We narrowed to 18 results for: sacs
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TypeCollection...Dreyfuss 71875 pET-Sac-Abeta(M1-42) APP T7 Alzheimer's Dominic Walsh 71876 pET-Sac-Abeta(M1-40) APP T7...Beutler 127151 pET-Sac-Abeta(MC1-42) APP T7 Alzheimer's James Nowick 127152 pET-Sac-Abeta(MC1-40) APP ...Ataxia telangiectasia Charles Swanton 173759 pET-Sac-Abeta(M1-42/C18C33) APP T7 Alzheimer's James Nowick... GST tac Parkinson's Sachdev Sidhu 109964 pHH0103_VCP_AAA VCP GST tac ALS Sachdev Sidhu 110028 pHH0103...Possemato 103913 HTRA2_PDZ_1 HTRA2 GST tac Parkinson's Sachdev Sidhu 104448 pEGFPC1-hVAP-B VAPB GFP, HA CMV ALS...pHH0103_PFN1_Profilin PFN1 GST tac ALS Sachdev Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...
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Tetracycline Inducible Expression
TypeCollection...generations of transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter...Vogelstein Transactivators (tTA or rtTA) Find a construct that expresses the transactivator for your tetracycline...response element; tTA: tetracycline-controlled transactivator (fusion of TetR with VP16 transcriptional activation...domain); rtTA: reverse tetracycline-controlled transactivator (fusion of mutated TetR with VP16 transcriptional...the Tet-Off system. A tetracycline-controlled transactivator (tTA) was created by fusing TetR with the activation...tetracycline. A new reverse tetracycline-controlled transactivator (rtTA) was created by fusing rTetR with VP16.... These include: Tet-On Advanced : improved transactivator (rtTA-Advanced, also known as rtTA2 S -M2) ... -
AAV Molecular Tools
TypeCollection...from Addgene's viral service encoding tet-off transactivators and tools for affinity purification (TRAP)....Packaging Service: Molecular Tools Tetracycline Transactivators Affinity Purification Neurophysiology Cell ...Engineering Labeling Overexpression Tetracycline Transactivators and Inducible Tools These AAV encode tetracycline-inducible...-inducible tools/controls and tetracycline transactivators that can be used with tetracycline (tet)-inducible...CAG-driven, constitutive Expression of the tet-off transactivator (tTA) 2 Gradinaru 99120 pAAV-ihSyn1-tTA Inducible... promoter (ihSyn) Expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified...ihSyn) Cre-dependent expression of the tet-off transactivator (tTA) with a positive feedback loop for amplified... -
Zhang Lab CRISPR Page
TypeCollection...from S. aureus (SaCas9) that has been human codon-optimized, and packages both the SaCas9 gene and its single...61591 : PX601; CMV-driven SaCas9; U6-driven sgRNA 61593 : PX602; TBG-driven SaCas9; U6-driven sgRNA 61592...single cassette expressing human codon-optimized SaCas9; SaCas9 sgRNA can be co-transfected as PCR amplicons...61592 : PX600; CMV-driven SaCas9 60957 : PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression...for sgRNA cloning; has GFP-KASH for FACS sorting SaCas9 + single guide RNA: These plasmids contain two ...two expression cassettes, a human codon-optimized SaCas9 driven by either the ubiquitous cytomegalovirus...inverted terminal repeats (ITR) from AAV serotype 2. SaCas9 only: This plasmid ( PX600, #61592 ) contains a... -
Botman-Teusink Yeast FP Collection
TypeCollection...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast, 21 (8):661-70. https://doi.org...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact, 12 :96. https:... -
Microbiology Resources
TypeCollection...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window... -
CRISPR Guide
TypeCollection...enzyme, additional Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence...meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1... -
Trimmer Lab NeuroMab Collection
TypeCollection...receptor Rat Mouse IgG2a 188151 Anti-Fig4/Sac3 [N202/7R] Fig4/Sac3 Mouse Mouse IgG2a 188153 Anti-Cav1.2 Ca2...Chapsyn-110 Rat Mouse IgG2a 220408 Fig4/Sac3 [N202/38R] Fig4/Sac3 Mouse Mouse IgG2a 220409 TRIP8b (constant... -
Fluorescent Protein Guide: Biosensors
TypeCollection...PLoS One. 2014 Jun 5;9(6):e99095. Joseph Santos-Sacchi Chloride (Cl-) ClopHensorN chloride sensor (cytosolic...sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Biotechnol Biofuels... -
AAV Viral Preps
TypeCollection...Monster, Brainbow Molecular Tools Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR... -
TALEN Guide
TypeCollection...Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently released a TALEN... -
Lentiviral Prep Service
TypeCollection...Catalytically-dead Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID... -
Neurodegeneration Research Collection
TypeCollection...including: Anti-KChIP1 K+ channel [K55/7R] Anti-Fig4/Sac3 [N202/7R] Anti-Ankyrin-G [N106/36] Generate anti-kinesin... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...al., 2011). Follow up work from the Rinehart and Issacs labs at Yale have made improvements to this system... -
CRISPR History and Development for Genome Engineering
TypeCollection...also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged... -
Genetic Code Expansion
TypeCollection...azibutyl-N-carbamoyl-lysine (AbK) Bacterial TAG Andrea Musacchio 127411 pEVOL-pylT-N346A/C348A PylRS M. mazei mono-substituted... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...pyogenes Jaenisch pU6_gRNA_handle_U6t 49016 Mammalian SacI none S. pyogenes EBFP Lu pH1v1 60244 Mammalian Gibson... -
Immunology Research Plasmids and Resources
TypeCollection...class II, major histocompatibility complex, transactivator C2TA, CIITAIV, MHC2TA, NLRA CLEC4M C-type lectin...