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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 568 results for: Star

Showing: 21 - 40 of 568 results

  1. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...all seems lost in the War of Light, Look to the stars –                                     -for HOPE ... in the mall. I took action, joined a gym, and started running on a treadmill. After being with the company...treatments, if available. As part of the team, I started adding mile after mile on my poor, poor legs. ...

  9. AAV for Neuronal Tracing

    Type
    Collection
    ...is provided to the starter cell in trans , via delivery by an AAV. Since this starter cell now has all ...TVA and G-protein is delivered to the starter cell. (2) The starter cell now expresses TVA and G-protein... RAVB is delivered to the starter cell, and specifically targets the starter cell via the EnvA receptor...). Thus, G-deleted rabies can infect a single "starter" cell, but new virus cannot spread to any neighboring...can spread to cells that are projecting to the starter cell). Once in the secondarily infected cells that... the targeting of G-deleted rabies to specific starter cells, the tropism of this virus was modified so...receptor. RABV is able to replicate in the starter cell using the G-protein, which is supplied in trans . (...

  11. Plan Your Experiment

    Type
    Collection
    ...This page provides a general framework to get you started using CRISPR in your research and setting up CRISPR...understanding of your experimental goal, you are ready to start designing the specific components (Cas enzyme and...aware that there could be downstream translation start sites that could allow for expression of a truncated...elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to be fused to transcriptional...to be within a given range of the transcription start site in order to function effectively. Select gRNAs...

  13. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...pLysE (CamR) Expression of toxic proteins BL21 star (DE3)   Lacks functional RNaseE which results in... you wait too long to induce, your culture will start collecting dead cells, and, conversely, you cannot...

  14. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...Alternate Prefix: GAATTC GCGGCCGC T TCTAGA TG (contains start) Prefix Enzymes: EcoR1 , NotI , XbaI Suffix: T ACTAGT...Features: Scar encodes Gly-Ser in-frame with prefix start codon; Employs high-efficiency enzymes. Prohibited... Pstl Scar: ACCGGC Features: Modified RFC 10 w/ start and stop codons; Creates in-frame fusions w/ linker...

  15. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Michael J Fox Foundation MJFF 40386 pBACgus_Sumostar/LRRK2 1867-2186 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40387 pBACgus_Sumostar/LRRK2 1867-2186_G2019S LRRK2 His, TEV polH...Parkinson's Michael J Fox Foundation MJFF 40388 pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S LRRK2 His, TEV...Parkinson's Michael J Fox Foundation MJFF 40389 pBACgus_Sumostar/LRRK2 1867-2186_T2031E LRRK2 His, TEV polH...Parkinson's Michael J Fox Foundation MJFF 40390 pBACgus_Sumostar/LRRK2 1867-2141 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40391 pBACgus_Sumostar/LRRK2 1867-2161 LRRK2 His, TEV polH Parkinson's...Parkinson's Michael J Fox Foundation MJFF 40392 pBACgus_Sumostar/LRRK2 1867-2176 LRRK2 His, TEV polH Parkinson's...

  16. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...expectations to the best of our knowledge from the start. However, we know there’s a chance that things may...general estimated turnaround time above. After starting viral production, if we determine that we will...

  17. AAV Packaged on Request

    Type
    Collection
    ...By using AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep...depend on your location. Share Your Results Once you start getting results from your AAV experiments, remember...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...protein production. Other Sensors and Tools Optimized STARR-seq (Self-Transcribing Active Regulatory Region)...reporter. These plasmids improve on the original STARR-seq by using the bacterial ORI as the core promoter...

  19. CRISPR Guide

    Type
    Collection
    ...various applications of CRISPR, and help you get started using CRISPR in your own research. CRISPR Basics...Overview of CRISPRi/a Targeting dCas9 to transcription start sites is sufficient to repress transcription by ...recruits Cas3. Cas3 nicks the non-targeting strand and starts degrading the single-stranded DNA, unraveling DNA...
Showing: 21 - 40 of 568 results