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We narrowed to 466 results for: organization Addgene

Showing: 21 - 40 of 466 results

  1. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic solvents can... for use with this type of fixation*. Organic Solvents Organic solvents, generally acetone, methanol,...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...types of fixatives - chemical cross-linkers and organic solvents.  Chemical Cross-Linkers Chemical cross-linkers...quite as well as a cross-linker. This is because organic solvents can be quite harsh - lipids and soluble...disrupt your target epitope (Fig. 1). So, while organic solvents may improve antibody binding for some ...targets, they can destroy it for others. That said, organic solvents’ impact on lipids means that they are ...

  2. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...populations of transgenic laboratory organisms but not wild organisms. For example, an sgRNA-only drive ...confinement seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety... Medical School. Scientists making transgenic organisms with Cas9 should be aware of the potential hazards... can spread even when costly. If even a single organism carrying a synthetic gene drive were to escape... extreme caution. Why does making transgenic organisms with Cas9 risk creating a gene drive? The simplest...idea or wanted to build a gene drive in a model organism would find our work and consequently use these...experiments in a geographic area where escaped organisms won't be able to find mates2. For example, ongoing...

  3. Fluorescent Protein Guide

    Type
    Collection
    ...100 empty backbones. Additional Resources at Addgene Addgene Blog: Fluorescent Proteins Luciferase Plasmid... to find the plasmids that are available from Addgene's depositing scientists. Plasmid... Fluorescent Proteins: Plasmids and Resources Addgene's plasmid repository contains thousands of fluorescent... infrared tools to study molecular processes in organs and whole mammals. Michael Davidson Fluorescent...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...different organisms. There are several codons that encode the same amino acid. Each organism, however,...synthetic? How Addgene;s dataset reveals trends in biological innovation Resources on Addgene.org Check out...the genetic code is universal, many different organisms actually prefer certain codons over others for... biologist who wants to express genes from one organism in another? Let’s take a look at codon usage and...optimize codons for expression in a particular organism. Why is codon usage important? Translation and...for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization...expression by changing synonymous codons based on an organism's codon bias. The idea is that scientists can make...

  5. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  6. MAPK Plasmids

    Type
    Collection
    ...email deposit@addgene.org to be paired with an Addgene scientist. MAPK Plasmids (organized by gene) Gene... numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection for community...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  7. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  8. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...require the organism to be specified and are truly organism agnostic. Other tools require an organism to be ...only one, or a subset of organisms. In only a few cases the software is organism agnostic. Most tools are...tools will be marked as organism agnostic and have currently available organisms listed in this section...input types can be readily converted.  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends... tools also requiring that the user specify an organism. Output diversity: Different tools provide results...large genome”, and “organism biased scoring”. Trends: Many tools can handle genomes of any size and are...

  9. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide Addgene Viral Plasmids ...from organization to organization, so you’ll need to be familiar with your specific organization/department...-2 labs. When working with lentivirus, many organizations require or recommend disposable gowns over your...cabinet, even if it is not required by their organization. It’s always okay to add another level of protection..., 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher percentage of...cut. Following all sharps procedures for your organization will significantly reduce the risk of an accident...also need to familiarize yourself with your organization’s reporting mechanisms before you start an experiment...

  11. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...responsibilities Being a leader in an organization, you need to make sure the organization speaks in “one voice” whilst...great organization to develop these leadership skills in particular because every year it organized a “Committee...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ... achieved by volunteering for a professional organization or a scientific society in your field. My leadership... in the long run was most beneficial for the organization as a whole. I came to understand that not only...only was I growing as a leader but the organization was growing too and with that would come necessary... had to be professional as I represented the organization, not just myself as a scientist. I learnt to...

  12. Engaging with science and society at pgEd

    Type
    Blog Post
    ... Resources on Addgene.org Learn how Addgene engages with the community on our Addgene gives back page...different organizations and individuals, to identify ways of collaborating, and to organize events that...professional development (PD) workshops for teachers; organizing Congressional briefings in Washington DC; convening...Hollywood; and working directly with community organizations, libraries, youth groups, and faith institutions...studies (STS). I also worked with a number of organizations in public engagement efforts, including giving...middle and high school students. One of these organizations was pgEd. After obtaining my PhD and working...audiences. Many universities or cities have organizations (often volunteer-based) that put on public lectures...

  13. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    ...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...us know about other organisms we should include by emailing us at help@addgene.org. If you are looking...Addgene will be at the American Society for Microbiology Microbe meeting here in Boston next week - be... and say hello! Just in time for this meeting, Addgene is launching a new resource page for the Microbiology...plasmids used in E. coli and yeast, you’re in luck! Addgene has so many of these resources that we created ...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...technology, sign up to write about it on the Addgene blog. ...

  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...Conjugation, and Transfection Resources on Addgene.org Addgene's Synthetic Biology Collection...Here at Addgene, we often refer to plasmids as lab or experimental tools. They certainly are very handy... a variety of functions that allow their host organisms to thrive in competitive environments. The genes...the competition, and Penicillium isn’t the only organism that knows it. Some bacteria possess plasmid-borne...encode genes that allow the host to infect other organisms. If you’ve ever wondered why E. coli is safe to...means to deliver DNA and other molecules to host organisms. For instance, vectors derived from the Agrobacterium...like by living where no one else wants to. Some organisms manage this with plasmids that allow them to eat...

  15. Plasmid Collections

    Type
    Collection
    ... Addgene Homepage Plasmid Collections Addgene Plasmid Collections Addgene's repository contains...Browse Addgene’s curated collections of plasmids, kits, and pooled libraries. Find tools for CRISPR, ...Collections A modular set of plasmids from a depositor organized by a common use. Click here to view all collections...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  16. Neurodegeneration Research Collection

    Type
    Collection
    ...out about the plasmid collections available at Addgene that may be useful for neurodegeneration research... Neurodegeneration Research Collection Addgene has assembled the following to help you find information... some of the relevant collections available at Addgene. Amyotrophic Lateral Sclerosis Amyotrophic lateral...Tools Find CRISPR pooled libraries available from Addgene, sortable by type of genetic modification and ...Dec 5. See More Antibodies Find information on Addgene's collection of plasmid-based antibodies, including...More Fluorescent Proteins Find information on Addgene's collections of fluorescent proteins, including...monitor structural transitions in protein self-organization. Wan et al. Cell Rep Methods. 2022 Mar 28. Follow...

  17. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  18. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  19. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists...conference here. The Future of Research conference is organized by postdocs for postdocs. I am delighted to see...importance of the topics to be discussed, the organizers are building valuable skills and meeting new ...Students also partner with independent career organizations to get the grants and programs they need such...offices in the last few years. However, some organizations are woefully behind and some just don't have...Symposium on the Future of Science: A postdoc-organized symposium on the future of the scientific endeavor...

  20. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids and resources...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...
Showing: 21 - 40 of 466 results