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We narrowed to 82 results for: lentiviral construct

Showing: 21 - 40 of 82 results

  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed with the...CMV-TO) expression, or a promoterless construct. Pre-constructed Entry vectors containing Cas9, EGFP, ...of inducible promoter constructs. Up to 34 building blocks were used to construct 20kb long inserts. The... MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with just the...att sites are supplied as individual reporter constructs or as the fully assembled pMemoryArray.   Yang...Biotechnol Lett. 2015. 37:383-389. Toolkit for constructing fusion proteins with customizable N- and C-terminal...collection of plasmids comprising the multiple lentiviral expression (MuLE) system from the laboratory ...

  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...assembled into a final transgenesis construct. Plasmids constructed using Golden GATEway cloning have been...., BMC Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems...Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...complexity of assembling recombination of transgenesis constructs? A new cloning system combining the advantages...simplifies the cloning process for complex DNA constructs, particular for those involving recombination...Multisite Gateway cloning to generate the final construct. Up to eight entry vectors can be used for ...methods, particularly for complex transgenesis constructs. Kirchmaier et al., PLOS One 2013 Oct 7;8(10...

  3. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ... from other researchers, and cloned them into lentiviral vectors to generate a kit which is now available... cloned all the cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode ...Institute, they expect to add another 200 or 250 constructs to the kit in the next two years. References...

  4. Plasmids 101: The protein expression toolbox

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    Blog Post
    ...transiently induce expression? Check out Addgene's Lentiviral dCas9 SAM vector and Piggybac dCas9 SAM vector...promoters and modes of action Check out Addgene's Lentiviral Tet-on and AAVS1 targeted Tet-on transgene vector...Adapted from Nabet et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging ... promoter or tag prior to introduction of the construct into cells. However, if you would like tunable...Addgene website Promoter Guide CRISPR Guide Lentiviral Guide  ...mechanism to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic ...

  5. Tetracycline Inducible Expression

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    Collection
    ...Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control expression of your...Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for inducible expression of shRNA...2O2 Dmitri Wiederschain 85966 EZ-Tet-pLKO-Puro Lentiviral Tet-On plasmid for easier cloning and design ...-2O2 Cindy Miranti 104321 tet-pLKO-sgRNA-puro Lentiviral Tet-On plasmid for inducible expression of sgRNA...rtTA3 Ptet, miniCMV Howard Gu 44012 pInducer20 Lentiviral Tet-On vector for inducible expression. See article...rtTA-Advanced H1-2O2 Stephen Elledge 111177 LT3GEPIR Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant...TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP...

  6. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ... for the transgenic construct). More importantly, the expression of this construct is Cre-dependent, enabling...Figure 1: (A) Schematic of the Cre-dependent SPH construct for generating the SPH mouse. (B) AAV plasmids...by the Day Lab, who developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell...neuronal physiology. The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

  7. Viral Production

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    Collection
    ...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...determine recombination rates in FLEX/DIO/fDIO constructs. Endotoxin Endotoxin contamination in vector ...cells are transduced with serial dilutions of a lentiviral vector, incubated for 72 h, and genomic DNA is...and probes targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular...Identity Confirmation and Sterility All of our lentiviral vectors are confirmed to have the correct integrated...

  8. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction Additional... individually overexpressed and available for lentiviral packaging and delivery to target cells as a pooled...with each cell receiving only 1 TF expression construct. Cells were cultured following a differentiation... Fig. 1: Barcoded TF library was packaged in lentiviral particles and used to transduce human embryonic...

  9. Lentiviral Prep Service

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    Collection
    ...-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...available in lentiviral format....and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating...CellTag Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding...CellTag Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding...CellTag Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding... CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau...

  10. Scientists Map the SARS-CoV-2-Human Interaction Network

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    Blog Post
    ...started, here are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except...viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, all viral...projects should be fun and straight-forward. All constructs are tagged with a 2xStrep-tag for easy detection...vector background, so you can package them into lentiviral vectors if you need to. Additionally, they have...correctly-sized proteins by Western Blot for 25 constructs, and confirmed expression via mass spec for two...Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing QC and will be available shortly...

