We narrowed to 4 results for: alp

1. Transfection for Recombinant Antibodies

   Type

   Protocol

   ...use. Reagent Preparation 100 mM Valproic Acid Dissolve 2.88 g valproic acid in 200 mL deionized water ..., VWR 430770 Trypan Blue, Thermo Fisher T10282 Valproic acid sodium salt, Sigma Aldrich P4543-10G Polyethylenimine...flask to the incubator. Section 3: BCD Feed and valproic acid supplementation During the 24–144 h post-...post-transfection, supplement the flask with 3.75 mM valproic acid. Example feeding schedule: Thursday: Transfect...BCD Feed. Monday (96 h post-transfection): Add valproic acid to 3.75 mM, add 4% BCD Feed. Tuesday (120...

2. Protocol - pLKO.1 – TRC Cloning Vector

   Type

   Protocol

   ...NEB #M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012 Qiaquick gel...Transform 2 μL of ligation mix into 25 μL competent DH5 alpha cells, following manufacturer’s protocol. Plate ...

3. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

   Type

   Protocol

   ...ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total DNA (<1ng...

4. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total DNA (<1ng) ...