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We narrowed to 152 results for: Tore

Showing: 21 - 40 of 152 results

  1. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...high/low) and patterns (tissue/timing) can be monitored under various conditions without necessarily modifying...insert your construct to generate a collection of monitored genes and mutants - viral integration, transposon...

  3. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...

  4. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...great deal more information that one might like to store about FPs than is currently represented at fpvis...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ... abundance of each barcoded clone can then be monitored over time by sequencing the barcodes in the population...receptor (EGFR) with the ClonTracer library and monitored population dynamics after treatment with EGFR ...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately for amplification.... Purified high-titer rAdV is then dialyzed and stored at -80C. Purification and concentration kits that...

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...in a couple of days, some researchers prefer to store freshly harvested virus at 4 °C instead of storing...highly sensitive to freeze-thaw cycles and should be stored in single-use aliquots. Never use a polybrene solution...

  10. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months before... completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer...

  11. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...aliquot them into a number of vials, freeze them, and store all but one in liquid nitrogen. The frozen vials...master stock. This ensures that you have many cells stored in their original condition and can replace your...

  14. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

  16. Important Considerations When Using AAVs

    Type
    Blog Post
    ...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment... liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be...

  18. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...photoswitchable proteins derived from the fungal photoreceptor, Vivid (VVD, N. crassa) (Kawano et al., 2015...single-chain fusion proteins by fusing Cas9 to photoreceptors. One prime example was from the lab of Michael...

  19. Antibodies

    Type
    Collection
    ...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...
Showing: 21 - 40 of 152 results