RNA Extraction Without A Kit
Type
Blog Post
...selectively precipitate RNA, but not DNA and proteins. Lastly, using a DNase (there are several DNase enzyme ...
We narrowed to 50 results for: astl
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Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post...CRISPR systems for high-throughput screening are vastly useful for mapping the combinatorial genetics that... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...interaction with the BirA* fused bait proteins ceases. Lastly, Split-BioID and BioID identify proteins that interact... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...recent advancements in various AAV technologies have vastly improved AAVs’ potential to become ideal vectors... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...materials and assignments. 9) Citizen science projects Lastly, if you'd like to directly involve the public in... -
Tips for a 1st time AAV user (by a Rookie AAV user)
TypeBlog Post...can be cooled and the two phases mixed together. Lastly, the PEG solution needs to be sterilized, either... -
The AAVantages of AAV in CRISPR Screens
TypeBlog Post...while many more have been engineered in labs. This vastly increases the possibilities when designing screens... -
Pooled Library Amplifications
TypeBlog Post...any of these options work! Selecting your media Lastly, you need to decide between growing your library... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post...base pair may not be enough to prevent re-binding. Lastly, the sequence inserted by HDR may inadvertently... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...contamination. We do not normally use antibiotics. Lastly, and perhaps obviously, it is important to have... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...than the standard spCas9 (Harrington et al. 2017). Lastly, as with any scientific research, choice of a technique... -
Plasmids 101: Colony PCR
TypeBlog Post...You can use this plate to start liquid cultures. Lastly, you can start small overnight liquid cultures ... -
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design
TypeBlog Post...similar on-targeting cutting as full-length gRNAs Lastly, the effects of guide length on off-target cuts... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog Post...mixer, they meet other Mentors and Singletons, and vastly expand their networks as they naturally find people... -
Hot Plasmids: Fall 2024
TypeBlog Post...induced to cause disruption of the target gene. Lastly, curing the mCherry-sgRNA plasmid leaves an edited... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...when it is perceived as being done in the dark. Lastly, data is our raw product. If we don’t take interest... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...reasonable amount of time, but not so many that they vastly outnumber the virus particles. Incubate the cells... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...HDR event, then the second HDR event can’t occur. Lastly, depending on its design, donor A may be capable... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...the subject after I’ve left the room. Microphone: Lastly, I needed to figure out a microphone situation.... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post... performed on cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule...