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...mechanism unknown)   Chen et al., 2023 AAV9.452sub.LUNG1 Lung: ATII cells Mice N/A Goertsen et...type II cell transduction in the rodent lung AAV9.452sub.LUNG1 is an engineered capsid derived from AAV9...has tropism for lung cells, and has increased overall transduction throughout the lungs. Like other engineered...AAV9 that effectively transduces mouse lungs, targeting alveolar epithelial type II cells (Goertsen, Goeden... does express in the brain. Both AAV9 and AAV9.452sub.LUNG1 have significantly higher off-target expression...including PAL1D, are detargeted from the liver, kidney, lung, and thymus, and transduce the heart and spleen ...Flytzanis, N. C., & Gradinaru, V. (2022). Targeting the lung epithelium after intravenous delivery by directed...

...Cas9s with novel PAM recognition In 2015, Keith Joung’s lab performed a series of positive selection screens...Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev...Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing with CRISPR-Cas13....Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered CRISPR-Cas9 nucleases with ...Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK (2016) Genome-wide specificities of CRISPR-Cas...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (...

..., 20234Kaludov et al., 2001; Walters et al., 20015Dhungel et al., 2025 Depending on how you count, there...has broad tissue tropism, including liver, muscle, lung, CNS, kidney, retinal, and pancreatic cells (Ahuja... is particularly well-suited for transduction of lung cells via airway administration (Halbert et al.,...identifying a binding receptor published this year (Dhungel et al., 2025)! It can transduce neurons for retrograde...AAV1, AAV8, AAV9 Kidney AAV8 Liver AAV8, AAV9 Lung AAV6 Pancreas AAV2, AAV8 Photoreceptor cells...1359–1368. https://doi.org/10.1089/hum.2008.123 Dhungel, B. P., Xu, H., Nagarajah, R., Vitale, J., Wong...Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2 Vectors. Journal of Virology...

...References Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DBT, Shmakov S, Makarova KS... Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA,..., Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F (2017) Nucleic...science.aam9321 Gootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F (2018) Multiplexed and portable...Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV ...Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F (...

...to their own devices. Chytrid fungi (Lillian Fritz-Laylin) Chytrid fungi are useful for the study of basic... basic evolution of fungi and eukaryotes and includes the fungus that is killing frogs all over the world...diverse plants, animals, microbes, viruses, and fungi for which these methods are presently unavailable...world. These are less well known fungi (they are not yeast or mushrooms) but there are over 1,000 species...

...possible TF-induced cellular states was generated (Joung et al., 2023). Fig. 1: Barcoded TF library...performed to map TFs to cell fates. (Adapted from Joung et al.)    This atlas linked TF expression to... nominated reference cell types.  (Adapted from Joung et al.)    Putting MORF to work Screens and ...addgene.org! References and Resources  References Joung J. et al., A transcription factor atlas of directed...186 (1) 209-229. doi: 10.1016/j.cell.2022.11.026 Joung J. et al., Genome-scale CRISPR Cas-9 knockout and...

...RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis in...model organism and to foster advanced training of young scientists in Latin America. The Zebrafish Book ...

...//doi.org/10.3390/cells8121510 Sung H, Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based...is quick, efficient, reliable and cost-effective (Sung, 2006). The sensor cell-based method uses specialized... mycoplasmas but also avoid other bacterial and fungal contamination. Proper PPE Since humans are the ... changed regularly as mycoplasma, bacteria, and fungus can reside in these containers. Clean up any media...

...stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their behavior. ...point where the temperature and humidity favors fungal growth. The ants bites down and locks their jaws...its muscles atrophy. After the ant’s death, the fungal fruiting bodies being to sprout out of the ant’...it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then poke out...

...variants derived from analysis of lung adenocarcinoma genomes (Lung Cancer Mutant Collection) or “pan-...variants and genes: High-throughput Phenotyping of Lung Cancer Somatic Mutations. Berger AH, Brooks AN, .... Cancer Cell, 2016. PubMed PMID: 27478040. The lung cancer mutant collection is described in this paper...

...Zhang Lab had described eSpCas9, while the Keith Joung Lab had developed SpCas9-HF1. Both of the enzymes...off-target activity relative to Cas9. Since then, the Joung and Jennifer Doudna Labs used targeted mutagenesis...also increased specificity. Most recently, the Jungjoon Lee Lab used directed evolution to generate Sniper-Cas9...29512652. PubMed Central PMCID: PMC5951633. Lee, Jungjoon K., et al. "Directed evolution of CRISPR-Cas9 ...

... Chun-Hyung Kim, Jung-Il Moon, Young-Gie Chung, Mi-Yoon Chang, Baek-Soo Han, Sanghyeok Ko, Eungi Yang,...

...and much more. Now, researchers from the Zhang, Joung, and Doudna labs have improved the on-target specificity... from the Zhang lab at the Broad Institute, the Joung lab at Harvard Medical School, and the Doudna Lab...testing of SpCas9-HF1 Kleinstiver et al and the Joung Lab similarly thought that if they could weaken ...structural knowledge, in collaboration with the Joung Lab, they used targeted mutagenesis to create HypaCas9...

...workshops at the Future of Research Symposium 2014, young scientists are acutely aware of these problems that...new approaches that address the need to train our young scientists for futures both inside and outside of...Paula Stephan, professor of economics at the Andrew Young School of Policy Studies at Georgia State University...diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the many different ...

...reference genomes, including animals, plants, bacteria, fungi, protists, and viruses. Developed by the Joachim...

...fluorescent protein mGold2 By Mike Lacy Get your sunglasses — these proteins are bright! Most current yellow...libraries By Mike Lacy Whether you’re planning on lounging poolside or swimming laps, Addgene has pooled ...engineering applications and is especially well-suited for lung cells via airway administration. AAV11 can transduce...reported to target skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have...

...linings of certain areas of the body, including the lung, the heart, and the abdomen. Long story short, when...frequently, ingested), it can get into the lining of the lungs or travel to one of the other areas of the body,...of other diseases, such as asthma, pneumonia, or lung cancer. As a result, mesothelioma is often misdiagnosed... from the government as more common cancers like lung (about 224,000 diagnosed this year) or breast cancer...

...Many thanks to our guest blogger Samantha Young. Samantha Young is a Medical Writer with a PhD in reproductive... post was contributed by guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has expanded... in scientific content.      References 1. Cho, Seung Woo, et al. "Analysis of off-target effects of CRISPR...

...proteins protect fragile samples during cryo-EM grid plunge freezing By Rachel Leeson Cryo-EM samples can be...glutaraldehyde. Adding RvLEAMshort to the sample before plunge freezing represents a convenient, effective approach...protectant for fragile samples during cryo-EM grid plunge freezing. bioRxiv 2024.02.06.579238. doi: https..., J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., … & Weissman, J. S. (2021...

...genotyping options. Figure reproduced from Tsai and Joung 2016 with permission. Even though progress has...experiments move closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique...specificities of CRISPR–Cas9 nucleases”
by Tsai and Joung 2016. Many thanks to our guest blogger Søren Hough...PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving the genome-wide specificities...