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We narrowed to 107 results for: Tore

Showing: 1 - 20 of 107 results

  1. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...and it will impact the time you will be able to store your sample without having substantial degradation...things when you elute your DNA: where you plan to store it and for how long. The most common solution is...plasmid DNA can help you choose the best way to store and elute the DNA. References Murakami M (2013...

  2. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ...School and Paratore is the General Auditor at Tufts Health Plan. The addition of Katz and Paratore onto the...Interactive. Learn more about Carl Paratore, MBA, CPA Mr. Paratore serves as the General Auditor at Tufts...re excited to welcome Karen Katz, Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors. Katz...that Addgene was looking for new board members. Paratore: Kristen Darby, who’s also a board member, reached...necessary information that’s required to do research. Paratore: What I’ve been most impressed with is the level...potentially also be very helpful to the scientific community.Paratore: I’ve certainly had a front row seat watching...be, an interesting legal and professional challenge.Paratore: The promise there is the ability share the...

  4. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...pH. We compared the dry ice-stored samples to controls that were only stored in a -80°C freezer and not...of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results showed that...thawed, there was no effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once...reduction (from 7.4 to 5.0) in the pH of samples stored in our AAV tubes (Fig. 1). It’s important to note...the pH effects of dry ice storage. To do this, we stored samples on dry ice for 72 hours and then vented...compared these samples to controls that were only stored at -80°C. We then thawed the tubes after the indicated...had the same pH as control tubes that were never stored on dry ice/exposed to CO2 (Fig. 5). Basically, ...

  5. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...the tool, and develop new applications for magnetoresponsive proteins. Motivated by curiosity Although...Maria dreamed about the possibilities of a magnetoresponsive fluorescent protein. Controlling an optogenetic...self-labeling FlavinTag made a reliable one-component magnetoresponsive system that could be expressed and purified...thrilled to see that MagLOV’s “astonishing” magnetoresponse was clearly visible whether expressed in E....respond to magnetic fields?” and “What are magnetoresponsive fluorescent proteins good for?”, and while... Photochemical and structural insight into magnetoreception. Protein Science, 30(8), 1521–1534. https:...of magnetic field effects relevant to avian magnetoreception. Nature Chemistry, 8(4), 384–391. https://...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...the lab. These barcodes additionally allow us to store multiple types of tube for each plasmid we have ...transformation After the DNA has been received and stored, it is put into E. coli strains that have been ...that is received is immediately transformed and stored. Some samples need to be transformed with a micropulser...samples (98.2%) transform the first time and can be stored right away. Every week, the lab performs an average...Barcoded tubes come in many forms. The 2D barcode can store a lot of information in a small space and is ideal...there multiple freezers that the samples can be stored in? Different shelves? Different racks on each ...

  7. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...what temperature to store your antibodies at, but it also matters what they’re stored in - their buffer....antibody, the second thing you’ll want to do is store it away safely. The first thing you should do is...primarily used in Western blots and ELISAs, cannot be stored in sodium azide at all, as it inhibits HRP. In ...sensitive, so antibodies conjugated to them need to be stored either in a dark, opaque vial or wrapped in aluminum...

  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...vivo studies. Preparations are then aliquoted and stored at -80 °C. Titer Titering is either performed by...The preparations are then aliquoted, frozen, and stored at -80 °C. Concentrated lentiviral preparations...then resuspended in PBS, aliquoted, frozen, and stored at -80 °C. Titer All titering is performed on lentiviral... lentiviral preparations that have been stored at -80 °C and thawed. This ensures that any loss of titer...production. Barcoding allows our DNA and virus to be monitored throughout production, ensuring the identity of...

  9. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...spreadsheets, Word docs, and the like is likely stored in online file storage tools like Dropbox, Box,...like image banks and sequencing files is likely stored in institutional and lab servers specifically designed... conveniently reference specific pieces of data stored externally in the record of research compiled in...tools, as illustrated in this video, and to data stored on institutional and lab servers. Connectivity ...

  10. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...which can be stored in liquid nitrogen long term, preferably some of these vials will be stored in a separate...separate backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial can then... recorded on the outside of the vial and in a cryostore log. Keeping good passage records allows you to...

  11. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...like rotting eggs but has a longer shelf life when stored properly. Because BME is not as strong as DTT, ..., while expensive, reduce protocol time, can be stored at 4 °C, and are very consistent gel-to-gel.  Ladder...inexpensive and easily made in the lab. They can be stored at your bench during the procedure and at 4 °C ...Fluorescent proteins are stable, so blots can be stored for weeks to months without loss of signal. While...

  13. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...initial reversal drive cannot restore the exact original sequence, it can restore the original protein-coding...using a recoding strategy; a subsequent drive can restore the wild-type sequence (save for the residual sgRNAs...

  14. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... It is an easily accessible organ that can be monitored non-invasively over time. Importantly, it is an...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...proteins (NIR FPs) were derived from natural photoreceptors incorporating biliverdin IXα (BV) autocatalytically... derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular brightness than...were derived from cyanobacteriochrome (CBCR) photoreceptors (Oliinyk et al., 2019). They were engineered...

  16. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...'Regan Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a ...full of information about how to deposit plasmids, store your plasmids or viral items, technical support,...

  17. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... Best Way to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris...

  18. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    Published
    Jan. 28, 2014, 3:10 p.m.
    ...buffers. The competent cells are then aliquoted and stored at -80oC until we are ready to perform transformations...competent cells are good for at least a year when stored at -80oC.   For each new batch of competent cells...

  19. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...high/low) and patterns (tissue/timing) can be monitored under various conditions without necessarily modifying...insert your construct to generate a collection of monitored genes and mutants - viral integration, transposon...
Showing: 1 - 20 of 107 results