shRNA for efficient, multiplexable RNA knockdown. Cas13...

  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...they tested truncated ADARDDs to see if they could shrink the construct. They identified ADAR2DD(delta984...Although REPAIRv1 knockdown is more precise than shRNA knockdown, it still displays substantial off-target...

  7. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... size distribution. When comparing LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown...a distinct contrast to the hundreds of observed shRNA off-targets. They subsequently used catalytically...

  8. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia et al., 2016). Despite the...10.1038/nbt.2969 Waltz E (2016) Gene-edited CRISPR mushroom escapes US regulation. Nature 532:293–293 . https...

  9. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ... Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ...Alghamdi, R. A., Tomancak, P., Reynaud, E. G., & Shroff, H. (2017). Assessing phototoxicity in live fluorescence...

  10. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse of bioluminescence, NLuc... Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ...

  12. Retrovirus Plasmids

    Type
    Collection
    ...Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs Packaging plasmid — ...Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression with GFP expression. See plasmid 8452...27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA. Expresses rtTA. See Zuber et al. (2011) for additional...

  13. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...tdTomato from the EF1 promoter pLL3.7-TNFa-shRNA3 72598 Expresses shRNA against mouse TNF-alpha mRNA from the...

  19. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...insert. pLKO.1 – scramble shRNA Negative control vector containing scrambled shRNA. psPAX2 Packaging plasmid...Map of pLKO.1 A.3 Related plasmids B. Designing shRNA Oligos for pLKO.1 B.1 Determine the optimal 21-mer... Order oligos compatible with pLKO.1 C. Cloning shRNA oligos into pLKO.1 C.1 Recommended materials C.2...which The RNAi Consortium has built a library of shRNAs directed against 15,000 human and 15,000 mouse ...genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific community...lentiviral vector chosen by the TRC for expression of shRNAs. pLKO.1 can be introduced into cells via direct...selection. Figure 1 : Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has...
Showing: 21 - 40 of 91 results