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We narrowed to 73 results for: flag

Showing: 21 - 40 of 73 results

  4. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...et al. "Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells...cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting their genomic... In addition to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have...Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9...chromatin or DNA fragment (Fujita et al., 2016). 3xFLAG-tag fused to dCas9, along with a biotinylated gRNA...

  5. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-tag and...lacunosum moleculare were better resolved with smFP_FLAG than GFP.   3. Enhanced ability to distinguish...

  8. Genetic Code Expansion

    Type
    Collection
    ...TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84 tRNA synthetase M. barkeri...154764 pAS_FLAG-Mx1201 PylRS PylRS M. alvus Mammalian TAG Simon Elsaesser 154768 pAS_4xG1 PylT FLAG-G1 PylRS...140018 pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG tyrosyl-tRNA synthetase E. coli Mammalian TAG Simon Elsaesser 140019...Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian... Simon Elsaesser 154773 pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A M. archaeon Mammalian...TAG Simon Elsaesser 154774 pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS PylRS M. mazei Mammalian TAA Simon Elsaesser...4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG Simon Elsaesser 140012 pAS_4xMx PylT_FLAG-Mx PylRS...

  9. CRISPR Plasmids - Yeast

    Type
    Collection
    ...The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence...

  10. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence...Insert Promoter PI Publication 64325 3xFLAG-dCas9/p-bacteria 3xFLAG-dCas9 pLtetO-1 Fujii Efficient isolation...

  12. TALEN Expression Vectors

    Type
    Collection
    ... TALENs expressed from these vectors also have a FLAG epitope tag and nuclear localization signal at their...

  17. CRISPR References and Information

    Type
    Collection
    ... 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA...pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient experimental use. The plasmids...summarized in the table below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid...

  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ...amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible...
Showing: 21 - 40 of 73 results