...Andrew Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid...plasmid that expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are ...PMC5349099. Nguyen, Jeffrey P., et al. "Whole-brain calcium imaging with cellular resolution in freely behaving...

...manipulation in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane ...and physical methods in the lab. Chemicals like calcium phosphate and diethylaminoehtyl (DEAE)-dextra neutralize...

...will image these neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine ...enable multiplexed imaging of neurotransmitter, calcium, and membrane voltage,” says Tian. Figure...

...vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded our...

... and ways to navigate it, we’ll search for the calcium biosensor, GCaMP, and click through to the plasmid...distribution. If your experiment involves delivering a calcium biosensor using AAV, you might decide you’d really...

... ligands (Figure 2). Biosensors, which include calcium and voltage sensors, are the broadest category ... vectors available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization...

...aequorin, gives off a faint blue light when it binds calcium ions, and the second, which we now call GFP, absorbs...

...GCaMP5 and GCaMP6 series of genetically encoded calcium indicators were the hot technology of the year ...

...Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls...

...and AAVrg vectors. New red-shifted INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments...

...targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity...

... animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1 sensor is...

.... The non-specific cation channel ChR2 and the calcium channel LiGluR both cause ion movement that results...

...-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1 pAAV-hSynapsin1...135420-AAV1 pAAV-syn-FLEX-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter...pAAV-syn-FLEX-axon-jYCaMP1s Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter...Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s Yellow protein calcium sensor expressed under human Synapsin1 promoter...

... previous implementation of optogenetic tools, calcium sensors, and other common neuroscience tools into...

...modulate electricity. If you could selectively let in calcium or some other specific ion that would be interesting...

...cells that are not excitable but responsive to calcium. We’d love to expand our cancer and immunology ...

... of serotonergic neurons (measured using a red calcium sensor) leads to the release of serotonin (measured...

...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

... Lysine (D123K), lowers biosensor affinity for calcium.”  Primary Vector Type The vector type should...