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Addgene

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We narrowed to 449 results for: organization Addgene

Showing: 21 - 40 of 449 results

  1. CRISPR Plasmids - Activate Gene Expression

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    ...upregulating gene expression in various model organisms....out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  2. Fluorescent Protein Guide: In Vivo Imaging

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    ... or protein-protein interactions in deep-tissue organs and whole mammals. Genetically encoded fluorescent...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...gene expression Lentiviral backbones made by Addgene Addgene has an extensive collection of plasmids for...A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function...to consider. Here is a guide to a selection of Addgene's empty vector backbones. For the most part, we ...promoter that will be functional in your host organism. Host Relevant Promoters Representative Empty ..., or zebrafish expression GST plasmids made by Addgene MBP Protein purification pET28-MBP-TEV - Bacterial...efficiency, but do NOT integrate into the host genome Addgene has an extensive collection of plasmids for packaging... shRNA expression Retroviral backbones made by Addgene Adenoviral High transduction efficiency, but do...

  4. AAV Packaged on Request

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    ...experiments as soon as they receive their AAV prep from Addgene — eliminating the steps to produce, purify, and...this service. Select any eligible plasmid in the Addgene repository in your choice of seven serotypes and...more about AAV serotypes and tissue tropism on Addgene's blog . All-in-One Pricing The price for our Packaged...production Density centrifugation purification Addgene’s comprehensive quality control process: Titering...Approval 1+ business days, varies by requesting organization Viral vector preps remain subject to the terms...remember to share your protocols and results through Addgene’s Data Hub . By contributing your AAV vector experience...let you know whether the item can be packaged. Addgene does not perform functional or in vivo testing....

  5. Bacterial Expression Systems

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    .... Additional Addgene Genome Engineering Resources Learn more about CRISPR with Addgene's CRISPR Guide ...Escherichia coli is the most widely used bacterial organism due to its ease of use, speed of growth, and well...are also being used for their distinct features. Addgene distributes many plasmids that can help researchers...MalE, OmpA ID Plasmid Promoter Tags PI Additional Addgene Protein Purification Resources The Structural Genomics...localization to the periplasm. The pCri System (Addgene #1000000058) plasmids can be utilized for heterologous...and Pichia pastoris . The EcoFlex MoClo Toolkit (Addgene #1000000080) features a library of promoters and...Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli Strains for Protein Expression Protein...

  6. mTOR Pathway

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    ...Addgene's plasmids for the mTOR pathway. enhanced signaling of mTOR, a key metabolic regulator, correlates...mTORC2 influences cell survival and cytoskeletal organization. Cancer Impact Enhanced signaling through either...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  7. Viral Vectors

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    ...List of viral vector plasmids available at Addgene and other related resources. ...Viral Plasmids and Resources You May Also Like... Addgene's Viral Service Biosafety Guide Blog: Plasmids 101... for select plasmids are now available through Addgene, learn more here . Browse Viral Plasmids by Type... this genetic material to specific cells in an organism. Figure 1. Using viral plasmids to generate virus...general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide . References Cohen S,...

  8. CRISPR Pooled gRNA Libraries

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    ...particular organism, and the library is only compatible with cells derived from that organism. All of our...pick a library. The pooled CRISPR libraries in Addgene’s collection differ based on some important criteria...filtered list of the CRISPR pooled libraries in Addgene's collection. Library Type Activation Barcode Knockout...

  9. Fluorescent Protein Guide: FRET

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    ...Addgene's collection of plasmids suitable for FRET experiments to measure protein interactions or conformation... not required to be fluorescent. A variety of organic dyes and fluorescent proteins have been developed... molecules in either of these types of studies Addgene has a collection of plasmids suitable for creating...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  10. Five Popular Model Organisms, Part 2

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    Blog Post
    ...E-coli-as-a-Model-Organism.aspx. Additional resources on the Addgene blog: Read more about model organisms in the...covered five model organisms in a previous blog post, but here are five more model organisms that have left...emerging model organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia...as a model organism for over 60 years with a status as the most studied prokaryotic organism. With its ...learned more about popular model organisms in the lab, check out Addgene’s plasmid collections to help start...Undervalued Model Organism to Study and Model Human Genetic Disease. Cells Tissues Organs, 205(5–6), 303–...and molecular tools. One such tool is the model organism, an essential aspect of biological research that...

  11. Five Popular Model Organisms

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    ...Lukas Morgan Model organisms are vital tools used by researchers around the globe. These organisms share... find these plasmids at Addgene. Another advantage of mice as model organisms is that they are genetically...one of the simplest eukaryotic organism, is a commonly used model organism in scientific research. Yes, ...resources check out Addgene’s Worm Expression page. Looking for more model organisms? Check out Part two...model organisms series, and read about new systems being developed for emerging model organisms! References...this blog post, we’ll cover five popular model organisms, but there are many more out there. The house... start with the most preferred mammalian model organism of many researchers: the mouse (Mus musculus)....

