We narrowed to 166 results for: pcr

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Learn how to verify indels, deletions, and homology directed repair (HDR) events in your CRISPR modified cell lines.

Learn about antibody engineering, including what it is and why we like it. Deep dive into antibody directed evolution techniques, and learn about Addgene's partnership with the Institute for Protein Innovation (IPI).

Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Overview of Addgene's viral production methods and quality control for lentivirus and AAV.

Ever wondered how Addgene makes our viral preps? We explain it all here!

Find some tips and tricks for using CRISPR for gene editing.

Learn how to use and apply NEBuilder HiFi DNA Assembly Technology from New England Biolabs for quick and accurate plasmid cloning.

Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.

Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The collection can be used for screening transcription factors for cellular programming or cancer drug resistance.

With the limitations of qRT-PCR based COVID-19 testing, scientists develop CRISPR-based diagnostic tools for COVID-19.

Learn how various lab techniques have evolved but still remain relevant in many research arenas today.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

Blog editor Tyler Ford runs down some of the best posts from August 2016. NgAgo, site directed mutagenesis, and much more!

An exploration of how the Trimmer lab developed their Addgene-deposited collection of recombinant antibodies.

Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Read about Gladys Kaba, the 3rd recipient of the Addgene/Seedinglabs Plasmid Grant, who will be using plasmids as positive controls in cervical cancer screens.