We narrowed to 32 results for: ires-cre/1000

...primers to create 200-300 bp sequencing products using PCR. A diagram of the product we created for the ...you want to have 100x-1000x coverage of your library: in other words, 100-1000x more reads than number...plasmid. However, lots of library DNA is needed for screening experiments, so the scale of the DNA prep is larger...rank the plasmids by abundance, scaled to 1, to create a “cumulative wealth distribution” curve, or Lorenz...a powerful research tool, but their proper use requires careful amplification and maintenance of good ...wishes you luck in your future library use and screening, and if you have any tips, input, or stories of...

...Systemic capsids packaging Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse...with appropriate cell type-specific promoters or Cre lines. Spatial transcriptomics suggests that AAV-...Huber, N., Pasca, S. P., & Gradinaru, V. (2016). Cre-dependent selection yields AAV variants for widespread...Deverman, B. E., & Gradinaru, V. (2020). Multiplexed Cre-dependent selection yields systemic AAVs for targeting...have developed techniques like CREATE (Deverman et al., 2016), M-CREATE (Ravindra Kumar et al., 2020),...Research in Neurobiology, 4, 100086. https://doi.org/10.1016/j.crneur.2023.100086. Challis, R. C., Ravindra...

...and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 O’Brien, R. E., Bravo, J...part of the Class 1 CRISPR family, which means it requires multiple Cas effectors to carry out genomic editing...different compositions of the Cascade complex. Created with BioRender.com.   The Cascade complex Cascade...Figure 2: Cascade complex from CRISPR type I-E. Created with BioRender.com. Thanks to this complex,...Tomoji Mashimo lab and the Tilmann Weber lab — to increase efficiency of these systems, though consistent...General overview of the Cascade-Cas3 mechanism. Created with BioRender.com.   The reeling mechanism...unwinding of dsDNA occur simultaneously, which creates a loop in the target strand that is cut occasionally...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette Ice bucket CO 2 incubator...likelihood of multiple integration events decreases as the dilution increases. Calculate the transduction units...cell lines and selection markers. Note this assay requires staining resistant colonies with crystal violet... remove cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing...

... Zinc Finger Consortium: OPEN Reagents (Kit # 1000000013 ) Depositing Labs: Keith Joung This kit consists...17. PubMed . Description Use of the OPEN method requires OPEN pools (available by request from the Joung...paper . How to Cite this Kit These plasmids were created by your colleagues. Please acknowledge the Principal...Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods...publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your Reference section: Oligomerized pool...

...useful tool for marking and tracking cells is the the Cre/lox recombination system. A researcher can flank ...expression is turned on or off in cells expressing Cre. The result of a system like this is a mosaic of ...respectively. The permanent labelling of cells using cre-lox is advantageous when compared to photoactivable... In the past 15 years efforts have been made to create transgenic mice expressing fluorescent protein ...auto-fluorescence (Drobizhev et al. 2011). But this requires other lasers than the standard Ti:Sapphire laser...wavelength as it has been shown that even a small incremental change of the excitation wavelength can significantly...called Brainbow, the Ubow mouse strain has been created to fate map Langerhans cells and follicular dendritic...

...breaks caused by Cas12 create short 3’ overhangs, which leads to slightly increased efficiency when conducting...and Genome Editing, 3–4, 100013. https://doi.org/10.1016/j.ggedit.2022.100013 Additional resources on ...systems has led to extreme sequence divergence that requires a multi-pronged analysis to sort effectively. ...type to subtype to variant (shown in italics). Created with BioRender.com.   Class 1 versus class ...underutilized compared to Class 2. Class 1 Despite their incredible abundance, Class 1 CRISPR systems are less popular...Cascade. As you might imagine, this is handy for creating large genomic deletions. Type I systems are also...with Cas3 (pink). Complex shown is from type I-E. Created with BioRender.com.   Type III Type III is ...

...used for large scale edits. These proteins, like Cre recombinase or phage derived serine integrases, insert...GAT; increased nuclease fidelity SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease...others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...backbone HypaCas9 — increase Cas9 proofreading and discrimination evoCas9 — decrease off-target effects...domains to expand PAM recognition, increase specificity, and decrease off-target effects Sniper-Cas9 — ...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 — increases fidelity with reduced nuclease...integration of donor DNA. Genome-Wide Screens Using CRISPR In a genetic screen, a population of cells with mutations...

... ligase The Roux Lab in 2016 optimized BioID to create BioID2, a smaller and improved promiscuous biotin..., BioID2 exhibits enhanced proximal labelling, requires less biotin, and allows scientists to more selectively...was its low sensitivity. Sensitivity could be increased with higher expression levels but for many fusion...until they identified a mutant with significantly increased sensitivity that did not affect the function or...miniTurbo were developed in the Ting Lab in 2018 to increase the speed of proximity labeling by promiscuous...APEX2, although it can rapidly label proteins, requires H2O2 which can be toxic to living cells. To circumvent... BASU exhibits 1,000 fold faster kinetics and increases the signal to noise ratio 30-fold compared to ...

...channel pipette 20–200 µL single channel pipette 200–1000 µL single channel pipette 1–10 µL multichannel pipette...enough master mix for all samples). Each sample requires 15 μL of master mix. Pro-Tips Use a "Universal...95 uL 20X 200X Dilution 3 20 uL Dil. 2 80 uL 5X 1000X Dilution 4 20 uL Dil. 3 80 uL 5X 5000X Dilution ...indicates the presence of primer dimers which can increase background signal and alter the Ct values of your...

...reactions. The BP reaction creates an attL-flanked entry clone. The LR reaction creates an expression clone ...entry clone and destination vector creates two products: the desires expression clone and a byproduct containing...reactions. Recombination of attP and attB sites creates attL and attR sites.   Gateway vectors contain...Gateway plasmids.    Figure 3: Method A to create an entry clone: recombination of an attB-flanked...attL-containing entry vector.   Figure 4: Method B to create an entry clone: TOPO cloning the insert into an...attL-containing entry vector.   Figure 5: Method C to create an entry clone: Restriction cloning the inset into...vector will recombine with the attL-entry clone to create the expression clone.    Figure 6: Generating...

...used for large scale edits. These proteins, like Cre recombinase or phage derived serine integrases, insert...GAT; increased nuclease fidelity SpCas9-NG — NG; increased in vitro activity SpG — NGN; increased nuclease...others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...backbone HypaCas9 — increase Cas9 proofreading and discrimination evoCas9 — decrease off-target effects...domains to expand PAM recognition, increase specificity, and decrease off-target effects Sniper-Cas9 — ...compatible with truncated gRNAs to increase specificity SuperFi-Cas9 — increases fidelity with reduced nuclease...integration of donor DNA. Genome-Wide Screens Using CRISPR In a genetic screen, a population of cells with mutations...