We narrowed to 42 results for: biotin

...version of the promiscuous biotin ligase used in their popular biotinylation method for identifying protein-protein...method uses a bait protein fused to a biotin ligase to biotinylate proximate proteins in the cell. These...These candidate proteins can be enriched via biotin pull-down methods and identified using mass spectrometry...selective targeting of fusion proteins, requires less biotin, and shows enhanced labeling of proximate proteins...Acids Res. 2016. PubMed PMID: 26969735 Updated biotinylation tools to identify proximate proteins The Roux...

... Engineered ligases (e.g., lipoic acid ligase, biotin ligase, phosphopantetheinyltransferase) – These...

...binary system for cell- and compartment-specific biotin labeling in zebrafish. pGCS Vector Kit - Hai-Ning...

...molecular biology studies. For example the E. coli biotin ligase, BirA, has been used to identify and study...

...captures the streptavidin portion of the probe via biotin. The fluorescent portion of the probe that is cut...target sequence is amplified by PCR/RPA using biotinylated primers. These are then immobilized with streptavidin...that contain streptavidin which can bind to the biotinylated targets. They also tried to confirm target binding...

...proteins with a membrane-impermeable biotin substrate (Figure 1). Biotinylated proteins were isolated, identified...

...counterpart label nearby proteins with biotin. The biotinylated proteins can then be isolated and identified...

...soon) as well as epitope tags like His, Flag, and Biotin. The antibodies developed by IPI are extensively...

...your protein, such as with substrate-linked PEG, biotin, or magnetic beads. In one innovative example, ...

...Protein-protein interaction Proximity-dependent biotin identification (BioID) MCS-BioID2-HA or myc-BioID2...

...by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing...system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system...co-expressing BirA biotin ligase. Whatever the chosen epitope tag, the locus is subsequently isolated ...In situ capture of chromatin interactions by biotinylated DCAS9. Cell . 170 (5), 1028-1043.e19. PMID: ...

...by the Jian Xu lab , utilizes biotin tagging of dCas9 by fusing a biotin acceptor site to dCas9 and co-expressing...system can use common tags, like 3xFLAG-tag, PA, and biotin tags, or an anti-Cas9 antibody. The CAPTURE system...co-expressing BirA biotin ligase. Whatever the chosen epitope tag, the locus is subsequently isolated ...In situ capture of chromatin interactions by biotinylated DCAS9. Cell . 170 (5), 1028-1043.e19. PMID: ...

...associated proteins or RNAs through ChIP (enChIP) or biotin-streptavidin pulldown (CAPTURE.) Tag : Multiple...

...Xpress DLDDDDK or DLYDDDDK Thrombin LVPRGS BAD (Biotin Acceptor Domain) GLNDIFEAQKIEWHE Factor Xa IEGR...

...streptavidin-binding peptide (SBP). The addition of biotin disrupts this interaction and thus triggers a synchronous...

...Generation Toolbox! Starting with libraries and a biotinylated (for purification purposes) protein target of...process, displayed sybodies that bind to the biotinylated target protein can be recovered for the subsequent...plate to capture Myc-tagged sybodies. Then, the biotinylated target protein is added and after a few washes...

...bi-directionality. For predominately anterograde tracing, biotinylated dextran amines (Veenman et al., 1992) or fluorescent...Veenman, C.L., Reiner, A., and Honig, M.G. (1992). Biotinylated dextran as an anterograde tracer for single-...

..., the receptor fused to APEX2 catalyzes the biotinylation of proteins near the GPCR. These proteins are...

...Combo Consists of a bacterially derived in-vivo biotinylation signaling peptide (Bio), flanked by hexahistidine...

...streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher linkers and spacer proteins...