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We narrowed to 25 results for: lox-stop-lox

Showing: 21 - 25 of 25 results
  1. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...resistance gene (missing the first two codons). Two stop codons are inserted between the truncated gene and...ligated into the MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion and providing the...bound to the mRNA since they are tethered AFTER the stop codon. Thus a non-translated mRNA will appear yellow...variety of bio-orthogonal UAAs via a re-assigned stop codon (TAG). This polyspecificity enables the site-specific...terminators and various pieces for inducible expression, loxP sites, H2B, P2A or PEST2D sequences. Several pre-constructed...
  2. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These plasmids localize opsins ...pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP ... mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP ...
  3. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The...recently packaged a soma-targeted opsin from Christopher Harvey’s lab. According to their publication ...)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE...
  4. Optogenetics AAV Preps

    Type
    Collection
    ...dependent 8 Karl Deisseroth 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R..., 9, rg* Karl Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP....1 CaMKII C1V1 (t/t) mScarlet Constitutive 9 Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos...ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Christopher Harvey 108912 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-...ChrimsonR (soma-targeted) mScarlet Constitutive 1, 9 Christopher Harvey 130991 pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1...Flp dependent 8 Karl Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent...
  5. Retrograde AAV viral preps

    Type
    Collection
    ...Viviana Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses...Norepinephrine sensor Yulong Li 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a Activator,...Optogenetics Karl Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent...- pAAV SYN1 HA-hM3D(Gq) Syn Activator DREADD Christopher Richie 50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry Syn...
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