We narrowed to 46 results for: astl

1. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   Published

   Oct. 10, 2019, 12:41 p.m.

   ...signal to noise ratio five fold over the 1xHA tag.  Lastly, the researchers found that the HA frankenbody ...the ability to bind nascent HA tagged proteins.  Lastly, the HA frankenbody can be used for imaging in ...

2. Five Popular Model Organisms, Part 2

   Type

   Blog Post

   Published

   Sept. 29, 2020, 1:15 p.m.

   ...modification tools and methods such as CRISPR have vastly improved scientists’ ability to use rats as disease...first, advances in axolotl genome sequencing have vastly expanded axolotl research. One of the most significant...

3. Antibodies 101: Designing Your First Flow Panel

   Type

   Blog Post

   Published

   May 28, 2024, 1:45 p.m.

   ...separately detect these markers in your analysis. Lastly, there are fixable dye stains that react with free...will now exclude FITC from the rest of our panel. Lastly, we want to detect the expression of our protein...

4. Using AAV for Neuronal Tracing

   Type

   Blog Post

   Published

   Aug. 9, 2018, 1:04 p.m.

   ..., and bidirectional transport for AAV1, -8, -9 (Castle et al., 2014). Differences between AAV serotypes...PMID: 25555356. PubMed Central PMCID: PMC4324159. Castle, M.J., Gershenson, Z.T., Giles, A.R., Holzbaur,...

5. We’re Going Viral This Week!

   Type

   Blog Post

   Published

   Oct. 14, 2019, 1 p.m.

   ...increase AAV2 yields and we’re eager to share more! Lastly, we’ll be at SfN2019. If you’re attending SfN2019...

6. Bringing Sustainable Practices to the Lab: Easy Action Items

   Type

   Blog Post

   Published

   April 22, 2019, 12:26 p.m.

   ... do the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term...

7. Science Communication for Everyone

   Type

   Blog Post

   Published

   April 16, 2019, 1:05 p.m.

   ...and doctors with their questions and comments.  Lastly, we are planning to launch an e-magazine this year...

8. Understanding GPCR Signaling Using TRUPATH

   Type

   Blog Post

   Published

   Dec. 14, 2020, 2:15 p.m.

   ...effectiveness of using TRUPATH as a screening technique. Lastly, the activity of both antagonists and inverse-agonists...

9. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

   Type

   Blog Post

   Published

   Sept. 3, 2019, 1:15 p.m.

   ...interest to create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a...

10. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    Published

    May 21, 2019, 12:10 p.m.

    ...staining, a popular method to observe apoptotic cells. Lastly, the Shu Lab demonstrated that the FlipGFP-based...

11. 5 Great Apps for Lab Life

    Type

    Blog Post

    Published

    Sept. 22, 2016, 2:30 p.m.

    ... find the sequence for your enzyme of interest. Lastly, the Tm calculator helps you set up an optimal ...

12. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    Published

    Feb. 2, 2021, 2:15 p.m.

    ... letter that is customized for each internship. Lastly, have your resume and cover letter revised by your...

13. A Quick Guide to a Career in Software Product Management

    Type

    Blog Post

    Published

    Nov. 24, 2020, 2:15 p.m.

    ...asset to the scientists as well as the developers. Lastly, you’re going to need to be a problem-solver. A...

14. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...through the presence of a fluorescent reporter. Lastly, once a mutant is identified, the PB transposase...

15. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ...ability to knock out multiple genes in parallel. Lastly, this Cas12a-based screening method was used to...

16. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    Published

    May 9, 2017, 2:12 p.m.

    ...of a crRNA array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes...

17. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type

    Blog Post

    Published

    Feb. 19, 2019, 1:36 p.m.

    ...Information Systems Audit and Control Association. Lastly, Carl also serves on the Board of Advisors and ...

18. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...opposite strands. This “double nickase” strategy vastly reduces breaks and mutations at off-target sites...

19. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...selectively precipitate RNA, but not DNA and proteins. Lastly, using a DNase (there are several DNase enzyme ...

20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...CRISPR systems for high-throughput screening are vastly useful for mapping the combinatorial genetics that...