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We narrowed to 641 results for: ato

Showing: 191 - 200 of 641 results
  1. The Challenges of Cell Culture

    Type
    Blog Post
    ...simple as a piece of paper on the front of the incubator containing the passage number, cell line, and ...book and on the actual flasks of cells in the incubator). This means any other scientist who wishes to...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...
  2. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    Blog Post
    ...recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (Enabling Discovery ...parenting strategies and make important ecological indicators since species live in water and on land. Current...that tastes yucky (and is poisonous) for bird predators - birds eat a caterpillar once, vomit, and never... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...
  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    ... vector preps expressing the Voltron2 voltage indicator, created by the Schreiter Lab and the GENIE Project... available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they...The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute...
  4. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...platforms to reliably detect nucleic acids at the atomolar level. The Zhang lab has adapted natural RNase...reporter enables SHERLOCK to detect DNA or RNA with atomolar sensitivity and single base pair mismatch specificity... of HPV, HPV16 and HPV18, from human cells, at atomolar levels, within one hour. DETECTR thus has the ...
  5. CRISPR Plasmids - Tagging

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    Collection
    ...Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging Masato Kanemaki's lab has developed a simple and scalable...
  6. Five Popular Model Organisms, Part 2

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    Blog Post
    ...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...characterized genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from.... The Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554/elife.50651...
  7. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...
  8. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...expressing ADAR1-HiBiT were added to LgBiT-expressing hepatocytes or co-cultured across very narrow filters, complementation... of cystic fibrosis transmembrane conductance regulators (CFTR) (Kamada et al., 2023). CFTR is a membrane...cells12232741  Kamio, T., Kono, Y., Hirosuna, K., Ozato, T., Yamamoto, H., Hirasawa, A., Ennishi, D., Tomida...may underlie the heterogeneity and spread of hepatocellular carcinoma in human tissue and in vitro. Oncology...
  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids. Each destination vector ...one. This approach is especially useful for combinatorial analyses. For example, if you were to develop...A versatile modular vector system for rapid combinatorial mammalian genetics.” Journal of Clinical Investigation...Cress, Brady F., et al. “CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9...
Showing: 191 - 200 of 641 results