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Showing: 1 - 20 of 22 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Dreyfuss 71875 pET-Sac-Abeta(M1-42) APP T7 Alzheimer's Dominic Walsh 71876 pET-Sac-Abeta(M1-40) APP T7...Beutler 127151 pET-Sac-Abeta(MC1-42) APP T7 Alzheimer's James Nowick 127152 pET-Sac-Abeta(MC1-40) APP ...Ataxia telangiectasia Charles Swanton 173759 pET-Sac-Abeta(M1-42/C18C33) APP T7 Alzheimer's James Nowick... GST tac Parkinson's Sachdev Sidhu 109964 pHH0103_VCP_AAA VCP GST tac ALS Sachdev Sidhu 110028 pHH0103...Possemato 103913 HTRA2_PDZ_1 HTRA2 GST tac Parkinson's Sachdev Sidhu 104448 pEGFPC1-hVAP-B VAPB GFP, HA CMV ALS...pHH0103_PFN1_Profilin PFN1 GST tac ALS Sachdev Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ...from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single...61591 : PX601; CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592...single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons...61592 : PX600; CMV-driven SaCas9 60957 : PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression...for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: These plasmids contain two ...two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a...

  3. AAV Molecular Tools

    Type
    Collection
    ...from Addgene's viral service encoding tet-off transactivators and tools for affinity purification (TRAP)....Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell ...Cell Ablation Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible tools...tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible expression...CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...ihSyn) Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...TetR On Geley Transactivators (tTA or rtTA) Find a construct that expresses the transactivator for your tetraycline... gene expression, a tetracycline-controlled transactivator (tTA) was developed. tTA was created by fusing...reduced gene expression. This idea of a hybrid transactivator was initially used with the lac system. Tetracycline... induction, rather than repression. The new transactivator rtTA ( r everse t etracycline-controlled t ...gene of interest with Strep-Tag and Tet-On 3G transactivator, creating an auto-regulated Tet-On 3G System... Lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, ...

  5. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 2004 Jun;21(8):661-70. doi...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact. 2013 Oct 25;12...

  6. Microbiology Resources

    Type
    Collection
    ...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...

  7. CRISPR Guide

    Type
    Collection
    ...enzyme, additional Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence...meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1...

  8. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...receptor Rat Mouse IgG2a 188151 Anti-Fig4/Sac3 [N202/7R] Fig4/Sac3 Mouse Mouse IgG2a 188153 Anti-Cav1.2 Ca2...Chapsyn-110 Rat Mouse IgG2a 220408 Fig4/Sac3 [N202/38R] Fig4/Sac3 Mouse Mouse IgG2a 220409 TRIP8b (constant...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...PLoS One. 2014 Jun 5;9(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic...sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels...

  11. AAV Viral Preps

    Type
    Collection
    ...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation Monosynaptic...

  12. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...sgRNA). While the dCas9 protein provides the transactivation activity, the sgRNA component provides the ...

  13. TALEN Guide

    Type
    Collection
    ...Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN...

  14. Lentiviral Prep Service

    Type
    Collection
    ...Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID...

  17. Plan Your Experiment

    Type
    Collection
    ... Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects dividing and non-dividing cells...

  19. Bacterial Expression Systems

    Type
    Collection
    ...cloning, and a "stuffer" fragment that includes the SacB gene, allowing for negative selection on 5% sucrose...

  20. Genetic Code Expansion

    Type
    Collection
    ...azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio 127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted...
Showing: 1 - 20 of 22 results