...Nicola Patron is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like...that go on between plants and their pathogens. As Patron explains it, her work has always been focused on...Modular Cloning (MoClo) Kit. What does it include? Patron: Sainsbury has a mandate to share and educate and...Addgene: Will the MoClo tools work in any plant? Patron: The MoClo kit is entirely for dicots, not grasses...tips for those looking to get started with MoClo? Patron: The parts will be familiar to most plant scientists...there other tools you are working on for plants? Patron: Our lab and others at the Sainsbury are working... of engaging about science in that way for you? Patron: I started my Twitter account recently inspired...

...prevalence of spinal muscular atrophy (SMA)." European Journal of Paediatric Neurology 19 (2015): S64-S65...afflictions. Elia Di Schiavi - Spinal muscular atrophy Dr. Elia Di Schiavi is a Research Scientist at ...Naples, Italy. His group works on spinal muscular atrophy, a disease in which disruption of the smn1 gene...the smn1 gene is known to cause spinal muscular atrophy, the molecular mechanisms behind the disease are...restore motor neuron function in spinal muscular atrophy patients. Noushin Nabavi - Peritoneal Mesothelioma...

...anode/iblot gel matrix, the blotting membrane, the gel, a copper cathode/iblot gel matrix, and filter paper...remain intact and the protein is separated through a matrix by charge. In a denaturing western, the protein...smaller molecules moving more quickly through the matrix. For the purpose of this blog, we will be focusing...proteins encounter less resistance in the acrylamide matrix and migrate faster through the gel. For better ...

... and were only available as green probes. The Patriarchi Lab aimed to overcome these limitations by developing...researchers looking to study norepinephrine, the Patriarchi lab continues to work on further improvements...nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References...Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for...

... do not require any punctures. Intratracheal instillation Intratracheal instillation is used to deliver...Intranasal instillation is very similar to its intratracheal counterpart, as both procedures target the lungs...Anesthesia Spinal cord 30 up to 0.01 mL Expert Intratracheal Anesthesia Trachea 20–23 (tubing) < 0.07 mL ...

...communities. Many thanks to our guest blogger Katrina Armstrong. Katrina Armstrong is a Neurophysiology Msc Student...This post was contributed by guest blogger Katrina Armstrong, a Neurophysiology Msc Student at the University...serotonin in movement. Follow her on twitter @katrinaboards.   References 1. Russell, David W. "AAV vectors...

...Kamenyeva et al. 2015)? How do blood monocytes patrol blood vessels during immune responses (Auffray ...and tissues by a population of monocytes with patrolling behavior." Science 317.5838 (2007): 666-670. ...17673663. 14. Finsterbusch, Michaela, et al. "Patrolling monocytes promote intravascular neutrophil activation... PubMed PMCID: PMC5024581. 15. Celli, Susanna, Béatrice Breart, and Philippe Bousso. "Intravital two-photon...

...Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered...Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick Will You Be My Mentor? Finding and Asking for...

...guest blogger Patrick Miller-Rhodes from the University of Rochester Medical Center! Patrick is equal parts...This post was contributed by Patrick Miller-Rhodes from the University of Rochester Medical Center.  ...

...guest blogger Patrick Miller-Rhodes from the University of Rochester Medical Center. Patrick Miller-Rhodes...This post was contributed by Patrick Miller-Rhodes, a Ruth L. Kirschstein NRSA Predoctoral Fellow at ...

...) -  perhaps you’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project. Gymnotiformes... aspects of cognition. Spiralians - Crepidula atrasolea (Jonathan Henry) This black footed slipper snail...bottleneck. New gif for #MolluscMonday: #Crepidula atrasolea juvenile, light microscope with polarizing filter...

...times. Many thanks to our guest blogger, Katrin Michel! Katrin Michel is a Postdoctoral Researcher at MIT...This post was contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful...

... that they can be imported to the mitochondrial matrix. Gottlieb’s lab has used an inducible MitoTimer...Addgene depositors Sylvestre Marillonnet and Nicola Patron have assembled two collections of standardized ...general eukaryotic multigene constructs, while the Patron Lab MoClo Plant Parts kit contains modules specific.../sb4001504. For further reading about Nicola Patron's MoClo kit and her plant synbio research, read ...

...AAV9 Biosensors Patriarchi New viral service pAAV_hSyn1_nLightR AAV9 Biosensors Patriarchi New viral service...

...their jaws into a leaf, and dies as its muscles atrophy. After the ant’s death, the fungal fruiting bodies...blood out. Once the blood is depleted, the tongue atrophies and falls off. The louse remains becoming the ...

... tool to detect and image DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate...PMID: 4403305. PubMed Central PMCID: PMC426888. Patriarchi, Tommaso, et al. "Ultrafast neuronal imaging ...

... notice or choice Always working on a team/in a matrix - can be frustrating for solo flyers Other people... years—a short and exciting ride Complicated matrix structures are common Small integrated teams, ...

... have been immobilized on a reduced glutathione matrix. The advantage of a fusion protein is the efficient...available. Factor Xa loves calcium, but metal-chelate matrices, not so much. Maybe a calcium-independent proteinase...

...gene-editing protein - a DNA-cleaving Argonaute from Natronobacterium gregoryi, or NgAgo for short. Addgene has ... Han. “DNA-guided genome editing using the Natronobacterium gregoryi Argonaute.” Nat Biotechnol. Epub ...

...tuberculosis Dr. Patricia Sheen, Universidad Peruana Cayetano Heredia, Peru Patricia Sheen, Professor ...