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We narrowed to 99 results for: fluorophore

Showing: 21 - 40 of 99 results

  1. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore (circularly...relies on the fluorescence ratio between the 2 fluorophores, rather than on the fluorescence intensity, ...

  2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...caused partially by fluorophore photoinstability, as well as the tendency of fluorophores to aggregate in ...permit analysis by immunostaining. Although the fluorophores used are fluorescently distinct, their protein...preventing design of antibodies specific to each fluorophore. Third, Brainbow-1 and Brainbow-2 each contained...3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the default state. In contrast,...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...you image the first target, you inactivate the fluorophores in the sample, either by chemical treatment ...and that each secondary has a different color fluorophore that you can image separately on your microscope...labeling sequentially with one, inactivating the fluorophores, then labeling with the other, you can still...using primary antibodies that are conjugated to fluorophores. However, the primary-secondary workflow (a....fluorescently-conjugated streptavidin to get more fluorophores per antibody, or conjugate with an enzymatic...

  4. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...conjugated to a detection substrate, such as a fluorophore, or a conjugated secondary antibody that will...of primary antibodies conjugated to different fluorophores or a panel of primaries that was raised in different...isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific to each...

  5. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...strategies. The green structure represents a fluorophore or other useful ligand, gray structure is the...non-natural amino acids to directly incorporate a fluorophore in your protein or to incorporate an alkene to...years; improving the labeling kinetics or the fluorophore’s microenvironment to enhance brightness, stability...Better Dyeing Through Chemistry & Small Molecule Fluorophores. Addgene Blog. https://blog.addgene.org/better-dyeing-through-chemistry-small-molecule-fluorophores...better-dyeing-through-chemistry-small-molecule-fluorophores Los, G. V., Encell, L. P., McDougall, M. G.,...

  6. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...their technology Brainbow. Brainbow 1.0 used the fluorophores RFP (red), YFP (yellow), and membrane-tethered..., 2013), incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the...clearing methods and can be labeled by synthetic fluorophores, which are more stable than fluorescent proteins...see systems that use newer, more photostable fluorophores and that can be combined with new imaging techniques...

  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...of course—and start tagging! Yeast-optimized fluorophores for imaging Lee S, et al. (2013) PLoS ...requirements. Select from these yeast-optimized fluorophore tagging vectors for your single or multi-color...

  8. MXS Chaining

    Type
    Blog Post
    ... partner, which directed localization of the fluorophore, and thus enabled labeling of particular structures...with strong expression and detection of each fluorophore at the respective subcellular structure (Figure...for Labeling Cellular Structures Construct Fluorophore Excitation/emission maxima Tethering partner ...

  9. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ... primary antibodies that are conjugated to a fluorophore, to avoid the use of secondary antibodies in .... Because the signal comes from the antibody-fluorophore conjugate, and is not expressed by the cell itself...localization. These could be visualized using fluorophore-conjugated antibodies (i.e., immunofluorescence...unconjugated primary antibody, then visualized using a fluorophore-conjugated secondary antibody or Fab. Image from...

  15. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...should have a single color control for every fluorophore you intend to use in your panel. Using single...have the same Ig subclass, and be in the same fluorophore (e.g., if you are staining for anti-mouse Ly6C...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ... tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Conformationally... sensor created by fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Nano-lanterns...-Leu linker to the circularly permuted Venus fluorophore. Learn more about pHlash sensor in our 28 Hot...

  18. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...light source, a cell expressing the associated fluorophore or stained with the associated fluorochrome, ...fluorescence. A cell expressing high levels of the fluorophore or fluorochrome-stained protein will emit a stronger...expression, measured using antibody conjugated fluorophores Fluorescent antibodies get around the challenges...

  19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...been introduced to GFP and new iterations of fluorophores are constantly being engineered. Table 1 lists... 1: The specific mutations comprising common fluorophores Fluorescent Protein Mutations Relative to...

  20. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... This process can be avoided direct antibody fluorophore conjugates are used, but these are usually more...secondary nanobodies conjugated with the desired fluorophores. In figure 4B below, triple colocalization is...
Showing: 21 - 40 of 99 results