...this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) with AAV vectors for efficient...and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9. These nucleases should...that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies...

...from Addgene's viral service encoding tet-off transactivators and tools for affinity purification (TRAP)....Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell ...Cell Ablation Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...ihSyn) Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...

... thanks for our guest blogger, Gairik Sachdeva! Gairik Sachdeva is a Ph.D. in Bioengineering and works...This post was contributed by guest blogger Gairik Sachdeva. Healthcare consulting is a fast-paced field, ...

.... For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it...Campylobacter jejuni (CjCas9) is even smaller than SaCas9 and is also compatible with AAV delivery. NmCas9...Addgene Blog  Read more about Cpf1 Read more about SaCas9 and AAV Check out our CRISPR Featured Topic Page...

...non-temperature-sensitive replicon, and 3) added the sacB gene for counterselection to yield pEcCas. In pTargetF...growth of its E. coli host, and the addition of a sacB gene renders it beneficial for plasmid clearance...in all three plasmids. pCutamp is then removed by sacB counterselection.  The MUCICAT toolkit includes...

...TetR On Geley Transactivators (tTA or rtTA) Find a construct that expresses the transactivator for your tetraycline... gene expression, a tetracycline-controlled transactivator (tTA) was developed. tTA was created by fusing...reduced gene expression. This idea of a hybrid transactivator was initially used with the lac system. Tetracycline... induction, rather than repression. The new transactivator rtTA ( r everse t etracycline-controlled t ...gene of interest with Strep-Tag and Tet-On 3G transactivator, creating an auto-regulated Tet-On 3G System... Lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, ...

...this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system is regulated... in Molecular Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA ...

...electroporation. Then at your preferred time points, sacrifice the animals and fix the samples. When you use ...AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will have to wait...

... tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041...

...primarily pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features...required for the replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous...

...biochemistry department at the University of Massachusetts Medical School. We are working with postdocs...to hold. There are institutions in Boston - Massachusetts General Hospital, Mass Eye and Ear Infirmary...

...by Kivanc Birsoy. Find the library here A new SaCas9 ABE variant (microABEI744) has improved on-target...footprint compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable...

...CRISPR. AAV transduction Only compatible with SaCas9 (packaging limit ~4.5kb). CRISPR elements are inserted...AAV particles. Transient or stable expression of SaCas9 and/or gRNA. Infects dividing and non-dividing ...

...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be...

...by Kusumika (Kushi) Mukherjee, a Postdoc at Massachussetts General Hospital. A little over a decade back...Mukherjee is currently a postdoctoral fellow at Massachusetts General Hospital with research interests in ...

... of the fly (Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic....5 (2011): 190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression...

...et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in ... by the fact that even small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all...

...pharmaceutical industry, depend on the yeast, Saccharomyces cerevisiae, as a key host for the production... of dCas9-VPR plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression...designed to complement unused auxotrophies in Saccharomyces strains by compensating for histidine (HIS3)...

...photoswitchable spCas9, dCas9, and S. aureus Cas9 (saCas9) proteins, showing the versatility of this approach...This study generated the first optically active saCas9 protein. Stop light! Photoactive anti-CRISPRs ...

...Features 85169  pJL-SaBE3  SaCas9 (NNGRRT)    85170  pJL-SaKKH-BE3  SaCas9 (NNGRRT)    85171  pBK-VQR-BE3...