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We narrowed to 74 results for: flag

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Showing: 21 - 40 of 74 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for ...fusing your protein to an epitope tag, such as Flag or HA, will allow you to easily identify your...expression pNIC-CTHF - C-terminal TEV-His6-Flag for bacterial expression pFA6a vectors - PCR-based... expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression... SV40 NLS Myr Membrane localization pWZL-Neo-Myr-Flag-DEST - pDest vector that adds an ... tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG tag in pCS for a variety of...

  7. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-tag and...lacunosum moleculare were better resolved with smFP_FLAG than GFP.   3. Enhanced ability to distinguish...

  8. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...et al. "Transgenic mouse lines expressing the 3x FLAG‐dC as9 protein for enCh IP analysis." Genes to cells...cells by expressing dCas9 with an N-terminal 3xFLAG tag (3xFLAG-dCas9) and a gRNA targeting their genomic... In addition to 3xFLAG and biotin tags, use of tag systems including Protein A, 1xFLAG, 2xAM, and HA have...Immunoprecipitation, using an antibody targeting the 3xFLAG tag, allowed these researchers to isolate dCas9...chromatin or DNA fragment (Fujita et al., 2016). 3xFLAG-tag fused to dCas9, along with a biotinylated gRNA...

  10. Neurodegeneration Research Collection

    Type
    Collection
    ...the 3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with GFP, or IP with Flag tag...et al. J Biol Chem. 2006) Express human ATM with Flag and His tags. (Canman et al. Science. 1998) See ...

  11. Genetic Code Expansion

    Type
    Collection
    ...TAG Ryan Mehl 174081 pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0 NES-Flag-R2-84 tRNA synthetase M. barkeri...154764 pAS_FLAG-Mx1201 PylRS PylRS M. alvus Mammalian TAG Simon Elsaesser 154768 pAS_4xG1 PylT FLAG-G1 PylRS...140018 pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG tyrosyl-tRNA synthetase E. coli Mammalian TAG Simon Elsaesser 140019...Mammalian TAG Simon Elsaesser 154769 pAS_4xG1 PylT FLAG-G1 PylRS Y125A G1 PylRS Y125A Methanogenic archaeon... Simon Elsaesser 154773 pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A G1 PylRS Y125A Methanogenic archaeon...TAG Simon Elsaesser 154774 pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS PylRS M. mazei Mammalian TAA Simon Elsaesser...4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG Simon Elsaesser 140012 pAS_4xMx PylT_FLAG-Mx PylRS...

  16. CRISPR References and Information

    Type
    Collection
    ... 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA...pCRISPR PDF, 109 KB Mendenhall and Myers Mammalian: FLAG tagging endogenous proteins pFETCh_Donor ; additional...

  18. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...generation lentiviral transfer vector. Each gene has been FLAG-tagged for convenient experimental use. The plasmids...summarized in the table below:   ID Cv (Capsid) FLAG Tag Terminus 79629 (N) and 79628 (C) Cv (capsid...

  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...

  20. Zebrafish Plasmid Collection

    Type
    Collection
    ...amino- or carboxyl-terminal tags, including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible...
Showing: 21 - 40 of 74 results