  12. Transferable Skills Guide: External Collaborations

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    ...to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate and thoughtful...opportunities within an organization but outside your comfort zone As part of my position at Addgene I am now involved... outside an organization The next step is to expand your efforts beyond your organization and/or scientific...important? In my current job at Addgene I work with people from various organizations in science and beyond. For... Science Training Resources on Addgene.org Check Careers at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly...

  13. The MTA at Addgene

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    ...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority ...Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations. Over the last twenty...differs.  When you deposit a plasmid with Addgene, your organization’s tech transfer office is sent a deposit.... In signing this agreement, your organization agrees to let Addgene receive, store, and distribute your...recipient organization is sent the MTA to sign. Once they've signed and returned the MTA, Addgene can move...distribution for their plasmids. MTAs at Addgene Since Addgene was founded in 2004, we’ve handled the majority...If you’ve deposited or requested plasmids from Addgene, then you’ve been involved in the material transfer...

  14. Plasmids 101: Codon usage bias

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    ...genetic code is used by most organisms on Earth, but different organisms have different preferences for...codons are used preferentially in which organisms - organisms have codon usage bias. You can find many...ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene that’s been codon ...peptide of interest and produce that peptide in your organism of choice. Unfortunately, codon preferences make...and expect your sequence to express well in any organism. So what are the evolutionary constraints that... do about them? Read on to find out!   Why do organisms have different codon usage biases? The reasons...reasons for varied codon preferences among organisms aren’t completely understood, but some possible reasons...

  15. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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    ...Resources on Addgene.org Check out Addgene's synthetic biology page Search for a plasmid Find Addgene's most ...coming to the Addgene website in search of plasmids. Now, scientists are beginning to see Addgene as a large...was moved exactly as is from one organism to a distantly related organism, the gene may not express well...distantly related organisms. In 2006, less than 1% of the genes deposited at Addgene were synthetic. But...or intentional release of engineered organisms. “Is [the organism] engineered? Where did it come from?...large data set. Addgene has over 65,000 plasmids in the repository, each verified by sequencing, which makes...this lies in the codon usage biases of different organisms. Different species have different preferences ...

  16. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ...on the Addgene blog Check out our other model organism blog posts: Five Popular Model Organisms, Part...this programs via Addgene. New organisms for genotype to phenotype studies New organismal systems described...diverse organisms. Methods for genetic manipulation still need to be developed for each organism, but researchers...and share all of these new organisms continues to be challenging. Organism specific viral vector systems...and shared for many different organisms. Do you have a favorite organism you study in the lab? Let us ... work but never get to actually manipulate the organism they study (unless if you count normal human interaction...spiny purple sea urchin. It’s not a standard model organism, but it was easy to harvest eggs and sperm, mix...

  17. Plan Your Experiment

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    ...AAV size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service....researchers can adapt these experiments for different organisms. This page provides a general framework to get...principles also apply to using CRISPR in other organisms. As with most experiments, you first need to have...appropriate left and right homology arms. See Addgene's HDR blog post for more details. In some cases,...the reference sequence you used for gRNA design. Addgene offers many plasmids containing validated gRNAs...plasmid in question (see CRISPR protocols from Addgene depositors ). Browse empty gRNA expression vectors...you may need to look into other options. Browse Addgene's collection of CRISPR plasmids . Delivery Using...

  18. TALEN Plasmids and Kits

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    ...plasmid kits and accessory plasmids available from Addgene for generating your own specific TALE plasmids.... TALEN Plasmids and Kits You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc ...amino acid repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying...Golden Gate cloning method. Validated in multiple organisms. Dan Voytas Adam Bogdanove Joung Lab TAL Effector...Takashi Yamamoto Additional Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...reagents to use - organic solvents and detergents.  Organic Solvents  As mentioned above, organic solvents can... for use with this type of fixation*. Organic Solvents Organic solvents, generally acetone, methanol,...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...types of fixatives - chemical cross-linkers and organic solvents.  Chemical Cross-Linkers Chemical cross-linkers...quite as well as a cross-linker. This is because organic solvents can be quite harsh - lipids and soluble...disrupt your target epitope (Fig. 1). So, while organic solvents may improve antibody binding for some ...targets, they can destroy it for others. That said, organic solvents’ impact on lipids means that they are ...

  20. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...populations of transgenic laboratory organisms but not wild organisms. For example, an sgRNA-only drive ...confinement seeks to keep the organisms in the laboratory. It varies by organism, but your local biosafety... Medical School. Scientists making transgenic organisms with Cas9 should be aware of the potential hazards... can spread even when costly. If even a single organism carrying a synthetic gene drive were to escape... extreme caution. Why does making transgenic organisms with Cas9 risk creating a gene drive? The simplest...idea or wanted to build a gene drive in a model organism would find our work and consequently use these...experiments in a geographic area where escaped organisms won't be able to find mates2. For example, ongoing...
Showing: 21 - 40 of 449 results