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Showing: 21 - 40 of 165 results

  1. Adenovirus Plasmids

    Type
    Collection
    ... 5 (HAdV5) genome that can be assembled in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...our guest blogger Kutubuddin Molla from the Pennsylvania State University Kutubuddin Molla is an Ag-biotechnologist...This post was contributed by Kutubuddin Molla, a Fulbright Visiting Scholar at the Pennsylvania State... Institute, Cuttack, Odisha, India. Currently Kutubuddin is a Fulbright Visiting Scholar at the Pennsylvania...

  3. mTOR Pathway

    Type
    Collection
    ...Ribosomal protein S6 kinase B1 TSC1/2 TSC1 TSC2 Tuberous sclerosis VHL Von Hippel-Lindau tumor suppressor...

  4. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...way! Many thanks to guest blogger Kaustubh Kishor Jadhav! Kaustubh Kishor Jadhav, MSc is a Research Assistant...This post was contributed by guest blogger, Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute...lowered pH due to microbial contamination. Photo by Kaustubh Kishor Jadhav. Mycoplasmas can remain in the...

  5. p53 Pathway

    Type
    Collection
    ...3 Siah Siah E3 ubiquitin protein ligase 1 TSC2 Tuberous sclerosis 2 TSP1 Thrombospondin 1 Return to top...

  6. Viral Production

    Type
    Collection
    ...matching system that follows the sample from the DNA tube all the way to the cleared viral preparation pool...

  7. Ras Pathway

    Type
    Collection
    ...metastasis TP53 Tumor protein p53 TSC TSC1 TSC2 Tuberous sclerosis VAV1 Vav guanine nucleotide exchange...

  8. Clear and Accessible Writing

    Type
    Blog Post
    ...used to convey the difference between a gradient tube before and after ultracentrifugation so that a scientist...each layer. The right panel illustrates a gradient tube before and after ultracentrifugation. The viral ...the bevel pointed up. A syringe at the top of the tube allows air to enter the column so the virus will...

  9. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...method procedure The procedure (illustrated in the YouTube video below) is simple: After obtaining antibiotic-resistant...are mixed with 3 ml of 2X DMEM in a 50 ml sterile tube (for 60 mm dishes only 1.5 ml of each are used)....agarose plug by rapid pipetting, as shown in the YouTube video. Before returning the plate to the incubator...

  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... K, et al (2015) Resolving bundled microtubules using anti-tubulin nanobodies. Nat Commun 6:7933. https... start re-conceptualizing actin filaments or microtubules. This issue is shared by other SRM techniques...

  11. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...videos on Addgene’s YouTube channel.   Figure 4: Global viewership of the Addgene YouTube channel, 2023. ...to protocols. In 2013, we launched the Addgene YouTube channel to add a strong visual component to our...

  12. Hot Plasmids - August 2020

    Type
    Blog Post
    ...growing tips of microtubuli and plasmids tagged with MapTau to label entire microtubules. Find these fluorescent...best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found that too...

  13. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...same tube. You can clone up to 4 DNA fragments, in a specific order and orientation, in one tube, into...possible to set up the BP and LR reactions in the same tube, speeding up the cloning of the attB-PCR products...

  15. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    ... — check out Addgene’s new Quick Tip series on YouTube! In this series, we share quick tips to help you...video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips, protocols, animations, ...

  16. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation...video content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog...

  17. Deck the Lab 2022!

    Type
    Blog Post
    ...right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and seal up those plates...decorated their labs with everything from Falcon tube trees to giant paper mache Krampus performing routine...

  18. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...to sit down and talk with us. Tom McFadden is a YouTuber and science educator looking for creative ways...communicate science. Learn more about Tom on his youtube channel and at sciencewithtom.com.   Special thanks...

  19. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ... safely recycle these materials either.  Test tubes aren’t cheap everywhere Lab supplies and equipment...Coast where her first job was to wash Eppendorf tubes which were to be used for 3 months before they could...
Showing: 21 - 40 of 165 results