Cloning
Type
Guide
...Techniques You may also like... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme...compatible restriction enzymes, that cut your insert or backbone at only the desired location for your cloning ...putting it under a variety of promoters and into backbones with different selection cassettes). Although ...disadvantage of TOPO® cloning is that very few plasmid backbones are available TOPO® ready, and it is not feasible...overhangs can be efficiently ligated into any plasmid backbone independent of how it was originally designed ...