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Showing: 21 - 29 of 29 results

  1. TALEN Plasmids and Kits

    Type
    Collection
    ...Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly...ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru/Cowan Lab TALEN Kit... for deletion mutagenesis and gene targeting in human and mouse pluripotent stem cells. 48706 pTAL7b Joung... TALE repeats (C, T, and G) and the full length human LSD1 at the carboxy-terminus of the protein. 49043...in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids...rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...

  2. Genetic Code Expansion

    Type
    Collection
    ...pCMV-MtPylRS (human-opti) PylRS M. thermophila acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS (human-opti)...pCMV-MtAcKRS (human-opti) AcKRS M. thermophila acetyl-lysine Mammalian Tao Liu 164196 pCMV-MfBulKRS (human-opti...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...

  3. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...grandparents' slow growth period." European Journal of Human Genetics 10.11 (2002): 682-688. PubMed PMID:12404098...reporter to target the K9 (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27 histone methylation...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Aebischer 11442 pBabe bleo human Frataxin FXN Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag)...11503 pBabe neo human Frataxin FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich...140575 CaV2.1/pcDNA3.1 ~ Human CACNA1A CMV, T7 Spinocerebellar ataxia 6 Dan Minor 141189 pcDNA4/TO/GFP-GIGYF2... Friedreich ataxia Ronald Kahn 11505 pBabe puro human Frataxin FXN Friedreich ataxia Ronald Kahn 11508...ApoEpromoter APOE ApoE Alzheimer's Sohail Tavazoie 51437 human Alpha-synuclein SNCA T7 Parkinson's Michael J Fox...-Myc-B HTT Myc UbC Huntington's Baoji Xu 110758 Human Parkin (1-465) PRKN His, GST T7 Parkinson's David...CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary diffuse leukoencephalopathy...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https:...mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core, as well as pLV-dCas9... in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a ...

  7. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin GFP Adherens Junctions Beta-catenin...Autophagosome LC3 EGFP Tamotsu Yoshimori 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ... CIneo-RL Renilla CMV Mammalian expression of humanized renilla luciferase Witold Filipowicz 100984 pGL4.18...Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla... processes, biosensors, or optogenetic tools . pcDNA3.1+-pHlash , pRSETb-pHlash : A pH sensor created ...Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc® Creation of N-terminal Nanoluc...Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation of N-terminal Nanoluc...fusions using Gateway cloning Mikko Taipale 87065 pcDNA3.1-ccdB-Renilla Renilla Creation of C-terminal Renilla...fusions using Gateway cloning Mikko Taipale 87066 pcDNA3.1-ccdB-Firefly Firefly Creation of C-terminal Firefly...

  9. Cre-lox system

    Type
    Collection
    ...the human Estrogen Binding Domain (EBD) TDH3 Yeast van Leeuwen 64770 pSS146 Cre fused to the human Estrogen...122960 pcDNA3.1_PA-Cre photo-activatable Cre recombinase CMV Mammalian Sato 122961 pcDNA3.1_PA-Cre-Y324F...Binding Domain (EBD) Tdh4 Yeast van Leeuwen 65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular...
Showing: 21 - 29 of 29 results