Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
Type
Blog Post
...and its 8-bp barcode, respectively.
Once cloned into the storage vector, the pooled, barcoded gRNA library...The CombiGEM platform provides a means to create barcoded gRNA libraries that can be used to combinatorially...restriction digestion and ligation steps to build barcoded lentiviral plasmids containing one or more gRNAs...quantitatively analyzed by sequencing its set of barcodes. CombiGEM is highly flexible and can accommodate...thus be tailored to address the users’ specific research questions. CombiGEM has been successfully applied...Thanks to the tremendous efforts made by various research teams to build and constantly improve gRNA libraries...!
Figure 1: Strategy for assembling the barcoded gRNA library pool. (Adapted from Wong et al., ...