We narrowed to 88 results for: alp

1. CASTing Off for New Shores in Human Genome Editing

   Type

   Blog Post

   ...s41596-023-00927-3 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...

2. SunTag and Fluorescent Imaging

   Type

   Blog Post

   ... al. modified the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag...

3. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

   Type

   Blog Post

   ...right strain While common E. coli strains like DH5-alpha are recA-, some strains have been engineered to ...

4. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   ...neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298, and B.1.429 (Epsilon), nearly...

5. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

   Type

   Blog Post

   ...type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But while...

6. Scientists Map the SARS-CoV-2-Human Interaction Network

   Type

   Blog Post

   ...selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized...

7. 5 Great Apps for Lab Life

   Type

   Blog Post

   ...restriction site to a primer, you only need to skim the alphabet on the side of the screen to find the sequence...

8. Targeted Mutagenesis with EvolvR

   Type

   Blog Post

   ...PMID: 30253789. PubMed Central PMCID: PMC6154929. Halperin, Shakked O., et al. "CRISPR-guided DNA polymerases...

9. Viral Vectors 101: The AAV Retrograde Serotype

   Type

   Blog Post

   ...retrograde tracer) (Fig. 2). Viruses as neuronal scalpels Viruses can be injected into the brain and efficiently...

10. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    ...cleave DNA. Common lab E. coli K12 strains such as DH5alpha contain 3 methylases that recognize and methylate...

11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    ... the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty ...

12. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    ... LYS2 L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of a nitrogen source...

13. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

    ... plasmids could also be transformed into Alphaproteobacteira, Betaproteobacteria, and Gammaproteobacteria...

14. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    ...another excellent resource for research design is Kalpit Shah et al., “Do you know the sex of your cell?...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type

    Blog Post

    ... high-throughput screen for molecules affecting alpha-synuclein oligomerisation. Eastwood T, Baker K, ...

16. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type

    Blog Post

    ... the ligand binding domain of estrogen receptor alpha (Esr1). Mice are fed or injected with tamoxifen,...

17. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    ...Virus Many virus experiments involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment...

18. Hot Plasmids February 2024

    Type

    Blog Post

    ...versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    ...Sickle Cells Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far...

20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type

    Blog Post

    ...flies before, get yourself a copy of Fly Pushing by Ralph J. Greenspan. It’s a fantastic book for newbies ...