28 Hot Plasmid Technologies from 2015
Type
Blog Post
...resistance gene (missing the first two codons). Two stop codons are inserted between the truncated gene and...ligated into the MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion and providing the...bound to the mRNA since they are tethered AFTER the stop codon. Thus a non-translated mRNA will appear yellow...variety of bio-orthogonal UAAs via a re-assigned stop codon (TAG). This polyspecificity enables the site-specific...terminators and various pieces for inducible expression, loxP sites, H2B, P2A or PEST2D sequences. Several pre-constructed...