We narrowed to 102 results for: fluorophore

... curve with known amounts of protein. Fluorophores Fluorophores are light-sensitive molecules which emit...IF uses fluorophore color to differentiate between the targets of interest, if your fluorophore is bound...the fluorophores used is important. It is best to inversely pair the brightness of the fluorophore with...If a bright fluorophore is on an abundant target, it obscures fainter signals. Fluorophores can emit signals...storing your antibody: Antibodies conjugated to fluorophores should never be stored below 4 °C. Certain isotype...molecules are horseradish peroxidase (HRP) and fluorophores. Horseradish peroxidase (HRP) HRP performs a...specific wavelength. While more expensive than HRP, fluorophores are available in a range of colors activated...

...you image the first target, you inactivate the fluorophores in the sample, either by chemical treatment ...and that each secondary has a different color fluorophore that you can image separately on your microscope...labeling sequentially with one, inactivating the fluorophores, then labeling with the other, you can still...using primary antibodies that are conjugated to fluorophores. However, the primary-secondary workflow (a....fluorescently-conjugated streptavidin to get more fluorophores per antibody, or conjugate with an enzymatic...

...these molecular tools. Cre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI....none 8 James M. Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s) PI 50363 AAV phSyn1(S)-DreO-bGHpA..., rg* Hongkui Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA...Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none...PHPeB Hongkui Zeng VCre AAV ID Name Promoter Fluorophore/Tag Serotype(s) PI 55638 pAAV-EF1a-vCre EF1a ...

...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

...various teal, green, yellow, orange, and red fluorophores. The spectra for mOrange2 (scaffold of OCaMP...at 565 nm fits neatly between yellow and red fluorophores, allowing it to be paired with green, yellow...non-tunable, their usefulness depends on selecting a fluorophore matched to their excitation wavelength. OCaMP...

...of energy from an excited donor fluorophore to an acceptor fluorophore, which then emits it as light. ...transferred from a luminescence reaction to a nearby fluorophore, which emits light. No excitation illumination...

...strategies. The green structure represents a fluorophore or other useful ligand, gray structure is the...non-natural amino acids to directly incorporate a fluorophore in your protein or to incorporate an alkene to...years; improving the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability...Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores...better-dyeing-through-chemistry-small-molecule-fluorophores  Los, G. V., Encell, L. P., McDougall, M. G....

...their technology Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP (yellow), and membrane-tethered..., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the...clearing methods and can be labeled by synthetic fluorophores, which are more stable than fluorescent proteins...see systems that use newer, more photostable fluorophores and that can be combined with new imaging techniques...

...non-radiatively transferred from an excited donor fluorophore to an acceptor. Because this process is extremely...extremely sensitive to the distance between the fluorophores at the single-nanometer scale, scientists can...lifetime (the time between excitation of the fluorophore and emission of a photon) of the donor can be...type, relative position and orientation of the fluorophores through linkers, fluorescent protein expression... and biosensors can also incorporate organic fluorophores via HaloTag or SNAP-Tag . Read more about self-labeling...

...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

... partner, which directed localization of the fluorophore, and thus enabled labeling of particular structures...with strong expression and detection of each fluorophore at the respective subcellular structure (Figure...for Labeling Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner ...

... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in .... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...localization. These could be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence...unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from...

...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...

...fluorescent protein joins mTFP1 and mWasabi, as fluorophores from Allele Biotechnology that now can be deposited...useful in multicolor labeling particularly with fluorophores that excite in the violet range. For example...

...FRET), in which a donor fluorophore is used to excite an acceptor fluorophore. The difference for BRET...

...signal. Prior to the development of these probes, fluorophore binding aptamers and MS2-binding fluorescent ...aptamers or MS2 tags using the same number of fluorophores. You can find plasmids containing the cobalamin...

...use a red fluorophore-tagged anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse...

...should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

...high brightness is an important feature of a fluorophore. The key question is how the brightness is best...Theoretical brightness Two key properties of fluorophores that determine brightness are the extent to ...

...light source, a cell expressing the associated fluorophore or stained with the associated fluorochrome, ...fluorescence. A cell expressing high levels of the fluorophore or fluorochrome-stained protein will emit a stronger...expression, measured using antibody conjugated fluorophores Fluorescent antibodies get around the challenges...