We narrowed to 109 results for: atr;

1. Sensing Neuronal Dopamine

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   Blog Post

   ... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   ...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...

3. In Vivo Biotinylation of Bacterial Fusion Proteins

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   Blog Post

   ... have been immobilized on a reduced glutathione matrix. The advantage of a fusion protein is the efficient...available. Factor Xa loves calcium, but metal-chelate matrices, not so much. Maybe a calcium-independent proteinase...

4. What Do I Do Now? Academic v. Non-Academic Career Decisions

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   ... notice or choice Always working on a team/in a matrix - can be frustrating for solo flyers Other people... years—a short and exciting ride Complicated matrix structures are common Small integrated teams, ...

5. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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   ...tuberculosis Dr. Patricia Sheen, Universidad Peruana Cayetano Heredia, Peru Patricia Sheen, Professor ...

6. Rett Syndrome

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   ...in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in a new window)...Methyl-CpG binding protein 2. Am J Med Genet B Neuropsychiatr Genet . 180, 55–67. PMID: 30536762 Shahbazian...diagnosticians and risk factors for late diagnosis. Pediatr Neurol . 52, 585-591.e2. PMID: 25801175 Tillotson...

7. Deisseroth INTRSECT Collection

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   ...S, Sienna AC, Tepler S, Poulin JF, Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron ...Ramakrishnan C, Chan CS, Dombeck DA, Deisseroth K, Awatramani R. 2018. Mapping projections of molecularly defined...hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PubMed (Link opens in a new ...

8. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...et al. "DNA-guided genome editing using the Natronobacterium gregoryi Argonaute." Nature biotechnology ...enzyme." DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development...

9. Plasmids 101: Optimizing Plasmid Yields

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   ...volume of lysate passed over the binding matrix could clog the matrix when performing filter based plasmid...

10. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...Last updated Sep 30, 2020 by Marcy Patrick. CRISPR technology has been widely adopted for genome editing...for creating the Expression Systems table. Marcy Patrick contributed to the writing of this post. Additional...

11. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...default scoring matrix BLOSUM62, but you may want to check the description of the other matrices to see if ...

12. Deciphering the Mysteries of Behavior with Viral Vectors

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    ...levels in general, or anxiety associated with psychiatric diseases such as schizophrenia or bipolar disorder...the BDNF hypothesis of depression?." Molecular psychiatry 12.12 (2007): 1079. PubMed PMID: 17700574. Hare...

13. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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    ... be captured on streptavidin affinity beads or matrices. The bait protein can even target BirA* to specific...identify the proteomic map of the human mitochondrial matrix (Rhee et al., 2013) and determine the topology ...

14. Multiple Plasmids at a Low Price: Kits from Addgene

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    ...study a matrix metalloproteinase: López-Pelegrín, Mar, et al. "A novel mechanism of latency in matrix metalloproteinases...

15. Fluorescent Protein Guide: Biosensors

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    ...green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green- and...Recording of Zn(2+) Dynamics in the Mitochondrial Matrix and Intermembrane Space with the GZnP2 Sensor. ...Nat Methods. 2023 Sep;20(9):1426-1436. Tommaso Patriarchi Norepinephrine GRAB_NE family of GPCR activation-based... Nat Methods. 2022 Feb;19(2):231-241. Tommaso Patriarchi Serotonin Red and green serotonin sensors GRAB...

16. A Guide to Getting Started in Undergrad Research

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    ...blogger Eleanor Wang, a Research Specialist in Patrick Hsu's lab at UC Berkeley. Most of the scientists...Eleanor Wang is currently a Research Specialist in Patrick Hsu’s lab at UC Berkeley. She will be starting ...

17. Selecting Your Plasmid Purification Kit

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    ... is a silica matrix fixed in a column and you flow lysate and buffers through the matrix, usually by centrifugation...

18. TALENs for Endogenous Zebrafish Genes

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    ...TCTTCTTGTGCTCTTATTggcaggtacctggtgacTCACCTTATGGGCGCTGA matrixmetallopeptidase15 (membrane-inserted) TAL3484 & TAL3485 ...TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA matrixmetalloproteinase14a (membrane-inserted) TAL3486 & TAL3487...

19. Biosensor AAV Preps

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    ...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...

20. Antibody Guide

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    ...tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule may be a..., cells have most or all of their extracellular matrix removed. Again, the signaling molecule may be a...