We narrowed to 74 results for: flag

1. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...offers C- and N-terminal tagging vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin or ...

2. AAV Vector Quality Control: Going the Extra Mile with NGS

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   ...threshold at >100 hits - immediately raises a red flag. For example, AAV-68544 contained 220 hits to the...

3. PITChing MMEJ as an Alternative Route for Gene Editing

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   ...Zhang et al. shows just that - using MMEJ to insert FLAG tags into the genome of the pathogenic fungus Aspergillus...

4. Neuronal labeling with Spaghetti Monster

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   ...virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-tag and...lacunosum moleculare were better resolved with smFP_FLAG than GFP.   3. Enhanced ability to distinguish...

5. Isolating Genomic Regions of Interest with the CRISPR System

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   ...et al. "Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells...cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting their genomic... In addition to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have...Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9...chromatin or DNA fragment (Fujita et al., 2016). 3xFLAG-tag fused to dCas9, along with a biotinylated gRNA...

6. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   ...Expression system Stable cell line expressing 1) FLAG-biotin-tagged dCas9, 2) BirA, and 3) one or more...

7. Tips for arabidopsis transformation

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   ...(YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also a variety of plant selection markers...

8. QC Sequencing Technologies at Addgene

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   ...identity and plasmid length. Any mismatch or indel flags the sequence for manual review by one of our QC ...

9. Genetic Code Expansion

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   ...TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84 tRNA synthetase M. barkeri...154764 pAS_FLAG-Mx1201 PylRS PylRS M. alvus Mammalian TAG Simon Elsaesser 154768 pAS_4xG1 PylT FLAG-G1 PylRS...140018 pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG tyrosyl-tRNA synthetase E. coli Mammalian TAG Simon Elsaesser 140019...Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian... Simon Elsaesser 154773 pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian...TAG Simon Elsaesser 154774 pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS PylRS M. mazei Mammalian TAA Simon Elsaesser...4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG Simon Elsaesser 140012 pAS_4xMx PylT_FLAG-Mx PylRS...

10. CRISPR Plasmids - Yeast

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    ...The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence...

11. CRISPR Plasmids - Bacteria

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    ...The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence...Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation...

12. CRISPR Plasmids - Mammalian Expression

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    ...The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system...

13. TALEN Expression Vectors

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    ... TALENs expressed from these vectors also have a FLAG epitope tag and nuclear localization signal at their...

14. Antibodies 101: Antibody Engineering and Directed Evolution

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    ...more coming soon) as well as epitope tags like His, Flag, and Biotin. The antibodies developed by IPI are...

15. Ginkgo Bioworks COVID-19 Collection

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    ...bacterial, yeast, or mammalian) Tags (e.g. 6xHis, Flag, StrepII, HA, Myc, SUMO, GST, MBP, CBP, S-tag, TAP...

16. Structural Genomics Consortium Plasmids

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    ...KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106 pNH-TrxT GU269914...

17. How to Deposit Your Plasmids with Addgene

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    .... Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use the drop-down menu...

18. CRISPR References and Information

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    ... 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA...pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional...

19. Antibody Validation for Flow Cytometry

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    ...expression measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate...

20. 27 Hot Plasmids from 2016

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    ...generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient experimental use. The plasmids...summarized in the table below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid...