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We narrowed to 653 results for: EPO

Showing: 191 - 200 of 653 results

  1. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing...derived from scientists’ submissions, either their deposited gRNA plasmids or from submission of a spreadsheet...gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently have data...

  2. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ... successful, internationally renowned plasmid repository that it is today. Although I wish I could take... attributed to the entire Addgene family; the depositors and requesting scientists, all of the Addgene... It was great to see the faces of some of our depositing scientists who weren’t able to join us in person...

  3. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...and a domain needed for DNA unwinding. They’ve deposited plasmids for active and inactive CasX for E. coli...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

  4. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...bailii) and B3 (from Zygosaccharomyces bisporus.) Reporter constructs equivalent to site-STOP-site-myrRFP...cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested for excision efficiency...cross-reactivity; however, the other recombinase/reporter pairs were inactive, even at very high recombinase...

  5. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ...Davidson's lab to coordinate the impressively big deposit. “He has every fluorescent protein under the sun...stage IV lung cancer that has now prompted him to deposit the tools he and his team created with the research...team who have made this large, important plasmid deposit possible.   ...

  6. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application.... Transparency window That’s exactly what they reported doing in a paper that appeared in Nature Biotechnology...

  7. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  ...double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage of this...challenging problem than repression, scientists have deposited several plasmid tools that can be used in this...microbiome Beyond the human gut, the Barrick lab has deposited plasmids that can be used to engineer the bee ...

  8. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    ...positive or more negative.  Neuronal stimulation: Depolarization When the inside of the neuron becomes positive...positive over a certain threshold, it becomes depolarized and will fire a signal. The non-specific cation...both cause ion movement that results in cell depolarization. They therefore function as a neuron “on switch...

  9. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...viral infection a) antibody-dependent complement deposition (ADCD) b) antibody-dependent cellular cytotoxicity...., 2019.    Antibody-dependent complement deposition (ADCD) Antibodies can bind to a pathogen and ...opsonization. In antibody-dependent complement deposition (ADCD), opsonization of IgG and IgM antibodies...

  10. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ... the most frequently requested vectors in our repository and will discuss some of the features you may...Some of the more popular studies involve using a reporter vector to tag YGOI with lacZ and/or GFP (e.g. ...animals, gene targeting and genome modifications to reporter vectors (e.g. pBV-Luc) and gene silencing (e.g...
Showing: 191 - 200 of 653 results