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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8 results for: ckb

Showing: 1 - 8 of 8 results

  1. Adenovirus Guide

    Type
    Guide
    ...pAdEasy-1 Adenoviral backbone plasmid that lacks E1 and E3. pAdEasy-2 Adenoviral backbone plasmid that lacks...transgene of interest and both ITRs. Adenoviral backbone plasmid (e.g. pAdEasy) — containing the adenoviral...containing pAdEasy. pAdEasy is a ~33 kb adenoviral backbone plasmid containing the adenoviral genes necessary...production. The shuttle plasmid and the adenoviral backbone plasmid have matching left and right homology ...recombination of the transgene into the adenoviral backbone plasmid. One can also co-transform standard BJ5183...transgene has been inserted into the adenoviral backbone plasmid, and that other patterns of recombination...equivalent across multiple viruses. The adenoviral backbone plasmid pAdEasy-1 (Addgene #16400) is suitable...

  2. Cloning

    Type
    Guide
    ...Techniques You may also like... Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme...compatible restriction enzymes, that cut your insert or backbone at only the desired location for your cloning ...putting it under a variety of promoters and into backbones with different selection cassettes). Although ...disadvantage of TOPO® cloning is that very few plasmid backbones are available TOPO® ready, and it is not feasible...overhangs can be efficiently ligated into any plasmid backbone independent of how it was originally designed ...

  3. Molecular Biology Reference

    Type
    Guide
    ... for an empty plasmid backbone for your experiment, see Addgene's empty backbone page for more information...of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can...unique and not located elsewhere in the plasmid backbone, which is why they can be used for cloning by ...Antibiotic Resistance Choosing Your Perfect Plasmid Backbone 6 tips for analyzing and troubleshooting sanger...

  4. Modular Cloning Guide

    Type
    Guide
    ...terminators, 4 nonfunctional "dummy" parts, 34 backbones, and 6 destination vectors for use in E. coli ...including CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for genome editing in monocots ...expression Christopher Dupont 95 plasmids including backbones, fluorescent proteins, protein tags, and signal...

  5. CRISPR Guide

    Type
    Guide
    ...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading and discrimination...Schnitzbauer, J., Zhang, W., Li, G., Park, J., Blackburn, E. H., Weissman, J. S., Qi, L. S., & Huang, B...

  6. Science Guides

    Type
    Guide
    ... Plasmids within a given library have the same backbone, but they express or target different genes. Some...

  7. Guide to Using Pooled Libraries

    Type
    Guide
    ... Plasmids within a given library have the same backbone, but they express or target different genes. Some...

  8. Sequencing Primers

    Type
    Guide
    ...repository, we've chosen primers based on the plasmid backbone and insert. To identify primers that may be useful...
Showing: 1 - 8 of 8 results