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We narrowed to 73 results for: retroviral vectors

Showing: 21 - 40 of 73 results

  1. Plasmids 101: Using Transposons in the Lab

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    ...integrates in human cells at rates similar to retroviral vectors. The hyperactive version of the SB transposase...integration efficiency in human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three different ...transfer Transposons are an alternative to viral vector delivery of transgenes, like for iPSC reprogramming... piggyBac, which is a big advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus...into new target sites. This is similar to how retroviruses, like HIV, replicate. Class I TEs do not encode...

  2. Viral Vectors 101: Viruses as Biological Tools

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    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...The most well-known retrovirus is HIV. Lentiviruses are a genus of the retroviral family that can infect... Viruses are essentially DNA (or RNA) delivery vectors, so what makes them better than the alternatives...measures in a separate, upcoming post. If a viral vector seems like the right approach for your experiment...experiment?           Click Here to Find Viral Vector Resources at Addgene! References and Resources... J, et al. “Altering the tropism of lentiviral vectors through pseudotyping.” Curr. Gene. Ther. 5 (2005...types of viruses used in mammalian research: 𝛄-retrovirus, lentivirus, adenovirus, and adeno-associated...

  3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...body and thereby correct the mutations using retroviral vectors. However, many diseases require techniques...makes AAV vectors especially amenable to correcting disease-associated mutations. AAV vectors maintain... Check out our Viral Vectors Featured Topic Page Learn about CRISPR-AAV vectors Learn about Addgene AAV...target cells has been challenging. A variety of vector delivery technologies have been investigated including...usability in clinical settings. On the other hand, vectors derived from AAV have recently gained popularity...profiles as well as other benefits. What makes AAV vectors great gene delivery tools? There are a variety ... like adenovirus for productive infection. AAV vectors are engineered to provide additional safety benefits...

  4. 5 Tips for Troubleshooting Viral Transductions

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    .... Viral vectors undergo DNA rearrangements. Depending on the composition of the viral vector and the ...about Lentiviral Vectors Additional Resources on the Addgene.org Check out Our Viral Vector Guide Pages ... one point or another. After amplifying a viral vector, collecting virus, and transducing target cells...the repeat sequences present in the LTR, the vector may undergo genomic rearrangements during replication...genomic rearrangements, try amplifying the viral vector with bacteria designed to minimize rearrangements...have low titers (which is unavoidable for some vectors) you can concentrate your virus by ultracentrifugation...antibiotic whose resistance gene is encoded in the viral vector. This antibiotic selection can be performed approximately...

  5. Viral Vectors 101: Pseudotyping

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    ...lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using viral...Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods ...lentivirus, retrovirus, and rabies virus. How to pseudotype To pseudotype your viral vector, you use the...et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins have...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...Sometimes this is due to the cells that the viral vectors were produced in. For example, production of vesicular...another virus with rabies glycoprotein. Lentiviral vectors are also great at infecting neurons and cause minimal...

  6. Getting the Most from Your Lentiviral Transduction

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    Blog Post
    ...conditions on the production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development...About Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional...“Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology Advances ... by the envelop protein. Many times lentiviral vectors are engineered with non-native envelopes, a process...titration of pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID...Reiser, J. “Altering the tropism of lentiviral vectors through pseudotyping.” Current Gene Therapy 5.4...of the transductional efficiency of lentiviral vectors: effect of sera and polycations.” Molecular Biotechnology...

  7. Plasmids 101: Terminators and PolyA signals

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    ...Enhances Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID: ...terminator will suffice. Many commercial expression vectors use double terminators to reduce unwanted translation... handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...

  8. Retrovirus Plasmids

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    ...Popular γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral plasmid ...Viral Plasmids γ-Retroviral Plasmids γ-Retroviral Plasmids Additional Resources: γ-Retrovirus Guide Biosafety...Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus) is an RNA virus comprised...FAQ) about retrovirus Popular γ-Retrovirus Envelope and Packaging Plasmids Common γ-retroviral envelope ...mutagenesis). Read our γ-Retrovirus Guide for more information about: γ-Retroviral packaging systems Frequently...Learn about retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids...your gene of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid...

  9. Viral Vector Guides and Plasmids

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    ...capacity. Gamma-retroviral Vector Guide | Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred...Guides Viral Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral...expression. Retroviral vectors are popular for making stable cell lines or clinical use. Adenoviral Vector Guide...Viral Vectors Resources Viral vectors are popular tools for delivering genetic information to cells. These...and Plasmids Lentiviral Vector Guide | Lentiviral Plasmids Lentiviral vectors are versatile tools because...the host genome. AAV Vector Guide | AAV Plasmids Adeno-associated viral (AAV) vectors are commonly used ...referred to as just retroviral vectors, have a relatively high transduction efficiency and only infect dividing...

