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We narrowed to 187 results for: lentiviral

Showing: 21 - 40 of 187 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway...for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing and nondividing ...integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression...

  2. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ... Service Suggestion Form. Lentivirus As some of the most popular lentiviral vectors in the repository ...collection. We’ll provide a brief rundown of what lentiviral and AAV preps are available below, but you can... tools, we’re starting the Viral Service with lentiviral preps for the delivery of Cas9 endonuclease, ...with viruses including advice on how to titer lentiviral preps, troubleshoot viral transductions, and ...Virus in Your Research: A Primer for Beginners Lentiviral Vector Uses and Overview AAV: A Versatile Tool... of our new Viral Service, we’re distributing lentivirus (with many CRISPR tools included among the preps...we’ve got viruses to help you out. Of course, lentiviruses are used in experiments other than just those...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...interest Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...Firefly Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 212935 pGL4.84(hRlucCP/Puro) RapidResponse...Renilla Insertion of 5' promoter/enhancer regions. Lentiviral Nadav Ahituv 108228 pGreen2x35SLUC Firefly, Renilla...21471 pLenti PGK V5-LUC Neo (w623-2) Firefly PGK Lentiviral expression of firefly luciferase Eric Campeau... pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase Eric Campeau... 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral expression of firefly luciferase Christopher ...

  4. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...examples of each in Addgene's collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles...Plasmids Envelope and Packaging Plasmids Popular Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus...neutralizing activity are listed below: pBOBI-FLuc - Lentiviral reporter vector expressing firefly luciferase...firefly luciferase. pHAGE-CMV-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase...luciferase and ZsGreen. NL4-3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly... lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee...

  5. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

    Type
    Blog Post
    ... those are lentiviral constructs. In 2007 we continued to see requests grow for lentiviral packaging and... pLKO.1 puro and pLKO.1 – TRC cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO ...-puro for retroviral expression and pLVTHM, a lentiviral plasmid for mammalian gene silencing. TALENs ...

  6. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    ... Viral vectors are prone to recombination In lentiviral vectors, recombination often occurs between the...full length plasmid.   Figure 1: The full lentiviral plasmid is expected to be >12 kb. However if ...noticed that in Stbl3 bacterial cultures with lentiviral vectors, the flat white colonies contained the... Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech... (2007) Improved recombinational stability of lentiviral expression vectors using reduced-genomeEscherichia...

  7. Kazuhiro Oka Lentiviral Vectors

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    Collection
    ...deposited a series of lentiviral and adenoviral transfer vectors with Addgene. Lentiviral Transfer Vectors ...For more information on lentiviral and adenoviral vectors read our Lentiviral Vector Guide and Adenoviral... in our Lentiviral Plasmids and Adenoviral Plasmids collections Addgene Blog: Your Lentiviral Plasmid ...vectors. Download the Lentivirus Transfer Vector Production Protocol Lentiviral Vector ID Purpose pCDH-EF1...Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core Depositor... Viral Vectors Gene Vector Core Viral Vectors Lentiviral Transfer Vectors Adenoviral Transfer Vectors ...Vectors Within this set of lentiviral transfer vectors you can find reporter plasmids to test whether or not...

  8. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...use lentivirus to deliver a pooled population of gRNAs to Cas9 expressing cells. Pooled lentiviral CRISPR...a heterogeneous population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene...synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library...amplification. If you've obtained a ready-to-use lentiviral preparation from Addgene, you can skip the steps...publication “Practical Considerations for Using Pooled Lentiviral CRISPR Libraries” (McDade et al., 2016). To learn...2016) Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular...generate lentivirus (B). Cas9-expressing cells or wild-type cells are treated with lentivirus containing...

  9. Viral Production

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    Collection
    ...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is...and probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular...Identity Confirmation and Sterility All of our lentiviral vectors are confirmed to have the correct integrated...transducing cells with serial dilutions of the lentiviral vector, harvesting cells several days later, ...

