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We narrowed to 7 results for: lox-stop-lox

Showing: 1 - 7 of 7 results
  1. Cre-Lox and Other Site-Specific Recombinases

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    Collection
    ...Gene Expression: A stop codon flanked with loxP sites (often called a "lox-STOP-lox" or "LSL" cassette... gene expression to physiological levels. In lox-STOP-lox shRNA constructs, Cre expression promotes shRNA...Cre, the stop codon is excised, and gene expression proceeds. Gene Knockout: Placing the loxP sites on...shRNA Expression: Cre-lox can be used to turn shRNA constructs on or off. In floxed-shRNA constructs, Cre...Learn about Cre-Lox and other site-specific recombinase systems, how to use them to modify the genome... Plasmid Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids...Site-specific recombinase (SSR) systems, like Cre-lox, derive from fungi or bacteriophages and are powerful...
  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    Collection
    ...Expression Vectors page Cre-lox Site-specific recombination See our dedicated Cre-lox Plasmids page pBF3060 ...protein purification. Just remember to remove the stop codon for C-terminal tags and omit the start codon...including plasmids for constitutive, conditional (Cre-lox), or inducible (Tet) expression miRNA and 3' UTR ...
  3. Lentivirus Plasmids

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    Collection
    ...Dmitri Wiederschain 11578 pSico 3rd Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns...marker. See plasmid 14749 for Thy1.1 selection. Christophe Benoist and Diane Mathis 21915 Tet-pLKO-puro ...
  4. Bacterial Expression Systems

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    Collection
    ...CRISPR Plasmids and Resources page. Check our Cre-Lox Plasmids Collection for a selection of plasmids containing...Mycobacterium sp. Ashwani Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase activity Fluorescence ...benzoate (cumate) Methylobacterium extorquens Christopher Marx 44448 pLC291 pR/TetO Anhydrotetracycline...Anhydrotetracycline (aTc) Methylobacterium extorquens Christopher Marx 122635 pPEPZ-Plac Plac Lactose/IPTG Streptococcus...PcpcG2 Green Light (532 nm) Escherichia coli Christopher Voigt 164226 pSCrhaB2plus PrhaBAD Rhamnose Burkholderia...Fluorescence (GFP:mRFP1 ratio) Escherichia coli Christopher Voigt 65008 pCRISPReporter-mCherry Promoter activity...
  5. Genetic Code Expansion

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    ...challenging. The most common GCE method involves using a stop codon, such as the amber (UAG), ochre (UAA), and...Ochre (rEcΔ2.ΔA.B3.tW*) Utilizes UAA as the sole stop codon, with UGG encoding tryptophan and UAG and ... Derived from C321.deltaA, by replacing 1195 TGA stop codons with the synonymous TAA, and engineering ...Mut1-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram...Mut2-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram...
  6. Optogenetics AAV Preps

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    ...dependent 8 Karl Deisseroth 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a ChR2/H134R..., 9, rg* Karl Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a ChR2/H134R EYFP....1 CaMKII C1V1 (t/t) mScarlet Constitutive 9 Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos...ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Christopher Harvey 108912 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-...ChrimsonR (soma-targeted) mScarlet Constitutive 1, 9 Christopher Harvey 130991 pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1...Flp dependent 8 Karl Deisseroth 20949 pAAV-double floxed-eNpHR-EYFP-WPRE-pA EF1a eNpHR EYFP Cre dependent...
  7. Retrograde AAV viral preps

    Type
    Collection
    ...Viviana Gradinaru 112677 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP EF1a Color-flipping switch. Expresses...Norepinephrine sensor Yulong Li 20297 pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA EF1a Activator,...Optogenetics Karl Deisseroth 20298 pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA EF1a Activator, Cre-dependent...- pAAV SYN1 HA-hM3D(Gq) Syn Activator DREADD Christopher Richie 50458 pAAV-hSyn-DIO-rM3D(Gs)-mCherry Syn...
Showing: 1 - 7 of 7 results