  11. CRISPR Plasmids - Empty gRNA Vectors

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    Collection
    ...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP 57818 Mammalian/Lentiviral BsmBI yes...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral BsmBI ...Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC 57829 Mammalian/Lentiviral BsmBI...62348 Mammalian/Lentiviral BbsI none S. pyogenes Puro Ochiai pSB700 64046 Mammalian/Lentiviral none S. pyogenes...57822 Mammalian/Lentiviral BsmBI none S. pyogenes EGFP Ebert pSECC 60820 Mammalian/Lentiviral BsmBI yes, cut...

  12. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...flexibility. Find the engineered prime editors here A lentiviral CRISPR knockout library that targets human epigenetic...the scale of your CRISPR screens by using this lentiviral CRISPR human genome-wide library deposited by...library delivers 2 randomly paired guides per construct and allows a reduced scale in screens without ...serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1...

  13. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post
    ... well as the viral LTRs, differ. As a result, lentiviral and γ-retroviral packaging plasmids are not interchangeable...using 293T cells This method is very similar to lentiviral packaging methods. The three components described...wildtype LTR promoter is no longer present in these constructs. Why do many γ-retroviral plasmids have similar...found within both the 5' and 3' LTRs of retro/lentiviral plasmids. U5; Unique 5'; region at the 5' end... cells a virus can infect. Note: While both lentiviruses and γ-retroviruses use the same gene products...How do engineered γ-retroviruses differ from lentiviruses? There are a few important differences between...between engineered γ-retroviruses and lentiviruses. First, these viruses were derived from different genomes...

  15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type
    Blog Post
    ...generate negative control constructs — more on this later. For more on constructing fusion proteins, check...makes it easier to generate a large number of constructs for high-throughput screening of PPIs. For example...generally involves three steps: fusion protein construction, cellular expression, and quantification (Kerppola...described in more detail below: Fusion protein construction Fusing FP fragments to your PIPs requires careful... Connection. Cellular expression Whether BiFC constructs are expressed transiently by transfection or ... comparing the expression levels between BiFC constructs and their endogenous counterparts using western...blotting. The subcellular localization of BiFC constructs should also be compared against those of their...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Howarth lab created the MBPx-SpyCatcher construct. This construct is used to produce a maltose binding protein...However, the laborious construction of repair templates and guide RNA constructs, as well as the difficult... Human Kinase Domain Constructs Kit, a library of human kinase domain constructs developed through a large-scale...the authors packaged it into lentivirus and used the resulting lentiviral library to generate a population...GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors then used...Science. 2013. PubMed PMID: 23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable... that results in the creation of many E. coli constructs with greatly varied levels of expression. The...

  17. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ... - Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...Blasticidin Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-...C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF expression pDest-565 - Gateway...for mammalian expression pLV-mCherry - 3rd gen lentiviral plasmid expressing mCherry pBad-EBFP2 - Bacterial...dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing and nondividing ...integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene expression...

  18. dTAG - You're it!

    Type
    Blog Post
    ...available from Addgene. These plasmids enable lentiviral expression of N- or C-terminally FKBP12F36V-tagged...detailed information on cloning procedures used to construct the deposited plasmids. Molecules: We synthesized...unclear which terminus should be tagged, the dTAG lentiviral plasmids can serve as a rapid means of comparing...multiple FKBP12F36V-tagged proteins using our lentiviral expression platform, to assess selective degradation...

  19. Hot Plasmids Spring 2024

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    Blog Post
    ...within the lentiviral 3’ long terminal repeats (LTR). The 3’ LTR is duplicated during lentiviral integration...license. In this iteration, the size of the lentiviral insert was minimized to reduce recombination ...III transcript, the sgRNAs were encoded on the lentiviral minus strand in the 3’ LTR. These optimizations...produced 90% accurate integration of multiplexed constructs into target genomes and a 10-fold improvement...

  20. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    .... Next, consider the expression level of your construct: would you like to stay as close as possible to...Finally, you can add bells and whistles to your construct. If you are planning to track your protein in ...basic mammalian expression system and go for a lentiviral or a retroviral backbone. This is considered ...
Showing: 21 - 40 of 82 results