  10. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ... cargo into the host genome. Lentiviral-specific components Lentiviruses have gone through their fair ...for gamma-retroviruses. While not required for lentiviral production, they can be helpful for certain applications... applications and aid in ease of use. Our lentiviral guide outlines these components.     Fig....Resources on Addgene.org Addgene's most popular lentiviral plasmids Addgene's most popular retroviral plasmids...Parts of the AAV packaging plasmid Overview of Lentiviral Packaging Plasmids Adenoviral production and ...function. Lentivirus/gamma-retrovirus Lentiviruses are a class of retrovirus (so all lentiviruses are retroviruses... but not all retroviruses are lentiviruses.) This means that lentivirus and gamma-retrovirus share a lot...

  11. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References... the lentiviruses (like HIV-1) — that have been developed into lentiviral vectors. Lentiviruses are sometimes... 1: Gamma-retrovirus genome (left) and an HIV lentiviral genome (right.)  HIV seems an unlikely candidate...Addgenie Rebecca Hutcheson for her assistance with lentiviral replication.  Resources and References More ...PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of Gene-Editing Systems ...

  12. Tetracycline Inducible Expression

    Type
    Collection
    ...Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA...2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design ...-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA...rtTA3 Ptet, miniCMV Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression. See article...rtTA-Advanced H1-2O2 Stephen Elledge 111177 LT3GEPIR Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant...TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP...rtTA TRE3GS Jason Sheltzer 11651 pLVUT-tTR-KRAB Lentiviral Tet-On vector for inducible expression of transgene...

  13. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...brain region of interest. AAV or lentivirus?  Both AAV and lentiviral vectors are used for intracerebral...wider diffusion is required. On the other hand, lentiviral vectors are superior packaging vectors for larger...astrocytes, and AAV4 targets mostly astrocytes. Lentiviral vectors are pseudotyped in most cases with VSV-G...injection. Find more details about choosing AAV or lentiviral vectors in our previous blog posts.  Viral vector...1013 vg/ml (vector genomes per milliliter) and lentiviral vectors around 108 IU/ml (infectious units per...

  14. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Sakuma et al   Multiplexed Lentiviral Expression Cassettes lentiviral transduction One vector expresses...expressing the Cpf1 multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the... multiple expression platforms: transfection, lentiviral transduction, and AAV transduction.  In most ...Cpf1. This means Cpf1 can bind and cleave the lentiviral RNA, preventing packaging of the virus.  Reversing... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA...of the direct repeat protects the (+) stranded lentivirus RNA from Cpf1-mediated cleavage. This reversing...

  15. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...digestion and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs. As each..., the ligation products are a diverse pool of lentiviral plasmids. Multiple rounds of digestion and ligation...for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12 (Figure 2). Cloning from...from the pAWp28 storage vector to the pAWp12 lentiviral vector should retain the diversity in your gRNA...recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library." Nature biotechnology...

  16. Download Science-Themed Zoom Backgrounds from Addgene

    Type
    Blog Post
    ...ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some of our lentiviral plasmids ...Download the orange lentivirus background.         We also have ready-to-use lentiviral preps for COVID-...background.     If you’re working with AAV or lentivirus in your research, you might want to use one of...

  17. Mammalian RNAi Tools

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    Collection
    ...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more....shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...for cloning in shRNAs. Most plasmids are in a lentiviral backbone, with some retroviral and AAV backbone...vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    ... A novel RNA targeting system Heat-inducible lentiviral Car constructs Anti-GFP [N86/8R] ready-to-use...of print. PMID: 36690762.   Heat-inducible lentiviral CAR constructs by: Brian O'Neill  A clever system...crux of the system is a set of heat-inducible lentiviral CAR constructs, the most basic of which is depicted..., inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the...

  19. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...Base editors CRISPR toolkit for epitope tagging Lentiviral capsid-based Cas9/gRNA ribonucleoprotein delivery...toolkit   Cas9/gRNA ribonucleoprotein delivery by lentiviral capsid-based bionanoparticle Transient expression... Cas9. Recently, Baisong Lu’s lab developed a lentiviral capsid-based bionanoparticle system for packaging... aptamer binding protein for packaging into a lentivirus-like particle. Read the paper in Nucleic Acids...

  20. Lentiviral Prep Service

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    Collection
    ...available in lentiviral format....and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating...CellTag Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding...CellTag Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding...CellTag Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding... CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled ...
Showing: 21 - 40 of 187 results