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We narrowed to 200 results for: ckb

Showing: 21 - 40 of 200 results

  1. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...Plasmid A (encoding shRNA against human Gene X in backbone Y) was transfected into human cells. From this...control vector would be Plasmid A sans shRNA, or backbone Y alone. The empty vector control allows you to... vector control shows any effect of the vector/backbone itself on gene expression in your target cells... this experiment could be an shRNA—in the same backbone as Plasmid A—that does not target any mammalian...

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...retroviral backbones. On some occasions, shRNAs are transiently expressed using either AAV backbones or species-specific...Designing the shRNA sequence Choosing the plasmid backbone Choosing the promoter Mature siRNAs typically...Created on BioRender.com. When it comes to the backbone, shRNAs are most often expressed constitutively...

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...cloning my target spacers into the PX330 vector backbone? A2: There is no need to add the NGG PAM sequence...designing oligos for cloning my target sequence into a backbone that uses the human U6 promoter to drive expression...plasmid having a targeting spacer cloned into the backbone (pX330 or PX335, depending if you want to use ...quickly check if the cloning of my oligo into a backbone like PX330 worked? A12: You can quickly check ...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... resistances, restriction enzymes and plasmid backbones. pUCXKT contains a nonfunctional truncated version...site in pUCXKT, removing the stop codons during backbone digestion and providing the missing codons for... both kanamycin and ampicillin (from the pUC19 backbone). *False positives can occur in this system if...lentivirus production Finding a lentiviral vector backbone with just the right combination of promoter, cloning...create the pCell-Free vectors, Gateway-compatible backbones which enable cell-free expression of proteins ...originating from cryptic promoters in the plasmid backbone (Giannaks et al 2003, Bert et al 2000). In 2000...

  5. An Addgene Summer Internship

    Type
    Blog Post
    ... cloning the Syntaxin3 gene in a pLenti vector backbone. During those five weeks, I worked with my mentors...had successfully incorporated Syntaxin3 into its backbone. Once we had gotten our results back from the ...successful clone of the Syntaxin3 in the pLenti backbone! Once everything was confirmed, I worked on my...

  6. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...particular vector by excising the insert from the backbone. To do this, you'll use enzymes with restriction...to know both the approximate size of the vector backbone as well as the predicted size of the insert. You...bands at 6kb and 1.2kb (red box), matching the backbone and insert, respectively. The results on the gel...

  7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    ... NGS result to a reference sequence to confirm backbone elements.   Confirming the gene/insert by aligning...NGS result to a reference sequence like a known backbone sequence. We’re not necessarily expecting a perfect...mismatches in the origin of replication or other common backbone elements. Since we’ve successfully grown the plasmid...

  9. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...species. These plasmids all have a PR-T4 vector backbone, which is a modified version of L440. This vector...cloning to insert the cDNA into the desired plasmid backbone. The Sánchez lab plasmids allow students and new... gene of interest into the empty PR-T4P vector backbone. The empty vector PLL4, which is similar to PR-T4P...

  10. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    ... of the essence. This method requires a vector backbone containing a topoisomerase I cut site, toposisomerase...the topoisomerase I generated overhangs in the backbone. Once combined, the cut and ligation processes...out protocols for plasmid cloning Find vector backbones Read our molecular biology reference ...

  11. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...expressed from different plasmid backbones. They noted that backbone differences such as the order, or...used to assess how different lentiviral plasmid backbone elements impact viral titer and to compare different...

  15. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...parental antibody (blue, rat) are cloned into a backbone expressing the constant regions from an alternative... a specific antibody are cloned into a plasmid backbone containing all constant regions, just the heavy...

  16. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...Check Out Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids...

  19. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...create an amplicon that  was cloned into a plasmid backbone for expression of the mAb heavy and light chain...modify the CH encoding sequence within the plasmid backbone to generate R-mAbs of different subclasses, including...
Showing: 21 - 40 of 200 results