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We narrowed to 129 results for: sacs

Showing: 21 - 40 of 129 results

  1. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...post. Fatima Enam: Biosensors for human milk oligosaccharides Fatima Enam is currently a graduate student...fluorescent protein-based biosensors for Human Milk Oligosaccharides (HMOs). HMOs are thought to play an important...Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent Proteins Use Light to Control ...

  2. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Tales of Music and the Brain by Oliver Sacks "Neurologist Oliver Sacks examines the relationship between the...therapeutic properties it has. As with other works of Dr. Sacks, Musicophilia includes case studies of relevant ...

  3. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) with AAV vectors for efficient...and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9. These nucleases should...that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies...

  4. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...non-temperature-sensitive replicon, and 3) added the sacB gene for counterselection to yield pEcCas. In pTargetF...growth of its E. coli host, and the addition of a sacB gene renders it beneficial for plasmid clearance...in all three plasmids. pCutamp is then removed by sacB counterselection.  The MUCICAT toolkit includes...

  5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    .... For example, Cas9 from Staphylococcus aureus (SaCas9) is about 1 kilobase smaller than SpCas9, so it...Campylobacter jejuni (CjCas9) is even smaller than SaCas9 and is also compatible with AAV delivery. NmCas9...Addgene Blog  Read more about Cpf1 Read more about SaCas9 and AAV Check out our CRISPR Featured Topic Page...

  7. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...this time using the Staphylococcus aureus Cas9 (SaCas9). This AAV-based genome editing system is regulated... in Molecular Neuroscience Find the AAV-based SaCas9 genome editing plasmids String assembly gRNA ...

  8. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https:...

  9. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...electroporation. Then at your preferred time points, sacrifice the animals and fix the samples. When you use ...AAV titer for the best results. The timing of sacrifice when using Tetbow AAVs - You will have to wait...

  10. Microbiology Resources

    Type
    Collection
    ...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...

  12. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...primarily pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features...required for the replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous...

  14. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...by Kivanc Birsoy. Find the library here A new SaCas9 ABE variant (microABEI744) has improved on-target...footprint compared to current N-terminal linked SaCas9 ABE variants. It's also one of the smallest AAV-deliverable...

  16. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...under the control of an inducer, i.e. tet-off transactivator (tTA), is injected at a high dose to maximize...An AAV expressing the inducer (i.e. tet-off transactivator) is co-injected. The dose of this AAV can be...

  18. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...et al 2017 use a dual vector system composed of SaCas9 and a gRNA in one AAV and a repair template in ... by the fact that even small Cas9 homologs like SaCas9 and their requisite gRNAs take up essentially all...

  19. Five Popular Model Organisms

    Type
    Blog Post
    ... of the fly (Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic....5 (2011): 190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...pharmaceutical industry, depend on the yeast, Saccharomyces cerevisiae, as a key host for the production... of dCas9-VPR plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression...designed to complement unused auxotrophies in Saccharomyces strains by compensating for histidine (HIS3)...
Showing: 21 - 40 of 129 results