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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31 results for: human cdnas

Showing: 21 - 31 of 31 results

  1. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...RNA is reverse transcribed into the provirus, the cDNA that will be integrated into a host genome. This...-retroviral vectors can be packaged directly in human embryonic kidney 293T (HEK293T) cells (Figure 3A.... M. (2003). Transcription start regions in the human genome are favored targets for MLV integration. ...leading to disease. Provirus The genetic material (cDNA for retroviruses) that will be integrated into a...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... both human and fly versions. Arnold, et al. Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral...   Human kinase domain constructs for automated bacterial expression The misregulation of human kinases...Lab recently compiled the Human Kinase Domain Constructs Kit, a library of human kinase domain constructs...gRNAs targeting a total of 20,121 genes within the human genome (the identity of the genes and target sequences...repository that can be used to perform screens in human cells. General information regarding how to use ...receptors (GPCRs) yet one third of the GPCRs in the human genome are orphan receptors meaning their ligands...allows researchers to interrogate the druggable human GPCR-ome using a quick, affordable, and ubiquitous...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...species. Some common types of lentivirus include Human immunodeficiency virus (HIV), bovine immunodeficiency...RNA is reverse transcribed into the provirus, the cDNA that will be integrated into a host genome. This...process requires a production cell line, typically human embryonic kidney 293T (HEK293T) cells. Standard ...2013). Biosafety features of lentiviral vectors. Human Gene Therapy , 24 (2), 132–142. https://doi.org/... Genome-Scale CRISPR-CAS9 knockout screening in human cells. Science , 343 (6166), 84–87. https://doi....serious diseases. Provirus The genetic material (cDNA for retroviruses) that will be integrated into a...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ...Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang LIC TAL Effector Assembly...ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru/Cowan Lab TALEN Kit... for deletion mutagenesis and gene targeting in human and mouse pluripotent stem cells. 48706 pTAL7b Joung... TALE repeats (C, T, and G) and the full length human LSD1 at the carboxy-terminus of the protein. 49043...in labeling specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids...rate of Golden Gate assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression...in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination...

  5. Genetic Code Expansion

    Type
    Collection
    ...pCMV-MtPylRS (human-opti) PylRS M. thermophila acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS (human-opti)...pCMV-MtAcKRS (human-opti) AcKRS M. thermophila acetyl-lysine Mammalian Tao Liu 164196 pCMV-MfBulKRS (human-opti...L-lysine (TFA-Lys) Bacterial TAG Thomas Huber 182287 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF) Y306A/Y384F (AF...PylRS M. mazei Bacterial TAA Wenshe Liu 182653 pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...grandparents' slow growth period." European Journal of Human Genetics 10.11 (2002): 682-688. PubMed PMID:12404098...reporter to target the K9 (pcDNA3-K9 histone methylation reporter) and K27 (pcDNA3-K27 histone methylation...

  8. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin GFP Adherens Junctions Beta-catenin...Autophagosome LC3 EGFP Tamotsu Yoshimori 24920 pEGFP-LC3 (human) Autophagosome LC3 EGFP Toren Finkel 14435 mRFP-...Extracellular milieu cfSGFP2-N EGFP Ikuo Wada 73208 pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3 Mitochondria COX IV-derived...Nuc Nucleus NLS mKalama1 Robert Campbell 73205 pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3 Nucleus NLS (from Mak16p...protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3 Cytosol, nucleoplasm None...sequence from p63 mTurquoise2 Dorus Gadella 73206 pcDNA3.1-kappa-myc-dL5-2xG4S-TMst Cell surface (mammalian... Peroxisome SKL mNeonGreen Dorus Gadella 73210 pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL Peroxisome SKL ...

  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https:...mammalian expression constructs including pcDNA-dCas9-p300 Core and pcDNA3.3-Nm-dCas9-p300 Core, as well as pLV-dCas9... in mammalian cells. This plasmid is used with pcDNA3.1-MS2-Tet1-CD to decrease methylation and activate...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a ...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Aebischer 11442 pBabe bleo human Frataxin FXN Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag)...11503 pBabe neo human Frataxin FXN Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich...140575 CaV2.1/pcDNA3.1 ~ Human CACNA1A CMV, T7 Spinocerebellar ataxia 6 Dan Minor 141189 pcDNA4/TO/GFP-GIGYF2... Friedreich ataxia Ronald Kahn 11505 pBabe puro human Frataxin FXN Friedreich ataxia Ronald Kahn 11508...ApoEpromoter APOE ApoE Alzheimer's Sohail Tavazoie 51437 human Alpha-synuclein SNCA T7 Parkinson's Michael J Fox...-Myc-B HTT Myc UbC Huntington's Baoji Xu 110758 Human Parkin (1-465) PRKN His, GST T7 Parkinson's David...CHMP2B mCherry CMV ALS Sanford Simon 115523 MSCV-human CSF-1Rwt-IRES-GFP CSF1R Hereditary diffuse leukoencephalopathy...

  11. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...expression atlas of miRNAs and their promoters in human and mouse. Nature Biotechnology, 35(9), 872–878....between the red boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence...
Showing: 21 - 31 of 31 results