We narrowed to 110 results for: chm
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TypeBlog Post... cells. Moreover, they optimized the specific attachment sites via sequence engineering, enabling even...Eleonora I. Ioannidi, Matthew T. N. Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger...
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Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...called BLESS (direct in situ breaks labeling, enrichment on streptavidin and next-generation sequencing.../biot.201400046 Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin ... -
Hot Plasmids - November 2023
TypeBlog Post...one enzyme’s substrate, then the other, a dual-enrichment and mass spectrometry workflow identifies proteins...and APEX2 at different cellular locations. C) Enrichment of dual-labeled proteins identifies proteins ... -
Plasmids 101: Codon usage bias
TypeBlog Post...regions a chance to fold properly. For example Pechmann and Frydman found that tracts of non-optimal codons...20506237. PubMed Central PMCID: PMC2970903. 6. Pechmann, Sebastian, and Judith Frydman. "Evolutionary ... -
Plasmids 101: Broad Host Range Plasmids
TypeBlog Post...RK2 (IncP), RSF1010 (IncQ), and pSa (IncW) (Schmidhauser et al. 1988; Lale et al 2011; Jain and Srivastava... 326–332. https://doi.org/10.1002/bit.22695 Schmidhauser, T. J., Ditta, G., & Helinski, D. R. (1988).... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002). How to pick a split AAV method...1–12 . https://doi.org/10.1089/hgtb.2015.140 Frischmeyer PA (2002) An mRNA Surveillance Mechanism That... -
The Fluorescent Vegetables in Aptamer Soup
TypeBlog Post...Plasmids 101: Aptamer Fluorophores describes the enrichment process used to evaluate oligonucleotides for...systematic evolution of ligands by exponential enrichment (SELEX). After 4-6 rounds of SELEX, the RNA pool... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...Plasmid #67178) for use in the system outlined in Schmid-Burgk, J.L., et al (2). In this post, I’ll cover... 22894855. PubMed Central PMCID: PMC3501149. 2. Schmid, J.L., et al. (2016) CRISPaint allows modular base-specific... -
CRISPR Guide
TypeCollection...A., Waldhauer, M. C., Börner, K., Fakhiri, J., Schmelas, C., Dietz, L., Grimm, D., Correia, B. E., Eils...Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M.... 31189177 Strecker, J., Ladha, A., Gardner, Z., Schmid-Burgk, J. L., Makarova, K. S., Koonin, E. V., &...: 34478496 Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...higher specificity, increased versatility in probe attachment, and compatibility with far-red and near-infrared...References Allikalt, A., Purkayastha, N., Flad, K., Schmidt, M. F., Tabor, A., Gmeiner, P., Hübner, H., & Weikert... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M....024-07259-6 Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L... -
Immunology Research Plasmids and Resources
TypeCollection...leukocyte cell-derived chemotaxin 2 MGC126628, chm-II, chm2 LTB4R leukotriene B4 receptor BLT1, BLTR, CMKRL1...leukocyte cell-derived chemotaxin 2 MGC126628, chm-II, chm2 LEFTY1 left-right determination factor 1 LEFTB...PIG4, SAA, TP53I4 SAA2 serum amyloid A2 - SBDS Shwachman-Bodian-Diamond syndrome CGI-97, FLJ10917, SDS,... -
CRISPR Pooled gRNA Libraries
TypeCollection...CRISPR Knockout Library 104861 Knockout Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein ...Pooled Library (Brunello) 223064 Knockout Human Schmid-Burgk 3rd 4 76,441 No available items found to ... -
Using AAV for Neuronal Tracing
TypeBlog Post... or fluorescent molecules such as Fluoro-Gold (Schmued and Fallon, 1986) are used. To facilitate cellular...PMID: 22418061. PubMed Central PMCID: PMC3381869. Schmued L.C., and Fallon J.H. (1986). Fluoro-Gold: a new... -
Validated gRNA Sequences
TypeCollection...25490046 Schmitt-Ulms Prnp M. musculus TTGGCCCCATCCACCGCCAT 61856 cut S. pyogenes 25490046 Schmitt-Ulms Pten... -
CRISPR Guide
TypeGuide...A., Waldhauer, M. C., Börner, K., Fakhiri, J., Schmelas, C., Dietz, L., Grimm, D., Correia, B. E., Eils...Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M.... 31189177 Strecker, J., Ladha, A., Gardner, Z., Schmid-Burgk, J. L., Makarova, K. S., Koonin, E. V., &...: 34478496 Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog Post... systems Proof of Concept These systems were benchmarked on positive control genes in CD4+ and CD8+ T ... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...How to Design Your gRNA Use the CRISPR Software Matchmaker Browse Alll of Our CRISPR Posts Additional Resources... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...from webpage clippings, photographs, and file attachments. Organize your notes into notebooks, create checklists... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post...first mapping of intrachromosomal loci, a major benchmark in characterizing the 4D nucleome. Future modifications...