  10. Plasmids 101: The Promoter Region – Let's Go!

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    ...lentiviral and retroviral constructs and we will discuss these in a future post on viral vector parts. Promoter... Can be induced by IPTG. Commonly found in pET vectors. Very tightly regulated by the lac operators. Good...-inducer fucose Weaker. Commonly found in pBAD vectors. Good for rapid regulation and low basal expression...Browse All Plasmids 101 Posts Learn about Mammalian Vectors Read about Reporter Gene like Luciferase and GFP...

  11. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post
    ....g. Mammalian) Vector Type: What type of experiment are you planning? (e.g. Retroviral) Species: Specify...but I can’t browse through 300+ plasmids. Under ‘Vector Types’ I selected ‘Lentiviral’ which eliminates...Identify a specific marker protein Use: Similar to “Vector Type” you can filter by experimental purpose (e.g...

  12. PiggyBac-ing Through the Genome Editing Field

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    ...quickly transfer a DNA sequence from the transposon vector to one of many TTAA sequences distributed throughout...encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful resource....edit of interest. With the larger piggyBac™ donor vector, it is possible to make the ITR and selection cassette...splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. ...Transposase Read up on Genome Engineering with FLEx Vectors  Learn how Homology Directed Repair Is Used for...Genome Editing Resources on Addgene.org Find Vectors with the piggyBac™ Transposon Catch up on Everything...CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ...

  13. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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    ...compatible Y2H vector - BD pRPR1_gRNA_handle_RPR1t - gRNA expression empty vector pFA6a vectors - Epitope ...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...pFastBac Dual LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab ...expression vector with N- or C-terminal HA tag pInducer20 - Tet-inducible HA-tagged lentiviral vector for ORF...Gateway destination vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal...-1-3xMyc - Bacterial vector for Myc tag pETcon(-) - Yeast surface display vector with a C-terminal Myc...His-tagged versions of pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent...

  14. Hot Plasmids: Spring 2025

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    ...fusions for membrane display, they recommend the NGR vectors for most experiments. These new tools promise to... toolkit is the empty plasmid backbone: the DNA vector that contains everything needed to replicate inside... Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing...successful experiments or therapies. Using AAV vectors with enhancers that drive cell-type-specific expression...colleagues characterized a series of new enhancer-AAV vectors targeting specific classes of cortical and striatal...

  15. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ...the vector backbone you cloned your insert into and fill out the appropriate boxes with the vector backbone...backbone manufacturer, vector backbone size, total size of your vector + insert, any modifications you ...provide any full or partial plasmid sequences and any vector maps. The more sequence data available, the better... was a part of the insert (when cloned into the vector backbone). If your plasmid has more than one insert...you made to the backbone, and the vector type. Pro Tip! Reference the Addgene ID in the "Backbone Manufacturer...enter "Feng Zhang (Addgene plasmid #52961)".   The vector type should describe how your plasmid is intended...in E. coli, then this is a Bacterial Expression vector. If your plasmid can be classified in multiple ...

  16. SARS-CoV-2 Pseudotyped Virus

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    ...Lentiviral reporter vector expressing firefly luciferase. pCMV-FLuc - Retroviral reporter vector expressing firefly...Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter vector expressing...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...-Luc2-IRES-ZsGreen-W - Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen. NL4-...3 mCherry Luciferase - Lentiviral dual reporter vector expressing mCherry and firefly luciferase. pLentiEGFPdestablized...coronaviruses (184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...

  17. Five Popular Model Organisms

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    ...Zuber et al., 2009). By injecting mice with retroviral vectors that harbored different mutations found in...Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally control gene expression...

  18. Plasmids 101: Expressing shRNAs for RNA Interference

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    ...processing Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA...expressed constitutively, either in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently...cloning strategies for constructing shRNA expression vectors. BMC Biotechnology, 6(1). https://doi.org/10.1186...

  19. Plasmids for Stem Cell Research

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    ...lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral vector for "hit...MMLV-derived Retrovirus Human Yamanaka factors for generating human iPS cells; retroviral expression of...MMLV-derived Retrovirus Mouse Original set of Yamanaka factors for generating mouse iPS cells; retroviral expression...Lentivirus Human Single polycistronic lentiviral vector for the expression of human Oct4, Klf4, Sox2, and...polycistronic, doxycycline-inducible lentiviral vector for the expression of human Oct4, Klf4, c-Myc, ...transcriptional activating fusions in single lentiviral vectors for human iPSC generation OCT4 and SOX2 Work as...Polycistronic, doxycycline-inducible lentiviral vectors for the expression of mouse Oct4, Klf4, c-Myc, ...

  20. Luciferase Plasmid Collection

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    ...cells Peter Cockerill 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...Groskreutz 18760 MSCV IRES Luciferase Firefly Retroviral expression of firefly luciferase and gene of ...Scott Lowe 21375 pHIV-Luciferase Firefly Lentiviral vector for insertion of your gene of interest upstream...and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions upstream...212935 pGL4.84(hRlucCP/Puro) RapidResponse™ Renilla Vector for investigating regions controlling transcription... Pete Stecha 212933 pGL4.82(hRluc/Puro) Renilla Vector for investigating regions controlling transcription...pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic promoters. Daniel...
Showing: 21 - 40 of 73 results