Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 41 results for: biotin

Showing: 21 - 40 of 41 results

  1. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...version of the promiscuous biotin ligase used in their popular biotinylation method for identifying protein-protein...method uses a bait protein fused to a biotin ligase to biotinylate proximate proteins in the cell. These...These candidate proteins can be enriched via biotin pull-down methods and identified using mass spectrometry...selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate proteins...Acids Res. 2016. PubMed PMID: 26969735 Updated biotinylation tools to identify proximate proteins The Roux...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    ...binary system for cell- and compartment-specific biotin labeling in zebrafish. pGCS Vector Kit - Hai-Ning...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...captures the streptavidin portion of the probe via biotin. The fluorescent portion of the probe that is cut...target sequence is amplified by PCR/RPA using biotinylated primers. These are then immobilized with streptavidin...that contain streptavidin which can bind to the biotinylated targets. They also tried to confirm target binding...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...counterpart label nearby proteins with biotin. The biotinylated proteins can then be isolated and identified...

  10. CRISPR Guide

    Type
    Collection
    ...by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing...system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system...co-expressing BirA biotin ligase. Whatever the chosen epitope tag, the locus is subsequently isolated ...In situ capture of chromatin interactions by biotinylated DCAS9. Cell . 170 (5), 1028-1043.e19. PMID: ...

  11. CRISPR Guide

    Type
    Guide
    ...by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing...system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system...co-expressing BirA biotin ligase. Whatever the chosen epitope tag, the locus is subsequently isolated ...In situ capture of chromatin interactions by biotinylated DCAS9. Cell . 170 (5), 1028-1043.e19. PMID: ...

  13. Molecular Biology Reference

    Type
    Guide
    ...Xpress DLDDDDK or DLYDDDDK Thrombin LVPRGS BAD (Biotin Acceptor Domain) GLNDIFEAQKIEWHE Factor Xa IEGR...

  15. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Generation Toolbox! Starting with libraries and a biotinylated (for purification purposes) protein target of...process, displayed sybodies that bind to the biotinylated target protein can be recovered for the subsequent...plate to capture Myc-tagged sybodies. Then, the biotinylated target protein is added and after a few washes...

  16. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...bi-directionality. For predominately anterograde tracing, biotinylated dextran amines (Veenman et al., 1992) or fluorescent...Veenman, C.L., Reiner, A., and Honig, M.G. (1992). Biotinylated dextran as an anterograde tracer for single-...

  18. Plasmids 101: Protein tags

    Type
    Blog Post
    ...Combo Consists of a bacterially derived in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine...

  19. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher linkers and spacer proteins...
Showing: 21 - 40 of 41 results