We narrowed to 70 results for: calcium

1. In Vivo Biotinylation of Bacterial Fusion Proteins

   Type

   Blog Post

   .... Factor Xa loves calcium, but metal-chelate matrices, not so much. Maybe a calcium-independent proteinase...

2. Five Popular Model Organisms

   Type

   Blog Post

   ...Andrew Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid...plasmid that expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are ...PMC5349099. Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular resolution in freely behaving...

3. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

   Type

   Blog Post

   ...manipulation in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane ...and physical methods in the lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize...

4. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

   Type

   Blog Post

   ...will image these neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine ...enable multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure...

5. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   ... and ways to navigate it, we’ll search for the calcium biosensor, GCaMP, and click through to the plasmid...distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide you’d really...

6. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

7. Twenty Years of Sharing: Addgene's Viral Vector Service

   Type

   Blog Post

   ... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

8. Fluorescent Proteins 101: History of Fluorescent Proteins

   Type

   Blog Post

   ...aequorin, gives off a faint blue light when it binds calcium ions, and the second, which we now call GFP, absorbs...

9. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

   Type

   Blog Post

   ...GCaMP5 and GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year ...

10. AAV Viral Preps

    Type

    Collection

    ...Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls...

11. Hot Plasmids and Viral Preps - July 2021

    Type

    Blog Post

    ...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

12. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type

    Blog Post

    ...targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity...

13. Sensing Neuronal Dopamine

    Type

    Blog Post

    ... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...

14. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type

    Blog Post

    .... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...

15. Brain Initiative Collection

    Type

    Collection

    ...-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1 pAAV-hSynapsin1...135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter...pAAV-syn-FLEX-axon-jYCaMP1s Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter...Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter...

16. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    ... previous implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into...

17. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    ...modulate electricity. If you could selectively let in calcium or some other specific ion that would be interesting...

18. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    ...cells that are not excitable but responsive to calcium. We’d love to expand our cancer and immunology ...

19. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type

    Blog Post

    ... of serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured...

20. Optogenetics Guide

    Type

    Guide

    ...-encoded reporters of molecular signals; e.g., calcium indicators In this overview, we will focus on the... truncated forms of cytosolic STIM1 to control calcium channels ~488 LOV domains LOV-based Light-oxygen-voltage-sensing...light-controlled molecules. ~450–495 BACCS light-activated calcium channel switch iLID Improved light-inducible dimers...