Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 35 results for: lentiviral envelope plasmid

Showing: 21 - 35 of 35 results

  1. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...in weeks Figure 1: Sending out 80 FedEx envelopes in a single day -- all while maintaining social...vector background, so you can package them into lentiviral vectors if you need to. Additionally, they have...going on there!).  Find the Krogan Lab SARS-CoV-2 plasmids (Note: The Nsp6 construct is undergoing QC and... has been inundated with requests to share our plasmids with other researchers. We have been excited to...Getting our research in line, with a row of FedEx envelopes waiting to be shipped out of the Krogan lab at...Gordon) have been quite busy shipping and sending plasmids to all who request them -- in locations all over...Facebook. Find more COVID-19 and coronavirus related plasmids and resources at Addgene* Many thanks to our...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...check out Addgene's Lentiviral Guide). To produce lentiviral vectors, the lentiviral genome is distributed...required for the production of lentiviral particles.  The envelope plasmid typically encodes the vesicular... to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production protocol...All Things Lentivirus More resources on addgene.org Lentiviral Guide   Popular lentiviral vectors ...integrase-deficient lentiviral vectors, how they work, and why you might choose them over integrating lentiviral vectors...integrase-deficient lentiviral vectors, let’s briefly revisit the basic biology of lentiviral vectors (for a....  The lentiviral transfer plasmid encodes your insert of interest, flanked by long terminal repeat (...

  3. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ... called an envelope. Viruses without an envelope are referred to as naked or non-enveloped, while viruses...viruses with an envelope are called enveloped viruses (Figure 1). Like bacteria, only a tiny fraction of... Dorp, 2017). Figure 1: A non-enveloped virus (left) and an enveloped virus (right). Created using biorender.com...like baby spiders from an egg sac. (Okay, some enveloped viruses bud out, instead of lysing, but they do... particle, which retains the protein coat (and envelope), has a “gutted” genome with genes central to ...the required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors...after the viruses they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that...

  4. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    .... Packaging and Envelope plasmids provide the necessary components to make lentiviral particles. Stable...efficiency. Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you ...for in vivo viral delivery. Cas9 mRNA and gRNA Plasmids containing gRNA and Cas9 are used in in vitro ...efficiency of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR ...: Traffic Light Reporter System: Can evaluate lentiviral component delivery as well as genome repair by...User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems Check out our CRISPR Guide...

  5. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References... the lentiviruses (like HIV-1) — that have been developed into lentiviral vectors. Lentiviruses are sometimes... 1: Gamma-retrovirus genome (left) and an HIV lentiviral genome (right.)  HIV seems an unlikely candidate...host/producer cell membrane, and acquired as an envelope during exocytotic budding, as the viral particle...Addgenie Rebecca Hutcheson for her assistance with lentiviral replication.  Resources and References More ...PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of Gene-Editing Systems ...

  6. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...transfer plasmid), the envelope (Env) gene on a separate plasmid (envelope plasmid), and the polymerase ...different plasmids. For a retroviral system this looks like the genetic cargo in one plasmid (the transfer...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection... transfection of all three plasmids into a producer cell line, such as HEK-293T, production of viral particles... and Pol genes are not encoded in the transfer plasmid, the component that physically integrates into ... retroviral packaging components onto distinct plasmids for safety.    Viral cargo-specific safety...services Addgene Biosafety Guide Addgene Viral Plasmids Which Type of Virus Should I Use? Resources...

  7. Adenovirus Plasmids

    Type
    Collection
    ... Viral Plasmids Adenovirus Plasmids Adenovirus Plasmids Additional Resources: Adenovirus...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are...yeast. Two plasmids, termed a shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined...contains AdEasy-1™ plasmid Vogelstein Adenoviral Plasmids Common shuttle and adenoviral plasmids. Want an adenoviral... Venus tag; See plasmid 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular...Strain for recombination between shuttle plasmid and adenoviral plasmid Vogelstein 16399 AdEasier-1 BJ5183 ...adenoviral plasmid with your gene of interest? Search our database for adenoviral transfer plasmids that have...

  8. Plan Your Experiment

    Type
    Collection
    ...cells Packaging and envelope plasmids provide the necessary components to make lentiviral particles (for details...high efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR...to generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression...Read more about how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once...

  9. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...stranded, Parvoviridae DNA virus, packaged in a non-enveloped icosahedral capsid, that can be used to express...of both polarities are rescued from the vector plasmid and individually packaged into the AAV capsids....? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about Viral Vector Elements Resources...

  10. Lentiviral Vector Guide

    Type
    Guide
    ...second-generation lentiviral plasmids. Envelope plasmid env in trans The viral envelope gene; typically ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...distinct plasmids for safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid...), only envelope removed Envelope plasmid — contains env Figure 2: First-generation lentiviral plasmids...Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...produce lentiviral vectors, you need three (or four, for third generation) plasmids: Transfer plasmid — contains...

  11. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...generations” of plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector...capacity Packaging plasmid — contains packaging genes gag and pol Envelope plasmid — contains packaging..., similar to lentiviral packaging methods . The three plasmids described above (envelope, packaging, and...different plasmids containing the transfer, envelope, or packaging components. The transfer plasmid encodes...Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ... and integrase components. Envelope plasmid env in trans The viral envelope gene; typically vesicular ... of all gamma-retroviral plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order...

  12. Viral Vectors

    Type
    Collection
    ... to find plasmids that can be used to engineer various types of viruses. Lentivirus Plasmids | Guide Lentiviruses...for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type Visit...transgene expression. Overview of Viral Plasmids While plasmids are typically used to encode, propagate...organism. Figure 1. Using viral plasmids to generate virus. A) Viral plasmids have been engineered such that...genomes have been adapted into a plasmid-based technology, such that plasmids can be used to create viruses...researchers can introduce plasmids to a host to generate virus. Furthermore, these plasmids can be modified to...are usually divided among many plasmids, such that all of these plasmids must be present for a viral particle...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ...insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses, so they are resistant to alcohol-based...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...facilities (Biosafety Level-1). Other possible tools, lentiviruses for example, are not approved for use in vivo...Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount of immune...

  14. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...HSV can). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid ... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

  15. Plan Your Experiment

    Type
    Guide
    ...cells Packaging and envelope plasmids provide the necessary components to make lentiviral particles (for details...high efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...Additionally, many plasmids containing validated gRNAs are now available through Addgene. These plasmids contain ...particular model organism. There may not be a perfect plasmid for your specific application, and in such cases...different activators exist, including the multi-plasmid SAM system. Select Expression System To use CRISPR...to generate stable cell lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression...Read more about how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once...
Showing: 21 - 35 of 35 results