...investigators realize too late that they have missed the impact of dark, non-fluorescent, and misfolded FP-fusions...quantitative imaging experiments or even negatively impact cells (see Figure 1). When behaving properly, ... the function of resident ER chaperones and may impact cellular function or possibly viability. FP misfolding.... It remains unclear whether the FP misfolding impacts fusion protein function and this could lead to ...

...Discrepancies in transduction efficiency can have a major impact on experimental design and interpretation of results...transduced by the vector. One method for minimizing the impact of this phenomenon is to use a retrograde recombinase-expressing...cell bodies of the projection neurons reside.   Impact for scientists While the mechanisms by which retrograde...

... resulting in variable gene expression that can impact observed phenotypes. Factors such as the growth...medium, growth temperature, and growth rate can all impact plasmid copy number in a cell. One can attempt... from a plasmid to the genome without greatly impacting the level of gene expression. For example, the...

...experiment. Here, we’ll discuss NHEJ, and how it impacts what happens to Cas9-mediated DSBs in the genome...chromosomes within a day. Another factor expected to impact repair is that the Cas9 protein doesn’t immediately...in determining how the structure of broken ends impacts how they are repaired. He works in Dale’s lab.  ...

...long-term science outreach, may have the most profound impact (15). Diversity and inclusion in science, technology... immigrants, and minorities who have had great impacts on STEM (21–26). Increased scientific funding...communities (something already encouraged by “Broader Impacts” requirements for certain grants) (28). As one ...Science Foundation 15-008: Perspectives on Broader Impacts. The Pipettepen: The March for Science Raises Concerns...

...plasmid. The requirement for additional DNA can impact the transfection efficiency of the packaging cell... and the timing and number of harvests will all impact the titer. Importantly, optimized conditions for...virus, volume and timing of transduction will all impact the recorded titer and should be taken into consideration...

... differences, and plasmid recombination can all impact the representation of individual plasmids in the...

...TCCGGGATCCGGGCTGCGgctgctgttcctcctgCGCCCTGCGGAGCGGCGA Impact homolog (mouse) TAL3462 & TAL3463 TGGACAAATTAGAGGATCatgacgataataatctGCAGTCTCAGATAGAGGA...

... over-reliance on the perfect story for a “high impact” publication can lead to the publication of rare... data even if it isn’t considered immediately impactful. Down the line we may see further adoption of ...criticisms that focus less on perceived novelty and impact. Journals could also begin requiring reproducibility...health of the scientific enterprise and not just impact. Cause #3: Poor experimental design It’s easy to...

...are sharing stories of our repository’s positive impact on science and scientists around the globe. Our...CRISPR plasmids to labs in ~100 countries.  The impact Addgene’s CRISPR collection contained 197 plasmids...citations.  CRISPR is a story that centers around the impact of science, but our data makes it clear that it...

...of the individual’s work in their field and the impact of the individual’s past work on the field. NIW...experts in the field. You must also be able show the impact of your influence on your field of employment. ...contributions to the field that have had a major impact. For example, you were the first person to identify...that you are given to present your expertise and impact on your field from the point of view of experts...current research goal is and why your work has impacted your field (i.e. finding vaccines for deadly disease...

...these milestones remind us of the extraordinary impact that happens when scientists come together to share...breakthrough enabled brings us closer to making an impact on humanity's greatest challenges. Thank you for...

...role in fighting the pandemic could be even more impactful by reaching more scientists. Many members of the...past months, we’re excited to see our open sharing impact grow beyond COVID-19. But, we can’t do this alone...

...variety of metrics they can use to evaluate the impact of their work (H-index for instance), but these...the Addgene Blog Read How Notions of Scientific Impact Are Evolving Check out the Blog Post on Our Citations...

...circulating tumor cells (CTCs) is going to have a big impact on our understanding and treatment of cancer. It... I hope that our antibody tentacles may have an impact on this challenge either for magnetic capture or...

...engage their readers or listeners thus expanding the impact and appreciation for their research. Long story...judgement. Eventually, we published the paper in a high-impact journal and it worked out for all of us.” This ...

...to revisit some of the ways these animals have impacted humans through biomedical research. The Camelid...And I suspect we will only continue to see their impact grow as new sdAbs become easier to generate and...

..., and disease. In order to expand their model’s impact, organoid users have recently begun to develop ...these novel biosensors could have a major economic impact.   Design and mechanism of action of the...

...time. “At an early age, I was fascinated by the impact that medicines can have on human health. As a teenager...organizations are less common, they can be very impactful. At Novartis, Stegmeier deposited the ClonTracer...

... increasing the impact of the work, and as a collateral benefit, increasing the impact of the specific...